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Anti-vibrio alginnolyficus nutrition enhancer for sea cucumbers

A technology of vibrio alginolyticus and enhancer, which is applied in the field of anti-vibrio alginolyticus nutritional enhancer for sea cucumbers and nutritional powder for the prevention and control of pathogenic microorganisms in seafood, achieving the effects of long storage time, less time consumption and low preparation cost

Inactive Publication Date: 2014-05-28
DALIAN DETONG BIO TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Seafood farming is a high-risk farming industry. Traditional small-scale seafood farming has brought huge market and technical risks to farmers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Strain recovery: The colony of the standard strain of Vibrio alginolyticus on the culture medium is smooth, raised, 1-3 mm in diameter, and yellow with neat edges. The standard strain of Vibrio alginolyticus was inoculated in Vibrio alginolyticus culture medium, cultured at 37°C for 18h,

[0028] Wherein, described vibrio alginolyticus culture medium, its preparation method is as follows:

[0029] ① Dissolve 15g of leucine in 100mL of distilled water, filter and sterilize;

[0030] ② Take 3g of yeast extract, 10g of sodium chloride, 2g of sodium cholate, 1g of ferric citrate, 10g of sodium thiosulfate, 1g of calcium chloride, 900mL of distilled water, and sterilize at 121°C for 20min;

[0031] ③ After the medium is gradually cooled, add a sterilized leucine solution;

[0032] ④ Adjust the pH of the solution to 8.0, and cool it down for later use.

[0033] (2) Bacterial enrichment: Pick a loop from the single colony of Vibrio alginolyticus standard strain obtained...

Embodiment 2

[0039] (1) Strain recovery: The colony of the standard strain of Vibrio alginolyticus on the culture medium is smooth, raised, 1-3 mm in diameter, and yellow with neat edges. The standard strain of Vibrio alginolyticus was inoculated in Vibrio alginolyticus culture medium, cultured at 36°C for 18h,

[0040] Wherein, described vibrio alginolyticus culture medium, its preparation method is as follows:

[0041] ① Dissolve 15g of leucine in 100mL of distilled water, filter and sterilize;

[0042] ② Take 3g of yeast extract, 10g of sodium chloride, 2g of sodium cholate, 1g of ferric citrate, 10g of sodium thiosulfate, 1g of calcium chloride, 900mL of distilled water, and sterilize at 121°C for 20min;

[0043] ③ After the medium is gradually cooled, add a sterilized leucine solution;

[0044] ④ Adjust the pH of the solution to 8.0, and cool it down for later use.

[0045] (2) Bacterial enrichment: Pick a loop from the single colony of Vibrio alginolyticus standard strain obtained i...

experiment example 3

[0051] (1) Strain recovery: The colony of the standard strain of Vibrio alginolyticus on the culture medium is smooth, raised, 1-3 mm in diameter, and yellow with neat edges. Inoculate the standard strain of Vibrio alginolyticus in the culture medium of Vibrio alginolyticus, culture at 36±1°C for 16~24h,

[0052] Wherein, described vibrio alginolyticus culture medium, its preparation method is as follows:

[0053] ① Dissolve 15g of leucine in 100mL of distilled water, filter and sterilize;

[0054] ② Take 3g of yeast extract, 10g of sodium chloride, 2g of sodium cholate, 1g of ferric citrate, 10g of sodium thiosulfate, 1g of calcium chloride, 900mL of distilled water, and sterilize at 121°C for 20min;

[0055] ③ After the medium is gradually cooled, add a sterilized leucine solution;

[0056] ④ Adjust the pH of the solution to 8.0, and cool it down for later use.

[0057] (2) Bacteria enrichment: Pick a loop from the single colony of Vibrio alginolyticus standard strain obt...

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Abstract

The invention discloses an anti-vibrio alginnolyficus nutrition enhancer for sea cucumbers. The total weight ratio of an inactivated product to a nutritional feed is 1:(2-10). The preparation process comprises the following steps: bacteria is multiplied and washed to collect bacterial cells; then the bacterial cells are allowed to stand for 2 hours at the temperature of 37 DEG C for hydrolyzing after beta-propiolactone inactivation processing, and is sprayed and dried into the nutritional feed for drying for 24-36 hours; the total weight ratio of the inactivated product to the nutritional feed is 1:(2-10); the product water content is 2-5%; end products are prepared. The anti-vibrio alginnolyficus nutrition enhancer prepared by using the method provided by the embodiment of the invention, is suitable for nursery ponds and culture ponds of the sea cucumbers; the treating process is simple; the time consumption is reduced; the preparation cost is lower than that of the traditional method; the powder has a long preservation time and is free of pollution. The anti-vibrio alginnolyficus nutrition enhancer is of important practical significance for control over vibrio alginnolyficus for large-scale culture of sea cucumbers.

Description

technical field [0001] The invention mainly relates to the technical field of seafood disease detection, in particular to a nutrition powder for prevention and treatment of pathogenic microorganisms in seafood, in particular to an anti-Vibrio alginolyticus nutritional enhancer for sea cucumbers. Background technique [0002] my country has a vast sea area, superior natural conditions, and rich marine resources. But the degree of development and utilization is still very low. This also shows that my country has great potential in the development and utilization of marine resources. Liaoning sea area is an important treasure house of marine biological resources and fishery resources in China. It is rich in sea cucumbers, scallops, mussels, oysters, fish, shrimp and algae, etc., driving the development of marine economy. Strengthen multilateral and bilateral international cooperation in marine development, intensively develop and utilize sea area and island resources, rational...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/00A23K1/10A23K1/14A23K1/16A23K1/175
Inventor 谢克焕
Owner DALIAN DETONG BIO TECH DEV
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