Endogenous Bacillus amyloliquefaciens in a rice leaf and its biological preparation and application

A technology of Bacillus amyloliquefaciens and Bacillus amyloliquefaciens, applied in the field of Bacillus amyloliquefaciens and its biological preparations in rice leaves, can solve the problems of loss of disease resistance, long breeding time, environmental pollution, etc., and achieve excellent biocontrol effect , small impact on the ecological environment, and low requirements for cultivation conditions

Active Publication Date: 2022-01-14
RICE RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of a single chemical agent is likely to produce drug resistance, and the use of compound agents is prone to pesticide residues, resulting in environmental pollution and food safety problems. Although the selection of disease-resistant varieties will not have the disadvantages of chemical control, the time for breeding Long and prone to problems such as loss of disease resistance due to changes in the physiological race of Magnaporthe grisea

Method used

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  • Endogenous Bacillus amyloliquefaciens in a rice leaf and its biological preparation and application
  • Endogenous Bacillus amyloliquefaciens in a rice leaf and its biological preparation and application
  • Endogenous Bacillus amyloliquefaciens in a rice leaf and its biological preparation and application

Examples

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Embodiment 1

[0039] Example 1: Isolation, purification and preservation of endogenous Bacillus amyloliquefaciens in rice leaves

[0040] (1) Configuration of LB medium: Weigh 10 g of tryptone (Tryptone, Oxoid LTD LP0042, England), 5 g of yeast extract (Yeast extract, Oxoid LTD LP0021, England), sodium chloride (NaCl, Sinopharm Chemical Reagent Co., Ltd. Company, 10019318) 10g, add 1000mL of water and stir evenly, add 15g of agar when preparing solid medium, fully heat and dissolve, subpackage, sterilize at 121°C for 20min, and store for later use.

[0041] (2) Isolation and purification of Bacillus amyloliquefaciens in rice leaves:

[0042] The rice leaves used for the isolation of endophytic microorganisms were collected from the Baiyun Experimental Base of Guangdong Academy of Agricultural Sciences.

[0043] The isolation method of endophytic bacteria mainly refers to He Hong et al. -173.) with a slight modification, the specific operation is as follows: rinse the healthy paddy leaves ...

Embodiment 2

[0049] Embodiment 2: confrontation culture method measures the activity of Bacillus amyloliquefaciens BaYBZ

[0050] (1) The preparation of the LB medium is the same as in Example 1; the preparation of the LB liquid medium is except that agar is not added, and the other is the same as the formula of the LB solid medium, and the weighed medicament is fully dissolved and packed in conical flasks (per bottle (100mL culture solution), stoppered, bandaged, sterilized at 121°C for 20min, cooled and stored for later use.

[0051] (2) Preparation of CM medium: Weigh 6 g of Yeast extract, 6 g of enzyme-hydrolyzed casein Casaminoacid, and 10 g of sucrose in a measuring cup, set the volume to 1000 mL, fully heat and dissolve, and distribute in conical flasks (100 mL per bottle culture medium), add 1% agar powder, sterilize at 121° C. for 20 minutes, cool and store for later use.

[0052] (3) Preparation of a single colony of Bacillus amyloliquefaciens: Streak inoculation of Bacillus amy...

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Abstract

The invention discloses a rice leaf endogenous Bacillus amyloliquefaciens and its biological preparation and application, belonging to the field of biological pesticides. The name of the strain is Bacillus amyloliquefacien (Bacillus amyloliquefacien) BaYBZ, which was preserved on September 09, 2019 at the Guangdong Provincial Microbial Culture Collection Center on the 5th Floor, Building 59, Compound No. 100, Xianlie Middle Road, Guangzhou City. The deposit number is GDMCC No: 60770. The strain is derived from rice leaves and can colonize rice leaves well; it has a strong inhibitory effect on rice blast fungus and has excellent biocontrol effects on rice blast control, and the biological source is environmentally friendly and non-toxic. It can be sprayed It is applied to the surface of rice leaves for disease prevention and control, has little impact on the ecological environment, and is safer and more reliable for the consumption of rice and its processed products; the requirement for cultivation conditions is low, and it has a good development and application prospect.

Description

technical field [0001] The invention belongs to the field of biological pesticides, and in particular relates to a rice leaf endogenous bacillus amyloliquefaciens and its biological preparation and application. Background technique [0002] Rice (Oryza sativa L.) is an important food crop in my country. Nearly a quarter of the country's arable land is planted with rice, and rice accounts for about one-half of the country's total grain output. The important diseases on rice include rice blast, sheath blight, bacterial blight, bacterial streak and dwarf disease, etc., which are highly prevalent, occur in a large area, and seriously damage the yield. Among them, the rice blast caused by Pyricularia grisea (Cooke) Sacc. is one of the most important diseases in rice production. The rice blast fungus is easy to mutate and has a strong transmission ability, which generally reduces the output of rice fields by up to 20%. In severe cases, it can reach more than half. At present, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCC12N1/20A01N63/00C12R2001/07C12N1/205
Inventor 江立群孙炳蕊吕树伟张静范芝兰陈雨陈文丰潘大建张梓敬李晨
Owner RICE RES INST GUANGDONG ACADEMY OF AGRI SCI
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