Novel organic microorganism bacterial manure and preparation process thereof
A microbial fertilizer, organic technology, applied in fertilization devices, fertilizer mixtures, applications, etc., can solve problems such as research and application that have not been reported.
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Embodiment 1
[0028] The preparation of embodiment 1 BC39 bacterial powder
[0029] 1 Preparation of strain suspension for inoculation
[0030] Place the BC39 strain stored on a slant at 4°C for activation at 30°C for 1-2 hours, then inoculate it on LB solid plate medium (peptone 10g, yeast extract 5g, sodium chloride 5-10g, agar 20g, pH 7.0, total volume 1000ml), cultured at 30°C for 24h. After the strain grows sufficiently, pick the colony, inoculate it into LB liquid medium, and cultivate it at 30°C and 150rpm for 48h, and set aside
[0031] 2 Bacteria powder preparation
[0032] Fill the liquid fermenter with 1500L / LB liquid culture medium, put it at room temperature for 1-2 days after sterilization, and inoculate 6% of the above-mentioned bacterial suspension respectively after checking for no pollution, and culture at 30°C and 150rpm for 2-3 days, After the fermented liquid is pretreated, the fermented liquid is concentrated by ultrafiltration and spray-dried to obtain bacterial po...
Embodiment 2
[0033] The preparation of other substances in the organic microbial fertilizer of embodiment 2
[0034] Choose peat soil, which is non-toxic to bacterial powder, has good water absorption and water retention performance, pH value between 6.5 and 7.0, small particles, easy to stick to seeds, not easy to agglomerate, and cheap as the matrix carrier. Dry and pulverize the peat soil, sieve through a 100-mesh mesh, and sterilize continuously at 100-121° C. for 30-40 minutes before use.
[0035] Mix peat soil with humic acid and micro-fertilizer, add additives and granulate to form granular fertilizer.
Embodiment 3
[0036] The preparation of embodiment 3 organic microbial fertilizer
[0037] Weigh the BC39 bacterial powder prepared in Example 1, the selected treated peat soil, humic acid, micro-fertilizer, and auxiliary agents according to the following weight ratio.
[0038] The bacterial fertilizer that table 2 organic microbial bacterial fertilizer raw material of the present invention is made in different weight proportions
[0039]
[0040] After uniformly mixing the bacterial powder and the granular fertilizer obtained in step 3, drying and packaging to obtain the product of the present invention.
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