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Protein meal with function of promoting bone growth and preparation method of protein meal

A technology of bone growth and protein powder, applied in the direction of protein composition of milk, food preparation, and function of food ingredients

Active Publication Date: 2014-07-02
王猛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the promotion of bone growth and development is a complex process of dynamic balance between osteoblasts and osteoclasts. The product preparation technology developed for this complex biological process is blank at home and abroad.

Method used

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  • Protein meal with function of promoting bone growth and preparation method of protein meal
  • Protein meal with function of promoting bone growth and preparation method of protein meal
  • Protein meal with function of promoting bone growth and preparation method of protein meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Such as figure 1 As shown, the present embodiment prepares protein powder with the activity of promoting osteoblast proliferation according to the following steps:

[0040] (1) Milk sample processing: Centrifuge fresh milk at 5000-10000rpm for 15-30min to skim, adjust the pH value of the skim milk to 4.3-4.8 with acid, let it stand for 10-30min, centrifuge at 4000-8000r / min for 1-30min, collect The supernatant after centrifugation was filtered.

[0041] In this step, the acid is hydrochloric acid, sulfuric acid or acetic acid.

[0042] In this step, the concentration of the acid is 0.1-3mol / L.

[0043] In this step, the filtration method is as follows: the supernatant is filtered through filter membranes with a pore size of 0.45 μm and 0.22 μm in sequence, and all operations are carried out at 2-6° C.

[0044] (2) Purification by cation exchange chromatography: the sample obtained in step (1) was first purified by strong cation exchange chromatography SP Sepharose Bi...

Embodiment 2

[0062] In this embodiment, the protein powder having the activity of promoting osteoblast proliferation is prepared according to the following steps:

[0063] (1) Milk sample processing: Centrifuge fresh milk at 5000rpm for 15min to defat. Use 0.5mol / L hydrochloric acid to adjust the pH value of the skim milk to 4.5, let it stand for 15 minutes, and then centrifuge at 6000r / min for 10 minutes. Carried out at 2-6°C.

[0064] (2) Purification by cation exchange chromatography: the sample was first purified by strong cation exchange chromatography SP Sepharose Big Bead (1.6×50 cm). Equilibrate with 200mL, 50mmol / L phosphate solution (buffer solution A) with a pH value of 7.1 in advance. Buffer solution B is buffer solution A containing 0.2mol / L NaCl. Two-stage step-by-step elution was adopted, using 300 mL of a mixed solution containing 20 vol.% buffer solution B for elution, and then 150 mL of 100% buffer solution B for elution, and the elution flow rate was 2 mL / min.

[006...

Embodiment 3

[0070] In this embodiment, the protein powder having the activity of promoting osteoblast proliferation is prepared according to the following steps:

[0071] (1) Milk sample processing: Centrifuge fresh milk at 8000rpm for 15min to defat. Adjust the pH value of the skim milk to 4.3 with 1mol / L sulfuric acid, let it stand for 20 minutes, and then centrifuge it at 6000r / min for 20 minutes. -6°C.

[0072] (2) Purification by cation exchange chromatography: the sample was first purified by strong cation exchange chromatography SP Sepharose Big Bead (1.6×50 cm). Equilibrate with 300mL, 30mmol / L phosphate solution (buffer solution A) with a pH value of 6.8 in advance. Buffer solution B is buffer solution A containing 1mol / L NaCl. Two-stage step-by-step elution was adopted, 500 mL of a mixed solution containing 20 vol.% buffer solution B was used for elution, and then 350 mL of 100% buffer solution B was used for elution, and the elution flow rate was 2.5 mL / min.

[0073] (3) Pu...

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Abstract

The invention discloses protein meal with function of promoting bone growth and a preparation method of the protein meal. The protein meal is prepared by mixing the following raw materials by weight percent: 30wt%-60wt% of lactoferrin meal, 39wt%-69wt% of isolated whey protein meal, 0.01wt%-0.5wt% of vitamin E and 0.01wt%-0.5wt% of CaCO3. The method comprises the following steps: (1) performing cation exchange chromatography purification; (2) performing size exclusion chromatography purification; (3) mixing the prepared materials; and (4) drying a product. The obtained protein meal with the function of promoting the bone cell proliferation activity has a good effect of promoting bone formation.

Description

technical field [0001] The invention belongs to the technical field of food and relates to a protein powder and a preparation method thereof, in particular to a protein powder with the function of promoting bone growth and a preparation method thereof. Background technique [0002] Lactoferrin is a multifunctional active glycoprotein that has attracted much attention. Lactoferrin, also known as lactoferrin, mainly exists in various exocrines of mammals, such as milk, tears or saliva. It was first discovered in 1939 by Sorensen et al. when separating whey protein from animal milk. In 1953, Polis et al. Pure lactoferrin was obtained from human milk and bovine milk. Lactoferrin has been used for many years as an iron fortifier to regulate iron bioavailability. In addition, lactoferrin also has a variety of other biological functions, such as antibacterial, antiviral and antioxidant effects, participates in the immune regulation of the body, and enhances the body's ability to...

Claims

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Application Information

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IPC IPC(8): A23L1/302A23L1/304A23J1/20A23L33/15A23L33/16
CPCA23L33/16A23L33/165A23L33/19A23V2002/00A23V2200/306A23V2250/1578A23V2250/54248A23V2250/54252A23V2250/712
Inventor 杜明王聪刘猛樊凤娇宋微徐伟丽韩冰
Owner 王猛
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