A detection reagent strip for detecting microalbumin in urine and preparation method thereof

A technology for microalbumin and detection reagents, which is applied in the field of detection reagent strips for detecting microalbumin in urine and its preparation, can solve the problems of difficult large-scale popularization and application, high production costs, and cumbersome production process of reagent strips, etc., and achieves The preparation method is simple and quick, the performance is stable, and the effects of easy visual identification and machine reading

Active Publication Date: 2016-07-06
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, immunological methods are usually used to detect microalbumin, such as immunogold standard, immunofluorescence, etc., but the production process of the reagent strips designed according to the immunological method is cumbersome, the production cost is high, and it is difficult to be popularized and applied on a large scale

Method used

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Examples

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preparation example Construction

[0023] A method for preparing a detection reagent strip for detecting microalbumin in urine, comprising the steps of:

[0024] (1) Prepare liquid A: put 40-45g of sodium citrate, 42-48g of citric acid and 30-35mL of concentrated hydrochloric acid into a container, add water to 1000mL to constant volume, and obtain a buffered aqueous solution with pH=2.0-3.5.

[0025] (2) Configure B liquid: put 40-100mL tetrahydrofuran, 5-15mL polypropylene glycol (preferably PPG2000) and 0.3-1.5g sulfonphthalein dye into the container, add methanol or ethanol to 1000mL constant volume; the purity of methanol or ethanol It is not limited, but it is preferably anhydrous methanol or anhydrous ethanol, and may also have a purity of 80% (v / v%) or lower (but anhydrous system should be used as much as possible). The sulfonphthalein dye is preferably 5',5"-dinitro-3',3"-diiodo-3,4,5,6-tetrabromophenolsulfonphthalein.

[0026] (3) Immerse the filter paper piece in the liquid A, take it out after full...

Embodiment 1

[0030] This embodiment provides a method for preparing a detection reagent strip for detecting microalbumin in urine, which includes the following steps:

[0031] (1) Prepare liquid A: put 40g of sodium citrate, 42g of citric acid and 30mL of concentrated hydrochloric acid into a container, add water to 1000mL;

[0032] (2) Prepare liquid B: put 40mL tetrahydrofuran, 5mL polypropylene glycol and 0.3g sulfonphthalein dye into the container, add methanol to 1000mL to constant volume;

[0033] (3) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 70°C to dry for 10 minutes;

[0034] (4) Immerse the dried filter paper in liquid B again, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 60°C for 5 minutes;

[0035] (5) Fixing the dried filter paper sheet with the substrate, cutting into rectangular paper strips, and making a detection reagent strip for de...

Embodiment 2

[0037] This embodiment provides a method for preparing a detection reagent strip for detecting microalbumin in urine, which includes the following steps:

[0038] (1) Prepare liquid A: put 45g of sodium citrate, 48g of citric acid and 35mL of concentrated hydrochloric acid into a container, add water to 1000mL;

[0039] (2) Prepare liquid B: put 100mL tetrahydrofuran, 15mL polypropylene glycol and 1.5g sulfonphthalein dye into the container, add ethanol to 1000mL constant volume;

[0040] (3) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 70°C to dry for 20 minutes;

[0041] (4) Immerse the dried filter paper in liquid B again, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 60°C for 15 minutes;

[0042] (5) Fixing the dried filter paper sheet with the substrate, cutting into rectangular paper strips, and making a detection reagent strip for det...

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PUM

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Abstract

The invention discloses a detection reagent strip for detecting microalbumin in urine. The detection reagent strip comprises a substrate and a filter paper piece, wherein the filter paper piece is attached on the substrate; the filter paper piece is sequentially immersed into an A solution and a B solution; the A solution is a buffered aqueous solution comprising sodium citrate, citric acid and concentrated hydrochloric acid; and the B solution is a methyl alcohol or ethanol solution comprising tetrahydrofuran, polypropylene glycol and sulfonephthalein dye. By utilizing the detection reagent strip prepared by the scheme, the sulfonephthalein dye can be quickly reacted with the microalbumin in the urine under acidic conditions, when the concentration of the microalbumin is in a range of 0-200mg / L, test paper presents obvious levels from colorlessness to mazarine, the levels are easy to visually distinguish and machine-readable, the measurement result is accurate and not disturbed by other ions in the urine, and the performance is stable. Meanwhile, a preparation method of the reagent strip is simple, convenient and rapid, the manufacturing cost is low, and the preparation method is suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to the field of urine detection, in particular to a detection reagent strip for detecting trace albumin in urine and a preparation method thereof. Background technique [0002] Microalbumin refers to the tiny amount of albumin that occurs in human urine. Albumin is a normal protein in the blood, but only very small amounts are present in the urine under physiological conditions. Under normal human metabolism, there is very little albumin in the urine, specifically less than 20mg / L per liter of urine albumin. If the microalbuminuria in the urine is found to be in the range of 20mg / L to 200mg / L after the physical examination, it belongs to microalbuminuria, and microalbuminuria reflects the abnormal leakage of protein in the human kidney. Trace protein indicators are usually used to monitor the occurrence of nephropathy. The detection of trace protein is the most sensitive and reliable diagnostic index for early detection of kidn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 白鹏利陈名利殷建尹焕才胡军
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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