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A kind of silk fibroin-based double-layer antibacterial film and preparation method thereof

A technology of antibacterial film and silk fibroin, which is applied in the field of medical biomaterials and its preparation, can solve the problems of uncontrollable usage, difficult uniformity of pearl powder, hidden safety hazards, etc., and achieves benefits for wound healing, inhibition of bacterial growth, and convenient use Effect

Inactive Publication Date: 2015-09-23
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since this nano-wound patch is a water-soluble film, if there is fluid in the wound surface, it will dissolve, and the nano-silver may enter the body through the wound surface and cause harm to the body. Therefore, this nano-wound patch has potential safety hazards. In addition, Direct external application of pearl powder is difficult to apply evenly on the wound surface, the amount of use is difficult to control, and it is easy to scatter

Method used

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  • A kind of silk fibroin-based double-layer antibacterial film and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Preparation of silk fibroin solution

[0019] After removing the sericin from the waste silk, dissolve it in the ternary solution of calcium chloride, ethanol and deionized water, and obtain a silk fibroin solution with a mass concentration of 2.5% to 6% through dialysis and filtration.

[0020] 2. Preparation of nano-silver film: Add 0.9096 mg of nano-silver to a beaker containing 5 mL (0.1516 g) silk fibroin solution, disperse with ultrasonic waves for 15 min, then add 0.0303 g of glycerin, and stir for 5 min while ultrasonically treating, Pour it into a mold, and dry it to form a film in an environment with a temperature of 20°C and a relative humidity of 45%.

[0021] 3. Preparation of pearl powder-containing film: Add 0.0152g pearl powder to a beaker containing 5mL (0.1516g) silk fibroin solution, disperse it with ultrasonic wave for 20min, then add 0.0303g glycerin, and stir for 6min while ultrasonic treatment, Pour it into a mold with a dried nano-silver film, ...

Embodiment 2

[0024] Silk fibroin solution was prepared according to Example 1.

[0025] For the preparation of nano-silver film, 0.932 mg nano-silver was added to a beaker containing 5 mL (0.1553 g) silk fibroin solution, dispersed by ultrasonic wave for 20 min, then 0.0311 g glycerin was added, and stirred for 5 min while ultrasonic treatment , poured into a mold, and dried to form a film in an environment with a temperature of 22°C and a relative humidity of 50%.

[0026] The preparation of film containing pearl powder, the pearl powder of 0.0233g is added in the beaker that 5mL (0.1553g) silk fibroin solution is housed, disperses with ultrasonic wave 25min, then adds 0.0311g glycerin, after stirring 8min while ultrasonic treatment, Pour it into a mold with a dried nano-silver film, use the self-assembly principle of silk fibroin to dry the film in an environment with a temperature of 22 ° C and a relative humidity of 50%, and connect the upper and lower layers of the film.

[0027] The...

Embodiment 3

[0029] Silk fibroin solution was prepared according to Example 1.

[0030] The preparation of the film containing nano-silver layer, the nano-silver of 1.2mg is added in the beaker that is equipped with 5mL (0.150g) silk fibroin solution, disperses with ultrasonic wave 18min, then adds 0.0225g glycerol, after ultrasonic treatment while stirring 10min , poured into the mold containing the dried pearl powder film, and dried to form a film in an environment with a temperature of 18°C ​​and a relative humidity of 35%.

[0031] For the preparation of the film containing pearl powder, add 0.03g pearl powder into a beaker containing 5mL (0.150g) silk fibroin solution, disperse with ultrasonic waves for 30 min, then add 0.03g glycerin, and stir for 5 min while ultrasonically treating, Pour it into a mold, and use the self-assembly principle of silk fibroin to dry and form a film in an environment with a temperature of 18°C ​​and a relative humidity of 35%, and at the same time connect...

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Abstract

The invention relates to a fibroin based double-layer antibiotic film and a making method thereof. The making method of the film comprises the following steps: respectively adding nano-Ag, pearl powder and glycerin into a fibroin solution, and carrying out drying film formation at a certain temperature under a certain humidity; and the conversion of silk fibroin from aperiodical coil to a silk I structure is induced by glycerin in the drying film formation process, and the self-assmbling of the silk fibroin enables two films to be connected. When the antibiotic film is used, a film containing nano-Ag is used as an external layer film, so the invasion of external bacteria to wounds can be blocked, and the latent body harms caused by the direct contact with skins can also be avoided; and a film containing the pearl powder is used as an internal layer, so the non-uniform scattered laying of the pearl powder can be avoided, and the inhibition of the growth of bacteria on the whole wounds is realized. The fibroin based double-layer antibiotic film is insoluble in water, and overcomes the latent biological safety of nano-Ag and the limit bacteriostasis of the pearl powder, and the silk fibroin in the film, and pearl proteins and trace elements comprising strontium, manganese and zinc in the pearl powder are in favor of healing the wounds.

Description

technical field [0001] The invention relates to a medical biological material and a preparation method thereof, in particular to a double-layer antibacterial film with silk fibroin as a matrix, pearl powder and nano-silver as an antibacterial agent, and glycerin as a plasticizer and a preparation method thereof. Background technique [0002] Silk fibroin is an excellent natural high-molecular fiber protein extracted from silk, which is non-toxic, non-sensitizing and non-irritating, and has excellent biocompatibility and degradability. Silk fibroin has properties similar to collagen and can promote cell growth. Therefore, silk fibroin can be used as a cell culture substrate, wound covering material and artificial skin, etc. (see literature: 1, B. B. Mandal, S. C. Kundu. Biospinning by silkworms: Silk fiber matrices for tissue engineering applications. Acta Biomaterialia 6 (2010) 360–371; 2. S. Kanokpanon, S.Damrongsakkul, J. Ratanavaraporn, P. Aramwit. An innovative bi-layere...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/32A61L15/44B32B33/00
Inventor 谢瑞娟张萌
Owner SUZHOU UNIV