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Immobilized low-temperature nitrobacterium and application thereof in treating low-temperature ammonia-nitrogen wastewater

A technology of ammonia nitrogen wastewater and nitrifying bacteria, applied in the direction of fixing on/in organic carriers, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problem of deteriorating water body ecological environment, water body eutrophication, No problems such as treatment effect, to achieve the effect of avoiding the loss of bacteria, improving microbial activity, and convenient reference

Inactive Publication Date: 2014-05-21
HARBIN GUGUANG ENVIRONMENTAL SCI & TECHCO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After many sewage treatment plants enter winter commissioning and operation, it often takes a long time to adjust to ensure that the ammonia nitrogen reaches the standard. The long-term discharge of ammonia nitrogen that does not meet the standard will cause eutrophication of the water body and deteriorate the ecological environment of the water body.
The study found that when the temperature is low in winter, the sewage treatment efficiency of the sewage treatment station, including aerobic nitrification, drops to about 50%. When the water temperature is lower than 4°C, there is almost no treatment effect; at the same time, low temperature will lead to sludge swelling and other phenomena.

Method used

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  • Immobilized low-temperature nitrobacterium and application thereof in treating low-temperature ammonia-nitrogen wastewater
  • Immobilized low-temperature nitrobacterium and application thereof in treating low-temperature ammonia-nitrogen wastewater
  • Immobilized low-temperature nitrobacterium and application thereof in treating low-temperature ammonia-nitrogen wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 immobilized low-temperature nitrifying bacteria

[0025] (1) Acclimatization of low-temperature nitrifying strains: put the activated sludge from Harbin Taiping Sewage Treatment Plant in a laboratory biochemical incubator and cultivate it at 6~10°C. The main components of the medium are: COD (glucose) 350mg / L, NH 3 + -N(NH 4 CI) is 75mg / L, NaHCO 3 650mg / L, and add a nutrient solution composed of trace elements, so that the final concentration of the added trace elements is CaCO 3 1.0g / L, K 2 HPO 4 0.75g / L, MnSO 4 0.01g / L, MgSO 4 4H 2 00.03g / L; After 3 months of acclimatization and maturity of low-temperature resistant nitrification sludge, the supernatant of domesticated and mature nitrification sludge was separated by a flat-plate streak separation method, and the culture medium used for the isolation of nitrosative bacteria was the improved Stephenson (Stephenson) medium, nitrifying bacteria were isolated using nitrifying bact...

Embodiment 2

[0028] The performance test of embodiment 2 immobilized low-temperature nitrifying bacteria

[0029] 1. Electron microscope observation results

[0030] The surface structure characteristics of the obtained immobilized microbial preparation product (prepared according to the method of Example 1) of the present invention were observed by electron microscope.

[0031] Experiments have proved that the physical and chemical characteristics of the immobilized carrier of the present invention are that the specific surface area increases and can reach 20000m 2 / m3 , Strong water absorption capacity, can absorb 250% of its own weight of water, adjustable density, sedimentation speed, charged load and conductivity. Carrier scanning electron microscope pictures such as figure 2 shown. Electron microscope picture (200×) of the finished product of biosorption mixed immobilized nitrifying bacteria image 3 shown. Electron microscope pictures (6000×) of the finished product of biosorp...

Embodiment 3

[0043] Embodiment 3 Application of the immobilized low-temperature nitrifying bacteria of the present invention in the treatment of low-temperature ammonia-nitrogen wastewater

[0044] The determination of the ammonia nitrogen removal rate of fixed low-temperature nitrifying bacteria is carried out in a small laboratory test to simulate the actual operating conditions of the sewage treatment plant: the cycle operation time is set to 4h, and the time allocation of each stage is generally: water intake + stirring for 20 minutes, water intake + stirring + aeration for 40 minutes, stirring + aeration for 80 minutes, sedimentation for 50 minutes, and drainage for 50 minutes. The test adopts a uniform aeration method, and the aeration volume of each cycle remains unchanged. The end DO of the aeration period is used as the control target. During the test, the end DO is generally controlled to be not less than 4mg / L. The small test device adopts variable volume operation, the MLSS of ...

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Abstract

The invention discloses an immobilized low-temperature nitrobacterium and an application thereof in treating low-temperature ammonia-nitrogen wastewater. The invention provides a method of treating low-temperature ammonia-nitrogen wastewater by using the immobilized low-temperature nitrobacterium. According to the method disclosed by the invention, a mixed low-temperature-resistant nitrobacterium culture can be obtained within a short time, wherein the specific surface area of an immobilized carrier is great (20000<m2> / <m3>), the fixation strength is high, and higher culture activity is retained. The nitrobacterium can be casted at any time into a reactor and is quickly activated. Compared with an existing immobilized low-temperature nitrobacterium technology, the immobilized low-temperature nitrobacterium has the advantages that the sewage treatment temperature is reduced to 5-8 DEG C, so that the nitrobacterium has a huge energy-saving benefit and is suitable for sewage treatment environments of China, and the nitrobacterium has a very high application value.

Description

technical field [0001] The invention relates to biological denitrification wastewater treatment technology, in particular to a method for treating low-temperature ammonia-nitrogen wastewater by using immobilized low-temperature nitrifying bacteria, and belongs to the field of biotechnology. Background technique [0002] When the temperature of sewage in winter is lower than 15°C, the nitrification rate decreases sharply, and when the temperature is around 5°C, the nitrification basically stops, and the nitrification effect becomes poor so that the ammonia nitrogen and total nitrogen cannot meet the discharge standards. With the increasingly stringent discharge standards, this problem is becoming more and more serious. protrude. After many sewage treatment plants enter winter commissioning and operation, it often takes a long time to adjust to ensure that the ammonia nitrogen meets the standard. The long-term discharge of ammonia nitrogen that does not meet the standard will ...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/08C02F3/34
Inventor 朴庸健刘德滨刘敏田辉
Owner HARBIN GUGUANG ENVIRONMENTAL SCI & TECHCO LTD
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