Rice glutamine synthetase mutant gene capable of improving resistance of plant glufosinate, and preparation method and applications thereof
A technology of glutamine and mutant gene, which is applied in the field of rice glutamine synthase mutant gene and its preparation, can solve the problem that the effect is not obvious, and achieve the effect of resistance enhancement
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[0034] 1. Synthesis of a plasmid containing the recombinant rice glutamine synthetase gene
[0035] Using the rice glutamine synthetase gene OsGS sequence (GenBank Accession No.X14245) shown in the sequence table SEQ ID No1 as a template, synthesize 26 primers as shown in SEQ ID No5-SEQ ID No30 in the sequence table, named in turn The primers P1-P26 are amplified by the continuous extension PCR method to synthesize a plasmid containing the recombined rice glutamine synthetase gene.
[0036] The specific synthesis method is as follows:
[0037]Step 1: Use primers P1 and P10 as outer primers, P2-P9 as inner primers to synthesize fragment 1; primers P11 and P20 as outer primers, and P12-P19 as inner primers to synthesize fragment 2; primers P21 and P26 as outer primers, P22-P25 is the internal primer synthesis fragment 3.
[0038] In the second step, 1 μL of Fragment 1, Fragment 2, and Fragment 3 were each taken as a template, and the outer primers P1 and P26 were used to ampli...
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