Method for improving seedling rate of tissue culture seedlings of hylocereus undulatus britt
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A technology of tissue culture seedlings and seedling rate, which is applied in the field of plant propagation, can solve the problems of low seedling rate of pitaya tissue culture seedlings, and achieve the effects of high induction rate of adventitious buds, simultaneous proliferation and rooting, and improved seedling rate
Inactive Publication Date: 2014-07-23
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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[0004] The technical problem to be solved in the present invention is to overcome the low defect and deficiency of existing pitaya tissue culture seedling seedling rate, to improve the seedling...
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Embodiment 1
[0016] Embodiment 1 A kind of method of improving the seedling rate of dragon fruit tissue culture seedling of the present invention, its steps are as follows:
[0017] (1) Adventitious bud induction
[0018] ① Selection and treatment of explants: select semi-lignified dragon fruit stems that are free from diseases and insect pests, wash them with washing powder water first, and rinse them with tap water, then cut the stems longitudinally, remove the pith, and then cut into Stem slices with a width of 2 cm to 3 cm and a length of 3 cm to 4 cm. The outer skin of each stem slice has a complete thorn seat, and the fluff and small thorns on the thorn seat are retained.
[0019] ② Adventitious bud induction: Disinfect the treated stem slices in 75% alcohol for 30 seconds, wash them once in sterile water, transfer them to 2% sodium hypochlorite solution for disinfection for 10 minutes, and then sterilize them in sterile water. Wash 3 to 5 times, after disinfection, cut off the surf...
Embodiment 2
[0026] Embodiment 2 is the same as Embodiment 1 except that the following measures are different, and will not be repeated here.
[0027] The adventitious bud induction medium in step (1)② is: MS+TDZ 0.4mg / L+NAA0.1 mg / L+sucrose 3%+agar 0.6%, pH value is 5.8; the seedling growth medium in step (2) is : MS+6-BA 4mg / L+NAA 0.1 mg / L+activated carbon 1g / L+sucrose 3%+agar 0.6%, pH value is 5.8.
[0028] The test effect of above each embodiment:
[0029] Pitaya tissue-cultured seedlings: refers to the main stem with basal roots and / or the side branches with aerial roots that are inserted into the above-mentioned seedling medium to proliferate (that is, grow out) from adventitious buds.
[0030] These main stems with basal roots and side branches with aerial roots have roots, stems, and leaves (the small thorns of dragon fruit are its leaves, which are metamorphic leaves), and they will become complete regenerated plants and seedlings after being cut off respectively. ....
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Abstract
The invention provides a method for improving the seedling rate of tissue culture seedlings of hylocereus undulatus britt. The method comprises the following steps: carrying out adventitious bud induction, synchronous multiplication and rooting seedling culture, wherein hylocereus undulatus britt barbed seats and lignification shoots with small barbs and fluff on the barbed seats are used as explants; when lateral branches of adventitious buds grow, base roots of adventitious bud base parts grow and aerial roots of the lateral branches grow, carrying out hardening seedling; after the hardening seedling, taking out seedlings from a bottle; cutting off the lateral branches with the aerial roots and main stems with the base roots and planting in a culture medium; after the lateral branches and the main stems are survived, growing new roots; and putting the lateral branches and the main stems into pots. According to the method, the production period of the tissue culture seedlings of the hylocereus undulatus britt is short, the adventitious bud induction rate is high, the multiplication and the rooting are synchronous, the multiplication coefficient is more than 5.45 and the rooting rate is 100%; the growth vigor of multiplied seedlings is strong and the transplanting survival rate is more than 95%; one inoculated adventitious bud can grow 3-4 lateral branches with the aerial roots, namely one inoculated adventitious bud can grow 4-5 grown-up seedlings with the roots; the seedling rate of the tissue culture seedlings of the hylocereus undulatus britt is increased by 3-4 times.
Description
technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a method for improving the seedling rate of dragon fruit tissue culture seedlings. Background technique [0002] The scientific name of dragon fruit is Tianchi ( Hylocereus undatus (Haw.) Britt. & Rose.), also known as red dragon fruit, green dragon fruit, fairy honey fruit, etc., is a plant of the genus Hylocereus in the family Cactaceae. The fruit of dragon fruit is not only beautiful in appearance, but also has a health care effect. It has the effects of clearing blood, reducing fire and lowering cholesterol. Regular consumption can promote human metabolism and improve immune function. In addition, its cultivation is relatively easy, with few pests and diseases, early fruit and high yield, and can adapt to drought Climate is a new type of fruit with great potential for market development. In recent years, Yunnan, Guizhou, Guangxi, Guangdong and other pr...
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