A kind of sea cucumber peptide stabilized preparation and preparation method and application thereof
A technology for stabilizing preparations and sea cucumber peptides, which is applied in the field of sea cucumber peptide stabilizing preparations and its preparation, to achieve the effects of enhancing skin elasticity, stable state, and rich nutritional value
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Embodiment 1
[0014] Embodiment 1 prepares sea cucumber peptide stable preparation
[0015] Take 1 kg of fresh sea cucumber, wash, crush, hydrolyze with protease, ethanol precipitation, and concentrate and dry the supernatant to obtain sea cucumber peptide (51 g) (the sea cucumber peptide is prepared according to the method of Example 1 in Patent Application No. 200910014298.0, which is used in the present invention sea cucumber peptide), sea cucumber peptide was dissolved in distilled water to obtain 9.5kg of sea cucumber peptide solution, sterilized by high pressure steam, and filtered to obtain the supernatant, which was divided into two parts on average, one of which was added with ultra-low molecular weight hyaluronic acid ( 8000 Daltons) 5g and trehalose 150g, after stirring evenly, add distilled water to make up to 5kg, and fill it to obtain a sea cucumber peptide stable preparation (sea cucumber peptide mass concentration 0.51%, hyaluronic acid concentration 0.1%, trehalose concent...
Embodiment 2
[0022] Example 2 Sea Cucumber Peptide Stabilized Preparation Used as Evaluation of Feeling in Use of Skin Care Water
[0023] Test method: 90 female subjects were divided into six groups: purified water control group, sea cucumber peptide liquid group (prepared in Example 1), trehalose group (3% trehalose aqueous solution by mass percentage), ultra-low molecular hyaluronic acid group ( 0.1% 8000 Dalton hyaluronic acid aqueous solution by mass), trehalose + ultra-low molecular hyaluronic acid group (3% trehalose + 0.1% 8000 Dalton hyaluronic acid aqueous solution by mass), sea cucumber peptide stabilized preparation Group (prepared in Example 1), each group has 15 people. The solutions of each test group were evenly applied to the faces of the subjects in the test group (1ml per person), and used continuously for 7 days. The control group was coated with the same amount of pure water and used continuously for 7 days. After 7 days, the feeling of use, skin elasticity, and mois...
Embodiment 3
[0028] The evaluation of embodiment 3 sea cucumber peptide stable preparations to skin elasticity effect
[0029] Test method: Divide 120 female subjects aged 30 to 50 into 8 groups: purified water control group, sea cucumber peptide solution group (prepared in Example 1), trehalose group (3% trehalose aqueous solution by mass percentage), super Low molecular hyaluronic acid group (0.1% by mass 8000 Dalton hyaluronic acid aqueous solution), trehalose + ultra-low molecular hyaluronic acid group (3% by mass trehalose + 0.1% by mass 8000 Dalton hyaluronic acid aqueous solution), sea cucumber peptide stable preparation group (prepared in Example 1), 15 people in each group. The solutions of each group were applied to the faces of the group members, twice a day, 1ml each time. After 6 weeks, Dermascan C. was used to measure dermal firmness for comparative analysis of skin condition. The results are shown in Table 4 (the numbers in the table represent the increase rate of skin ela...
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