Pueraria lobata extract and application thereof

A technology of kudzu root extract and medicine, which is applied in the field of kudzu root extract, can solve the problem of high price, and achieve the effect of easy industrial production and simple extraction method

Inactive Publication Date: 2014-05-28
邢秋苓
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, a variety of drugs and methods for the prevention and treatment of osteoporosis that inhibit osteoclast formati...

Method used

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  • Pueraria lobata extract and application thereof
  • Pueraria lobata extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] ① Take the dried kudzu root, crush it into small pieces of 1-10mm, and set aside;

[0026] ②The crushed kudzu root was refluxed with 3 times 70% ethanol to extract twice, each time for 1.5 hours, the extracts were combined, refrigerated overnight, filtered, and the filtrate was decompressed to recover ethanol to obtain ethanol extract;

[0027] ③ Add 5 times the amount of distilled water to the ethanol extract to dissolve it, and then extract it with petroleum ether, ethyl acetate, and n-butanol in sequence;

[0028] ④Recover the solvent from the ethyl acetate extract under reduced pressure to obtain extract I;

[0029] ⑤ Dissolve the extract I with 5 times the amount of 90% ethanol, refrigerate overnight, filter, and recover the ethanol from the filtrate under reduced pressure to obtain the kudzu root extract that inhibits osteoclast formation.

[0030] The extract is added to a drug carrier, mixed, granulated and dried to obtain granules. The granule can be used for...

Embodiment 2

[0032] The kudzu root extract obtained in Example 1 was used to determine its effect on osteoclast formation.

[0033] Osteoclast formation system: Multinucleated osteoclasts can be formed by culturing RAW264.7 cells in α-MEM medium for 3-5 days in the presence of RANKL. The formed osteoclasts were observed with tartrate-resistant acid phosphatase (TRAP) staining method to observe TRAP-positive multinucleated cells, and the number of TRAP-positive multinucleated cells with more than 3 nuclei under the microscope was used as an indicator of osteoclast formation.

[0034] The RAW264.7 is a mouse mononuclear / macrophage cell line, which can form into osteoclasts with bone resorption capacity in the presence of osteoclast formation factor (RANKL).

[0035] Pueraria lobata extract was dissolved in α-MEM culture medium, prepared to a certain concentration, and then added to the osteoclast formation system of RAW264.7 cells, and cultured in a cell incubator with 5% carbon dioxide and ...

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Abstract

The invention provides a pueraria lobata extract which is applied to drugs for preventing and treating bone loss diseases. The extract has the function of well inhibiting osteoclast forming and can be used for preparing the drugs for treating bone loss type diseases such as osteoporosis. The extract is obtained through the following steps: performing reflux extraction on dried pueraria lobata through ethanol, recovering ethanol to obtain extractum, dissolving the extractum by using distilled water and then leaching by using an organic solvent, and recovering the solvent after the extract is dissolved by ethanol. The pueraria lobata extract provided by the invention has the function of well inhibiting osteoclast forming, is from natural medicines, has few side effects and is safe in use.

Description

technical field [0001] The present invention relates to the extract of Pueraria root, in particular to the application of the extract of Pueraria root in the preparation of medicines for preventing and treating bone loss diseases. Background technique [0002] With the aging of the world's population, the incidence of osteoporosis is increasing year by year. The bone pain and fractures caused by osteoporosis directly affect the quality of life of middle-aged and elderly people, and have become serious social problems. Human bones are constantly undergoing remodeling through continuous bone formation and bone resorption, which is called bone remodeling. Osteogenesis and bone resorption maintain a good balance when young, but as we age, the balance tends to bone resorption due to various reasons, and if this state continues for a long time, the bone tissue becomes brittle, leading to osteoporosis Diseases, fractures and other bone diseases. In the human body, osteoclasts are...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P19/02A61P19/08A61P19/10A61P29/00
Inventor 邢秋苓
Owner 邢秋苓
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