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Biosensor for detecting biogenic amine and preparation method thereof

A technology of a biosensor and a manufacturing method, which is applied in the field of biosensors for detecting biogenic amines and its manufacture, can solve the problems of complex process, high cost, and long preparation time, and achieve lower phase formation temperature, strong adsorption capacity, and good compatibility sexual effect

Inactive Publication Date: 2014-07-16
GUANGDONG TESTING INST OF PROD QUALITY SUPERVISION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have good stability for the detection of biogenic amines, the preparation time is long, the process is relatively complicated, and the cost is high

Method used

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  • Biosensor for detecting biogenic amine and preparation method thereof
  • Biosensor for detecting biogenic amine and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A method for making a biosensor for detecting biogenic amines, the preparation process of which is as follows figure 2 shown, including the following steps:

[0037] 1. Preparation of a suspension dispersed with conductive metal-doped metal oxide nanoparticles.

[0038] 1) Preparation of conductive metal-doped metal oxide nanoparticles.

[0039] First, weigh 6.0 g (0.0286 mol) of citric acid monohydrate, add it into a beaker containing 200 ml of deionized water, and heat and stir at 80° C. until the citric acid is fully dissolved. Then add 5ml of concentrated nitric acid (mass fraction is about 65%, density is about 1.4g / cm3) into the solution 3 ), while accurately measuring 35ml of tetrabutyl titanate (equivalent to 0.1mol of titanium dioxide), slowly dropwise added to the solution, and kept stirring at 90°C for about 2h until the solution became clear. Keeping the temperature and stirring conditions constant, a certain amount of ammonia water was added dropwise to...

Embodiment 2

[0051] A method for making a biosensor for detecting biogenic amines, comprising the following steps:

[0052] 1. Preparation of a suspension dispersed with conductive metal-doped metal oxide nanoparticles.

[0053] 1) Preparation of conductive metal-doped metal oxide nanoparticles.

[0054] First, weigh 2.10 g (0.01 mol) of citric acid monohydrate, add it to a beaker containing 100 ml of deionized water, and heat and stir at 60° C. until the citric acid is fully dissolved. Then add 3.3ml of concentrated nitric acid (mass fraction is about 65%, density is about 1.4g / cm2) into the solution 3 ), while accurately measuring 35ml of tetrabutyl titanate (equivalent to 0.1mol of titanium dioxide), slowly dropwise added to the solution, and kept stirring at 80°C for about 2h until the solution became clear. Keeping the temperature and stirring conditions constant, a certain amount of ammonia water was added dropwise to the solution until the pH of the solution was 7.5.

[0055] Sub...

Embodiment 3

[0066] A method for making a biosensor for detecting biogenic amines, comprising the following steps:

[0067] 1. Preparation of a suspension dispersed with conductive metal-doped metal oxide nanoparticles.

[0068] 1) Preparation of conductive metal-doped metal oxide nanoparticles.

[0069]First, weigh 0.186g (0.0005mol) of disodium ethylenediaminetetraacetate (EDTA), add it into a beaker containing 11ml of deionized water, and heat and stir at 100°C until disodium ethylenediaminetetraacetate is fully dissolved. Then add 0.22ml of concentrated nitric acid into the solution, and at the same time accurately measure 35ml of tetrabutyl titanate (equivalent to 0.1mol of titanium dioxide), slowly drop into the solution, and keep stirring at 100°C for about 1 hour until the solution becomes clear . Keeping the temperature and stirring conditions constant, a certain amount of ammonia water was added dropwise to the solution until the pH of the solution was 8.0.

[0070] Subsequent...

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Abstract

The invention discloses a biosensor for detecting biogenic amine and a preparation method thereof, belonging to the technical field of sensor detection. The biosensor comprises an electrode with the surface provided with a composite film, the composite film consists of an electrochemical indicator, biogenic amine enzyme, and metallic oxide nanoparticles doped with conductive metals; the metallic oxide has semiconductor property; the mole percent of the conductive metals and the metallic oxide is 0.5-10mol%. According to the biosensor, the surface of the electrode is coated with the metallic oxide nanoparticles doped with conductive metals, the electrochemical indicator and biogenic amine enzyme; the nanoparticles are introduced to the construction of the biological sensitive interface by utilizing the surface effect, quantum size effect and dielectric confinement effect and the like of the nanoparticles; the conductive metals are doped in the metallic oxide nanoparticles; the electron transfer efficiency of the composite film is improved, redox electrochemical signals are enhanced, the sensitivity of the sensor is improved and the detection limit is lowered.

Description

technical field [0001] The invention relates to the technical field of sensor detection, in particular to a biosensor for detecting biogenic amine and a manufacturing method thereof. Background technique [0002] Biogenic amines are a general term for a class of biologically active nitrogen-containing low-molecular-weight organic compounds. It can be regarded as a substance produced by substituting 1-3 hydrogen atoms in the ammonia molecule with an alkyl or aryl group. It is an aliphatic, aliphatic or heterocyclic low-molecular-weight organic base that often exists in animals and plants. in food. Trace biogenic amines are normal active ingredients in organisms (including human body), and have important physiological functions in biological cells. However, when the human body ingests excessive biogenic amines (especially when multiple biogenic amines are ingested at the same time), it will cause allergic reactions such as headache, nausea, heart palpitations, blood pressure...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 刘付建李核梁德沛陈纪文陈卓梅王娜周桂萍谭婉琪冯艳沈宏林陈满英李衍春叶淑贞
Owner GUANGDONG TESTING INST OF PROD QUALITY SUPERVISION
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