Silk-based bioactive oligopeptide compositions and manufacturing process therefor

A technology for biological activity and synthesis, which can be used in drug combinations, peptide preparation methods, peptide/protein components, etc., and can solve problems such as unfavorable bitterness, expensiveness, complexity, etc.

Inactive Publication Date: 2014-06-04
AGRI RES DEV AGENCY PUBLIC ORG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Examples of these sub-processes include desalination or nanofiltration, which are complex, time-consuming, and/or expensive
Moreover, i

Method used

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  • Silk-based bioactive oligopeptide compositions and manufacturing process therefor
  • Silk-based bioactive oligopeptide compositions and manufacturing process therefor
  • Silk-based bioactive oligopeptide compositions and manufacturing process therefor

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0038] The experiment evaluated the effect of the special sericin-derived oligopeptide provided by the present invention on the blood sugar level of diabetic rats, which were 8-week-old Wistar male rats weighing 180-200 g. Diabetes was induced using a single intravenous (i.v.) injection of streptozotocin at a concentration of 55 mg / kg body weight (BM). On day 7, rat blood glucose levels were determined by blood sampling and a glucose testing kit (ACCUCHEK, Roche Laboratory Pharma). Rats with blood glucose levels above 300 mg / dl were selected for further experimental steps in the diabetic group.

[0039] In Example One, rats were sorted into groups 1-6, with 6 rats in each group, as follows:

[0040] (i) Group 1: Normal control rat group, which was administered distilled water.

[0041] (ii) Group 2: Diabetic control rat group, which was administered distilled water.

[0042] (iii) Group 3: Diabetic rats were given a predetermined volume of Glibenclamide alone (the standard ...

example 2

[0053] The test evaluated the effect of the specific sericin-derived oligopeptides provided by the present invention on blood pressure reduction in diabetic rats, which were 8-week-old Wistar male rats weighing 180-200 grams.

[0054] Rats were sorted into groups 1-3, with 6 rats per group, as follows:

[0055] (i) Group 1: Control group, rats were administered distilled water.

[0056] (ii) Group 2: administered via femoral vein injection at concentrations ranging from 10 -7 -10 -1 mg / ml (ie, 10 -7 、10 -6 、10 -5 、10 -4 、10 -3 、10 -2 , and 10 -1 mg / ml), the predetermined volume is the oligopeptide provided by the present invention. According to the oligopeptide of the present invention, a certain dose of the minimum oligopeptide concentration (ie, 10 -7 mg / ml), and then monitor the blood pressure of the rats. After the blood pressure value returned to the initial value, a second higher dose (i.e., 10 -6 mg / ml) oligopeptide concentration. This process was repeated ...

example 3

[0066] The tests evaluated the antioxidant properties or effects of specific sericin-derived oligopeptides according to the invention. It is well known in the art that the oxidative degradation of lipids involves lipid peroxidation. The antioxidant properties of the oligopeptides provided by the present invention were determined by measuring the degree of oxidation of low density lipoprotein (LDL) isolated from plasma.

[0067] A predetermined volume of human LDL with a density of 1.019-1.063 g / ml was separated from the plasma of healthy volunteers by density continuous ultracentrifugation. Once dialyzed in phosphate-buffered saline containing 10 μM ethylenediaminetetraacetic acid (EDTA), the LDL protein concentration was determined by BCA ( )to make sure. LDL was stored at 4°C under nitrogen until used in the lipid peroxidation step.

[0068] Sericin-derived oligopeptides of varying concentrations provided by the present invention were dissolved in water and incubated wit...

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PUM

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Abstract

A process for preparing or manufacturing target molecular weight silk-derived oligopeptides with bioactive properties includes providing silk cocoon, extracting proteins or peptides from the silk cocoon, introducing an enzyme solution to obtain oligopeptides, and separating target oligopeptides from other peptides. Silk-derived oligopeptides in accordance with the present disclosure possess one or more of antioxidant properties, cholesterol lowering activity, lipid oxidation prevention properties, blood sugar lowering properties, blood pressure lowering properties, natural killer cell enhancement activity, insulin production enhancement activity, body weight regulatory activity, central nervous system stimulant properties, and neuroprotective, cognitive function protective, memory protective, and/or neural function improvement properties (e.g., memory deficit alleviation, spatial memory improvement, recognition memory improvement, learning improvement, synaptic transmission facilitation, and anti -neurodegenerative properties).

Description

technical field [0001] The present invention relates to an enhanced bioactive oligopeptide, and in particular to a sericin-based oligopeptide, and a simplified, short-term and safe process for producing the sericin-based oligopeptide, thereby satisfying A target oligopeptide molecular size or molecular weight profile that enhances or maximizes biological activity or a property of biological activity. Background technique [0002] Various protein-rich products exist in the form of protein hydrolysates. Protein hydrolysates are peptide mixtures prepared by acid, alkali, or enzymatic protein cleavage that provide nutritional equivalents of the original materials in relation to the constituent amino acids, and are used in (for example, for those who do not have access to common food protein, or who experience fatigue conditions patients) as a nutrient and fluid supplement in specific diets. [0003] Peptides with molecules of 2-50 amino acids and molecular weights below 5,500 ...

Claims

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Application Information

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IPC IPC(8): C07K1/12C07K7/06C07K5/00A61K38/08A23L2/66
CPCC07K14/43563C12P21/06C07K5/00C07K14/43586A23L2/66A23L1/3053C07K1/12A61K35/642C07K7/06A23L33/18A61K35/63A61P3/00A61P3/04A61P3/10A61P9/12A61P25/00A61P25/28A61P37/00A61P43/00A61K38/08C07K1/145
Inventor 曼努特·苏斯拉瓦塔纳农达南丁提·里姆宾兆查纳容·托查鲁斯瓦里·提亚博查度昂卡莫·坎萨勒博纳林·泰帕瓦拉普鲁克基拉博·托查鲁斯罗苏坤·厚查拉特苏帕斯特·帕纳鲁诺泰
Owner AGRI RES DEV AGENCY PUBLIC ORG
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