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Achromobacter xylosoxidans and application thereof

A colorless bacillus and xylose technology, applied in the field of environmental microorganisms, can solve the problems of secondary pollution, high cost of chemical oxidation/reduction, and insufficient efficiency, and achieve strong resistance and adaptability, easy to be removed by adsorption, and strong mobility sexual effect

Inactive Publication Date: 2014-06-04
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Physical methods such as adsorption, filtration, and guest-soil methods are time-consuming and costly, and are not conducive to large-scale purification; chemical precipitation and chemical oxidation / reduction are costly and easy to cause secondary pollution; phytoremediation methods (such as centipede grass) can remove soil Medium and high concentrations of arsenic pollutants are enriched in the body, but it is not suitable for the treatment of arsenic-contaminated groundwater, and the workload of planting and subsequent treatment of arsenic hyper-accumulated plants is relatively large; the microbial remediation method mainly uses microorganisms to oxidize heavy metal ions Metabolism such as / reduction, methylation / demethylation can achieve the purpose of arsenic pollution control. It is a cheap, safe and most effective repair method without causing damage to the environmental structure, which meets the needs of sustainable development. Currently the most promising arsenic pollution control method (Cai Lin, Wang Gejiao. Purification of arsenic-polluted Achromobacter xylosoxidans SY8 and its application. 2007, patent number: ZL200710052064.6)
However, this method often needs to combine the strong adsorption of pentavalent inorganic arsenic by soil or activated sludge to achieve a high removal rate, and the highly toxic trivalent arsenic in the environment is not easy to remove from the environment because it has no charge. It is urgent to efficiently oxidize trivalent arsenic to pentavalent arsenic in waste water, sewage irrigation water, and soil, but the chemical oxidation method is often not efficient enough. In practice, it is urgent to solve this technical bottleneck. High-efficiency arsenic-oxidizing bacteria are an excellent s Choice

Method used

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  • Achromobacter xylosoxidans and application thereof
  • Achromobacter xylosoxidans and application thereof
  • Achromobacter xylosoxidans and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Example 1: Isolation of Achromobacter xylosoxidans GD03 from arsenic-contaminated simulated soil

[0017] The experimental soil was taken from the surface soil of a rice field soil in Fuzhou City, Fujian Province, and NaAsO was added at the ratio of 1 g As(III) / kg soil 2 , After mixing well, place it in the dark for 3 months to obtain a simulated soil sample of arsenic pollution. The specific operation steps are as follows:

[0018] (1) Enrichment of arsenic-resistant bacteria: accurately weigh 1 g of arsenic-contaminated simulated soil sample into a 150 ml Erlenmeyer flask containing 25 mg As(III) / L sterile enrichment medium, and culture at 30°C on a shaker (128 rpm) for one week, observe the growth of the strain. When the medium appears turbid, it is sequentially inoculated into the next enriched medium with a higher As(III) concentration at a ratio of 10% (volume ratio). The As(III) concentration is 50 mg / L and 100 mg / L, respectively. L, 150 mg / L, 200 mg / L, 400 mg / L to ...

Embodiment 2

[0025] Example 2: Arsenic oxidation curve of Achromobacter xylosoxidans GD03

[0026] Pick a single clone of Achromobacter xylosoxidans GD03, inoculate it into 100ml of the optimal liquid medium E containing 100mgAs(III) / L, place it in a shaker at 28°C for 48 hours with shaking, and draw from it at 5% of the inoculum 5ml to 100ml of fresh optimal liquid medium E containing 150mgAs(III) / L, culture in a shaker at 28℃ for 24 hours, and then draw 5ml to 100ml of fresh optimal liquid culture containing 200mgAs(III) / L In base E, place it in a shaker at 28°C and shake for 24 hours. At this time, the OD of GD03 600 It is about 0.8, stored in a refrigerator at 4°C and used as seed bacterial solution for inoculation. Draw 5ml to 100ml of fresh optimal liquid medium E containing 1000mgAs(III) / L with a 5% inoculum amount, and place it in a shaker at 28°C for 9 hours and start sampling, every 1 hour Take one sample until As(III) is completely oxidized, take 1.2ml and 0.5ml each time, and me...

Embodiment 3

[0027] Example 3: Oxidation effect of Achromobacter xylosoxidans GD03 on trivalent arsenic in simulated arsenic-contaminated rice hydroponics

[0028] Using conventional rice N, P, K nutrient hydroponics (containing N 23mg / L, P 7mg / L, K 21mg / L) as a control, referring to my country’s maximum arsenic-contaminated groundwater level is about 2-3 mg / L, Add NaAsO to rice hydroponic nutrient solution 2 To make the trivalent arsenic concentration in the hydroponic solution reach 3mg / L, design different inoculation ratios of Achromobacter xylosoxidans GD03 in the rice hydroponic nutrient solution, and investigate the oxidation effect of GD03 on trivalent arsenic. The specific method is as follows: prepare 8 250ml Erlenmeyer flasks, 4 for 100ml conventional rice N, P, K nutrient hydroponics (sterilized at 121℃ for 20 minutes), and the other 4 for 100ml non-sterilized rice conventional N, P , K nutrient hydroponics, according to the inoculation ratio (volume ratio) of 0%, 0.25%, 0.50% and ...

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Abstract

The invention relates to a strain of achromobacter xylosoxidans GD03 with oxidation effect and methylation effect on inorganic trivalent arsenic, and a preliminary application in repair of seedling-stage paddy rice arsenic poisoning. The strain was preserved in China General Microbiological Culture Collection Center (CGMCC) at Jan 26th, 2013, and has a preservation number of CGMCC No. 8512. The achromobacter xylosoxidans of the invention is characterized in that the achromobacter xylosoxidans GD03 with oxidation effect and methylation effect on inorganic trivalent arsenic is obtained by enrichment and separation from arsenic-polluted simulated rice field soil. The strain can oxidize inorganic trivalent arsenic in arsenic-polluted environment into pentavalent arsenic, has methylation effect, greatly reduces the toxicity of trivalent arsenic with high toxicity in the environment, and enhances the adsorbable removing property of arsenic pollutants.

Description

technical field [0001] The invention belongs to the technical field of environmental microbes, and in particular relates to the screening of an Achromobacter xylosoxidans GD03 strain capable of oxidizing inorganic trivalent arsenic and its use in alleviating arsenic poisoning in rice seedling growth. Background technique [0002] Arsenic pollution has become a serious environmental problem facing the world. According to reports, arsenic pollution is the most serious in Asian countries in the world, especially Bangladesh, India, China, etc. (Y. G. Zhu, et al. Exposure to inorganic arsenic from rice: A global health issue Environmental Pollution, 2008, 154(2) : 169-171). In my country, due to factors such as mine emissions and various arsenic-containing chemicals used in industrial and agricultural production, the arsenic content in soil, groundwater, and drinking water continues to increase, and finally passes through "soil / water → plant body / animal body → human body". The f...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/025A62D101/43
Inventor 杨桂娣邱宗清黄怡杨孝军林文雄
Owner FUJIAN AGRI & FORESTRY UNIV
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