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Breeding method for new line of backcross Crassostrea hongkongensis and hybrid gigas

A breeding method and technology of new strains, applied in the field of cultivation of new strains of fragrant hybrid backcross oysters, can solve the problems of high sterility of hybrid F1, and achieve the effects of easy promotion, strong practicability, and wide temperature range

Active Publication Date: 2014-05-28
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If individuals in fertile F2 hybrids with similar characteristics to F1 hybrids can replace highly sterile F1 hybrids, then the defect of high sterility in F1 hybrids can be solved, and new backcross lines with significant advantages can be produced on a large scale

Method used

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  • Breeding method for new line of backcross Crassostrea hongkongensis and hybrid gigas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] a. Egg preparation: In July 2012, in the Dalian Zhuanghe marine shellfish seedling farm, the Hong Kong oysters that were airlifted from Shenzhen to Dalian in May were used as materials, and placed in the shrimp pond of the farm for ecological ripening. At the beginning of July, the gonads of oysters in Hong Kong were mature, and 15 female individuals were screened out to obtain individual eggs through dissection; each individual egg was filtered through a 200-mesh sieve, and concentrated with a 400-mesh silk screen; then placed in a 2L fresh seawater red bucket for soaking After 30-60 minutes, the germinal vesicles rupture and become mature eggs.

[0021] b. Sperm preparation: Self-propagation using the highly sterile Hong Kong oyster Crassssotrea hongkongensis and long oyster C.gigas hybrid F1 (reference literature: Zhang Yuehuan et al., Hong Kong giant oyster and long oyster interspecific hybridization and early heterosis analysis, Journal of Fisheries, 36(9), 2012, 9...

Embodiment 2

[0025] a. Egg preparation: In June 2012, during the oyster breeding season in Hong Kong, 30 female individuals were screened out in the shellfish seedling field of the Zhanjiang Special Aquatic Animal Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences to obtain individual eggs through dissection; An individual egg is filtered through a 200-mesh sieve, and concentrated by a 400-mesh sieve silk screen; then placed in a 5L fresh seawater red bucket and soaked for 30-60 minutes, the germinal vesicles burst and become mature eggs.

[0026] b. Sperm preparation: using highly sterile Hong Kong oyster and long oyster hybrid F1 for self-propagation, screening out 10 fully fertile male individuals with hybrid F1 genotype in hybrid F2 as materials, and dissected to obtain fresh Single sperm, 100mL semen is obtained by filtering through 500 sieve silk nets, activated by sea water for 5-10min, and actively beating under the microscope is the spe...

Embodiment 3

[0030]a. Egg preparation: In July 2013, during the oyster breeding season in Hong Kong, 36 female individuals were screened out in the shellfish seedling field of the Zhanjiang Special Aquatic Animal Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences to obtain single egg by dissection; An individual egg is filtered through a 200-mesh sieve, and concentrated by a 400-mesh sieve silk screen; then placed in a 5L fresh seawater red bucket and soaked for 30-60 minutes, the germinal vesicles burst and become mature eggs.

[0031] b. Sperm preparation: Using highly sterile Hong Kong oyster and long oyster hybrid F1 for self-propagation, 12 fully fertile male individuals with hybrid F1 genotype in hybrid F2 were selected by molecular markers as materials, and freshly obtained by dissection Single sperm, 100mL semen is obtained by filtering through 500 sieve silk nets, activated by sea water for 5-10min, and actively beating under the micros...

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Abstract

The invention discloses a breeding method for new line of backcross Crassostrea hongkongensis and hybrid gigas. According to the technical links of sperm and ovum preparation, fertilization incubation, filial generation breeding and the like, the characteristic of hybrid F1 male sterile is overcome; a male individual which has the characteristics of a hybrid F1 and is completely fertile in a hybrid F2 is used as a sperm source and is fertilized with a Crassostrea hongkongensis ovum to successfully breed the new line of backcross Crassostrea hongkongensis and hybrid gigas. The breeding method disclosed by the invention is different from the characteristic in the traditional backcrossing that the hybrid F1 is used for backcrossing with a parent, and the hybrid which has the characteristics of a hybrid F1 and is completely fertile in a hybrid F2 is used for backcrossing with the Crassostrea hongkongensis to breed a new line. The new line obtained with the breeding method disclosed by the invention has the advantages of wide temperature applicable range, strong high salinity resistance, obvious growth and survival advantage, simpleness in operation, strong practicality and easiness in popularization, can be bred from north to south, the gigas breeding area is obviously enlarged, and the yield of the new line is improved by 40-150% if being compared with that of the Crassostrea hongkongensis.

Description

Technical field: [0001] The invention belongs to the technical field of shellfish genetics and breeding in marine agriculture, and in particular relates to a method for cultivating a new strain of backcrossed oysters. Background technique: [0002] Interspecific introduction backcrossing, also known as introduction hybridization, improved hybridization, this method is also based on objectively determined breeding objectives such as local natural conditions, economic conditions, production needs and the quality of original local varieties, and introduces a corresponding improved variety that is compatible with the local breed. The improved variety is crossed to obtain the cited F1, and then the cited F1 is used to backcross with the improved variety. This method is just the opposite of the progressive hybridization. In this way, the hybrids of each generation are continuously backcrossed with the improved variety for several generations, and the improved variety still retains...

Claims

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Application Information

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IPC IPC(8): A01K61/00A01K61/54
CPCY02A40/81
Inventor 张跃环喻子牛肖述张扬
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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