High-tolerance clostridium beijerinckii and application thereof

Abstract

The invention discloses high-tolerance clostridium beijerinckii M17 with the collection number of CCTCC NO:M2013425, and an application of the high-tolerance clostridium beijerinckii M17 in production of butanol. The strain is prepared through normal temperature plasma mutagenesis and screening, has high tolerance to toxic substances in a non-detoxified wood fiber acid hydrolysis sugar solution, and can be used for producing butanol very well through fermentation in a fermentation liquor of which the phenol concentration reaches up to 2.0g / L, wherein the total solvent yield of the strain reaches 10.9g / L, the butanol yield of the strain reaches 7.9g / L, and even if the phenol concentration reaches up to 2.4g / L, the strain disclosed by the invention also has a considerable butanol production capacity, thereby showing that the strain namely clostridium beijerinckii M17 not only has an efficient utilization rate for a bagasse acid hydrolysis sugar solution, but also has high tolerance on toxin inhibitors particularly phenolic compounds in the bagasse acid hydrolysis sugar solution; the clostridium beijerinckii M17 is the excellent strain suitable for producing butanol by fermenting the bagasse.

Description

technical field [0001] The invention relates to a highly tolerant Clostridium beijerinckii capable of producing butanol by fermenting non-detoxified sugarcane bagasse acid hydrolyzed sugar solution and its application, which belongs to the technical field of biological fermentation. Background technique [0002] Butanol is a colorless liquid with a smell of alcohol, miscible with ethanol / ether and many other organic solvents; it has been widely used in the manufacture of various fine chemicals such as plasticizers. Butanol has the advantages of high energy density, can be mixed with gasoline in any ratio, can be directly used in internal combustion engines, and is convenient to transport; as a renewable new liquid fuel, it has received more and more attention. [0003] With the depletion of fossil energy and the soaring price, the use of renewable lignocellulosic raw materials (such as straw, bagasse, etc.) to ferment and produce butanol has become one of the focus and hotsp...

AI Technical Summary

Problems solved by technology

However, raw materials such as bagasse will produce inhibitors such as organic acids, furfural, and phenols after being treated with dilute acid. The removal cost of these inhibitors is relatively high and has a certain inhibitory effect on microbial growth; 2007, 97(6):1460-1469) found that inhibitors such as organic acids and furfural did not affect the fermentation of butanol, while phenolic inhibitors had a significant inhibitory effect on butanol fermentation

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