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A rice glutamine synthase mutant gene for improving plant glufosinate resistance and its preparation method and application

A technique for mutating genes and glutamine, which is applied in the field of rice glutamine synthetase mutant genes and its preparation, can solve problems such as ineffective effects and achieve the effect of enhanced resistance

Active Publication Date: 2015-08-19
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the inhibitory effect of glufosinate on the wild-type glutamine synthetase derived from plants is very obvious, and the effect of transforming this type of gene on improving the resistance of plants to glufosinate-ammonium is not very obvious

Method used

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  • A rice glutamine synthase mutant gene for improving plant glufosinate resistance and its preparation method and application
  • A rice glutamine synthase mutant gene for improving plant glufosinate resistance and its preparation method and application
  • A rice glutamine synthase mutant gene for improving plant glufosinate resistance and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment

[0034] 1. Synthesize a plasmid containing the recombinant rice glutamine synthetase gene

[0035] Using the rice glutamine synthetase gene OsGS sequence (GenBank Accession No. X14245) shown in SEQ ID No1 in the sequence list as a template, 26 primers as shown in SEQ ID No5-SEQ ID No30 in the sequence list were synthesized and named in sequence The primers P1-P26 were amplified by continuous extension PCR method to synthesize a plasmid containing the recombinant rice glutamine synthetase gene.

[0036] The specific synthesis method is as follows:

[0037] Step 1: Use primers P1 and P10 as outer primers, P2-P9 as inner primer synthesis fragment 1; primers P11 and P20 as outer primers, and P12-P19 as inner primer synthesis fragment 2; primers P21 and P26 as outer primers. P22-P25 are the inner primer synthesis fragment 3.

[0038] In the second step, 1 μL fragment 1, fragment 2, and fragment 3 were taken as templates, and amplified with outer primers P1 and P26 to obtain recombinant ric...

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Abstract

The invention discloses a rice glutamine synthetase mutant gene capable of improving resistance of plant glufosinate, the nucleotide sequence of the rice glutamine synthetase mutant gene is shown as SEQ ID No3, and the coded amino acid sequence of the rice glutamine synthetase mutant gene is shown as SEQ ID No4. In addition, the invention also provides the applications of the mutant gene on culturing glufosinate-resistant transgenic crop. The resistance of the gene-coded glutamine synthetase on glufosinate can be obviously enhanced.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a rice glutamine synthetase mutant gene for improving plant glufosinate resistance, and a preparation method and application thereof. Background technique [0002] Glufosinate-ammonium (DL-phosphinotricinm) is the world's second largest genetically modified crop tolerant to herbicides, and glutamine synthetase is the target enzyme of glufosinate-ammonium. [0003] Glufosinate-ammonium has the advantages of low toxicity, easy degradation in the soil, not easy to drift, high activity, low dosage, low environmental pressure and rapid weed killing. However, glufosinate-ammonium is a non-selective herbicide, which will affect the growth of crops while killing weeds, limiting the scope of its use as a herbicide. The bar gene cloned from Streptomyce hygroscopicus and the pat gene cloned from viridochromogenes both encode glufosinate-ammonium acetyltransferase (PAT), which can inactivat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/52C12N9/00C12N15/82C12N15/10A01H5/00
Inventor 赵伟姚泉洪彭日荷薛永朱波许晶高建杰田永生付晓燕金晓芬韩红娟韩静王波王丽娟李振东
Owner SHANGHAI ACAD OF AGRI SCI