Application of a kind of fermentation supernatant of mycobacillus dokdo
A technology of mycobacillus and fermentation supernatant, which is applied in the field of application of mycobacillus fermentation supernatant, can solve the problem of few biocontrol agents, and achieve good biological control effect and good control effect
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Embodiment 1
[0028] Preparation of the fermentation supernatant of 1A00493:
[0029] The applicant purchased a strain of Virgibacillus dokdonensis MCCCNO.1A00493 ("Catalogue of Chinese Marine Microorganisms", Chemical Industry Press, first edition in 2010) from the China Marine Microbial Culture Collection and Management Center; this experiment The laboratory numbered the strain as 1A00493.
[0030] 1) Streak, isolate and activate the 1A00493 strain on a 2216E medium plate to obtain a single colony;
[0031] 2) Pick a single colony and activate it in a PA bottle filled with 2216E medium;
[0032] 3) The seed liquid in the PA bottle was transferred to a 250ml Erlenmeyer flask containing 2216E medium and cultured in a liquid shake flask at 28°C for 48 hours until OD 600 =1.85;
[0033]4) The fermentation broth after shaking the flask was centrifuged at 8,000 rpm for 15 minutes to obtain the 1A00493 fermentation supernatant, which was sterilized by filtration with a 0.22 μm filter membrane...
Embodiment 2
[0035] Greenhouse experiment of 1A00493 fermentation supernatant against rice bacterial blight
[0036] 1) Rice cultivation: Soak rice seeds in 75% ethanol for 30s, then treat them with 1% mercury liter for 15min, and finally wash them with sterile water for 3 times. Use a sterilized glass plate, spread a layer of sterilized filter paper, place the treated rice seeds neatly on it, and keep it moist at 28°C. After the rice seeds germinate, they are transferred to a pot with a diameter of 20 cm and 4000 g of sterilized soil is housed, and 6 plants are planted in every pot.
[0037] 2) Pathogen and biocontrol sample preparation: rice bacterial blight pathogen (Xanthomonas oryza epv. 8 CFU / ml;
[0038] 1A00493 Greenhouse Experiment Sample Preparation The fermentation supernatant obtained in Experimental Example 1 was used.
[0039] 3) Greenhouse experiment: the method of cutting leaves is used to inoculate pathogenic bacteria. In this experiment, when the rice grows to the fift...
experiment example 1
[0046] Experimental example 1 shows that the aseptic supernatant of 1A00493 fermentation broth has a control effect of 66.7% on rice bacterial blight after 20 days of rice growth, 100% of the control with pathogenic bacteria is infected, and the control without pathogenic bacteria grows well.
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