Microbial oil and preparation method thereof
A technology for microbial oil and oil-producing microorganisms, applied in food preparation, fat oil/fat refining, food science, etc., can solve the problems of high surface oil content, poor emulsification performance of microbial oil, unfavorable production and application, etc. content, improving antioxidant capacity, and prolonging shelf life
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Embodiment 1
[0033] Taking Mortierella alpina as the starting strain, the production and application of the microbial oil containing arachidonic acid of the present invention are described in detail.
[0034] 1. Fermentation
[0035] Prepare a medium solution with a glucose content of 0.03g / mL and a yeast powder content of 0.02g / mL in a 500ml shaker flask. Multiple bottles can be prepared. After sterilization, insert appropriate amount of Mortierella alpina mycelia and spores, and place at 28°C Constant temperature shaker, 150rpm, after 2 days, merge the shaker flasks, and transfer them into a 1m medium solution containing 0.03g / mL glucose content and 0.02g / mL yeast powder content that has been sterilized. 3 In the fermenter (first-class seed tank), sterile air is continuously introduced to keep the culture temperature at 28±2°C. After 2 days of cultivation in the first-level seed tank, transfer all the culture solution into a 10mL medium solution with a glucose content of 0.03g / mL and a ...
Embodiment 2
[0095]Taking Schizochytrium as the starting strain, the production and application of the microbial oil containing docosahexaenoic acid of the present invention are described in detail.
[0096] 1. Fermentation
[0097] Prepare a culture medium solution with a glucose content of 0.04g / mL and a yeast extract content of 0.02g / mL in a 1000ml shaker flask. Multiple bottles can be prepared. After sterilization, add an appropriate amount of refrigerated Schizochytrium bacteria solution and place it at a constant temperature of 28°C for shaking Bed, 180rpm for activation. After 2 days, they were inserted into the secondary expansion shaker flasks for cultivation. After 2 days, the shaker flasks were merged and transferred into a sterilized 1m medium containing 5wt% glucose and 2wt% yeast extract. 3 In the fermenter (first-level seed tank), sterile air was continuously introduced to keep the culture temperature at 29±1°C. After 2 days of cultivation in the primary seed tank, transfe...
Embodiment 3
[0126] Taking Pythium ultima as the starting strain, the production and application of microbial oil containing eicosapentaenoic acid are described in detail.
[0127] 1. Fermentation
[0128] Prepare a medium solution with a sucrose content of 0.05g / mL and a yeast powder content of 0.005g / mL in a 1000ml shaker flask. Multiple bottles can be prepared. After sterilization, add an appropriate amount of Pythium ultima and place it on a constant temperature shaker at 28°C. Activation was performed at 180 rpm. After 2 days, insert the secondary expansion flask for culture, combine the shake flasks after 2 days, and transfer into the sterilized 1m 3 In the fermenter (first-class seed tank), sterile air was continuously introduced to keep the culture temperature at 28±1°C. After 2 days of cultivation in the primary seed tank, transfer all the culture solution into a sterilized 10mL container with 0.05g / mL sucrose and 0.005g / mL yeast powder. 3 In the fermenter (secondary seed tank)...
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