Hydrophobic solid microspheres containing epidermal growth factors and preparation method and application of microspheres

A technology of epidermal growth factor and solid, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of maintaining EGF activity and not solving for a long time, and achieves simple preparation process, no three wastes, and easy use Effect

Active Publication Date: 2014-06-18
GUANGDONG VOCATIONAL & TECHNICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of these patents or patent applications address the problem of maintaining EGF activity for long periods of time

Method used

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  • Hydrophobic solid microspheres containing epidermal growth factors and preparation method and application of microspheres
  • Hydrophobic solid microspheres containing epidermal growth factors and preparation method and application of microspheres
  • Hydrophobic solid microspheres containing epidermal growth factors and preparation method and application of microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Add 10 g of polyethylene glycol (PEG-2000, white solid, melting point 51±2°C) into 100 mL of deionized water, stir at 400 rpm at 0°C until completely dissolved, freeze at -5°C and then lyophilize. After ball milling at -10°C, pass through a 115-mesh sieve (125 μm) to obtain polyethylene glycol porous microspheres.

[0034] At 0°C, mix 1 g of polyethylene glycol porous microspheres and 0.1 g of epidermal growth factor powder (purchased from Sigma; name: EGF Epidermal cell growth factor; property: lyophilized powder, BioReagent, suitable for cell culture; CAS number: 62229-50-9) into a three-dimensional mixer, first stirred at a low speed of 600rpm for 1min, and then stirred at a high speed of 1200rpm for 1min to obtain polyethylene glycol porous microspheres containing epidermal growth factor.

[0035] At 0°C, spread 1g of polyethylene glycol porous microspheres containing epidermal growth factor on the vibrating conveyor belt, and melt paraffin (section paraffin, pathol...

Embodiment 2

[0038] Add 10g of polyethylene glycol (PEG-4000, white solid, melting point 55±2°C) into 200mL of deionized water, stir at 300rpm at 5°C until completely dissolved, freeze at -80°C and then lyophilize. After ball milling at -20°C, pass through a 115-mesh sieve (125 μm) to obtain polyethylene glycol porous microspheres.

[0039] At 5°C, mix 1 g of polyethylene glycol porous microspheres and 0.2 g of epidermal growth factor powder (purchased from Sigma; name: EGF Epidermal cell growth factor; property: lyophilized powder, BioReagent, suitable for cell culture; CAS number: 62229-50-9) into a three-dimensional mixer, first stirred at a low speed of 500rpm for 2 minutes, and then stirred at a high speed of 2000rpm for 3 minutes to obtain polyethylene glycol porous microspheres containing epidermal growth factor.

[0040] At 5°C, spread 1g of polyethylene glycol porous microspheres containing epidermal growth factor on a vibrating conveyor belt, and melt paraffin (section paraffin, ...

Embodiment 3

[0042] Add 10g of polyethylene glycol (PEG-6000, white solid, melting point 57±2°C) into 400mL of deionized water, stir at 200rpm at 15°C until completely dissolved, freeze at -65°C and then lyophilize. After ball milling at -15°C, pass through a 115-mesh sieve (125 μm) to obtain polyethylene glycol porous microspheres.

[0043] At 15°C, 1 g of polyethylene glycol porous microspheres and 0.1 g of epidermal growth factor powder (purchased from Sigma; name: EGF Epidermal cell growth factor; properties: lyophilized powder, BioReagent, suitable for cell culture; CAS number: 62229-50-9) into a three-dimensional mixer, first stirred at a low speed of 300rpm for 4 minutes, and then stirred at a high speed of 2500rpm for 4 minutes to obtain polyethylene glycol porous microspheres containing epidermal growth factor.

[0044] Spread 1g of polyethylene glycol porous microspheres containing epidermal growth factor on a vibrating conveyor belt at 15°C, and melt paraffin (section paraffin, ...

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Abstract

The invention discloses hydrophobic solid microspheres containing epidermal growth factors and a preparation method and application of the microspheres. The preparation method comprises the following steps: with polyethylene glycol porous microspheres as a protective agent, loading the epidermal growth factors; and protecting the polyethylene glycol porous microspheres loading the epidermal growth factors by paraffin through fusing and spraying. The preparation method provided by the invention has the advantages and effects that (1) polyethylene glycol with the molecular weight from 2000 to 20000 is selected as the protective agent for the epidermal growth factors; (2) the epidermal growth factors are protected by solid paraffin, and polyethylene glycol freezed powder isolates water; (3) the preparation process is simple and does not refer to chemical reactions, and is free of the generation of the three wastes and green and environment-friendly; (4) the contained epidermal growth factors are acid-resistant, alkali-resistant, light-resistant, waterproof and high-temperature-resistant; and (5) the hydrophobic solid microspheres are simple and convenient to use, and can serve as a functional additive which is added into various emulsions and cream or frost-like cosmetics by being slightly squeezed.

Description

technical field [0001] The invention belongs to the field of functional materials, in particular to a hydrophobic solid microsphere containing epidermal growth factor, its preparation method and its application in cosmetics. Background technique [0002] Epidermal Growth Factor (EGF for short, CAS: 62229-50-9) is a class of polypeptide substances widely present in mammals, secreted by normal cells, and has neither drug toxicity nor immune response, so it is It is widely used in human burns, wounds, diabetic skin ulcers, bedsores, varicose skin ulcers and corneal injuries to promote wound healing. Its main functions in the field of beauty are: ①Skin rejuvenation. EGF can stimulate various cells derived from ectoderm and endoderm, such as corneal epithelial and endothelial cells, epidermis, dermis cells (such as fibroblasts), mammary acini and interstitial cells, etc., to make them proliferate and migrate, and accelerate metabolism. Achieve skin rejuvenation effect. ② Moist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61K8/84A61K8/02A61K8/06A61Q19/00
Inventor 吴婷蔡祥马栋刘宏喜谭绍早薛巍吴志敏
Owner GUANGDONG VOCATIONAL & TECHNICAL COLLEGE
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