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Preparation and application of a star-shaped bioactive filler for sulfur oxidizing bacteria immobilization based on mesh carrier

A technology of sulfur oxidizing bacteria and biological activity, which is applied in the direction of immobilization on/in organic carriers, sustainable biological treatment, biological water/sewage treatment, etc. Embedded body is easy to fall off and other problems, to achieve the effect of improving hydrophilicity, good stability, and increasing strength

Active Publication Date: 2016-01-13
湖南汨罗循环经济产业园区科技创新服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After experimental research and comparison, we found that the bioactive fillers prepared based on the traditional filler structure and embedding method were difficult to achieve the expected effect in the test application, and there were problems such as poor overall stability of the bioactive filler, easy falling off of the embedding body and water solubility, etc. many problems

Method used

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  • Preparation and application of a star-shaped bioactive filler for sulfur oxidizing bacteria immobilization based on mesh carrier
  • Preparation and application of a star-shaped bioactive filler for sulfur oxidizing bacteria immobilization based on mesh carrier
  • Preparation and application of a star-shaped bioactive filler for sulfur oxidizing bacteria immobilization based on mesh carrier

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Experimental program
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Effect test

Embodiment 1

[0033] 1. Preparation of Mesh Support

[0034] Using polypropylene high molecular polymer as the main material, adding the hydrophilic material polyvinyl alcohol (PVA) with a mass fraction of 6%, to make a sheet-shaped network carrier with a length of 1.0 cm and a width of 0.7 cm. The shape of the mesh is square, the side length is 2 mm, and the wire diameter is 1.0 mm. Six pieces of the above-mentioned sheet-shaped carriers are melted and bonded to form a star-shaped mesh carrier with an included angle of 60 degrees.

[0035] 2. Preparation of Sulfur Oxidizing Bacteria Concentrate

[0036] The sulfur-oxidizing bacteria suspension after amplification culture was centrifuged and concentrated to obtain 3.5×10 9 Individual / mL concentration of sulfur oxidizing bacteria.

[0037] 3. Preparation of embedding solution

[0038]Weigh 10kg of polyvinyl alcohol, add 50L of ultrapure water, heat to 85°C, and dissolve for 15min. Take out the dissolved polyvinyl alcohol solution and st...

Embodiment 2

[0046] 1. Preparation of Mesh Support

[0047] Using polypropylene polymer as the main material, adding polyvinyl alcohol (PVA), a hydrophilic material with a mass fraction of 6%, to make a sheet-shaped network carrier with a length of 1.0 cm and a width of 0.7 cm. The shape of the mesh is square, the side length is 2mm, and the wire diameter is 1.0mm. Six pieces of the above-mentioned sheet-shaped carriers are melted and bonded to form a star-shaped mesh carrier with an included angle of 60 degrees.

[0048] 2. Preparation of Sulfur Oxidizing Bacteria Concentrate

[0049] The sulfur-oxidizing bacteria suspension after the amplification culture was centrifuged and concentrated to obtain 3.5×10 9 Individual / mL concentration of sulfur oxidizing bacteria.

[0050] 3. Preparation of embedding solution

[0051] Weigh 10kg of polyvinyl alcohol, add 50L of ultrapure water, heat to 85°C, and dissolve for 15min. Take out the dissolved polyvinyl alcohol solution and stir it evenly,...

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Abstract

The invention discloses preparation and an application of a sulfur oxidizing bacteria immobilized star-shaped bioactive filler based on a reticulate carrier, and belongs to the technical field of water treatment. The star-shaped bioactive filler comprises two parts: an embedding body and the carrier; the carrier is a reticulate star-shaped carrier formed by taking polyethylene or polypropylene and the like as a main material, adding polyvinyl alcohol or other hydrophilic materials and carrying out hot melting or plate hot pressing; with a reticulate structure of the carrier, the embedding body can be allowed to penetrate through meshes to form a riveting fixing structure together with the carrier so as to increase the filler overall stability; an embedding liquid is formed by mixing a sulfur oxidizing bacteria concentrated solution and a polyvinyl alcohol solution; the reticulate carrier is evenly coated with the embedding liquid, and the embedding body is formed after boric acid secondary cross-linking immobilization of the embedding liquid and is combined with the reticulate carrier to obtain the sulfur oxidizing bacteria bioactive filler. The prepared bioactive filler not only can effectively solve the problems that sulfur oxidizing bacteria are poor in adhesion ability, easy to lose and the like, but also can improve the processing ability of a reactor and shorten the start-up time.

Description

technical field [0001] The invention belongs to the field of water treatment, and in particular relates to the preparation and application of a star-shaped bioactive filler for immobilizing sulfur-oxidizing bacteria based on a mesh carrier. Background technique [0002] With the rapid development of my country's industry, a large amount of sulfide-containing wastewater is often discharged in oil refining, coking, pharmaceutical and petrochemical industries. At present, the treatment of sulfide in water generally includes physical and chemical methods and biological oxidation methods, and the biological oxidation method has gradually become the sulfur-containing method due to its advantages such as no need to add chemicals, high removal efficiency, less biological sludge generation, and no secondary pollution. The main methods and research focus of wastewater treatment. When using the biological metabolic function of sulfur-oxidizing bacteria to treat sulfur-containing waste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/08C02F3/34C02F3/10
CPCY02W10/10
Inventor 杨宏陈伟胡希佳管清坤王猛鄢琳王小乐吴城锋孟婷陶慕翔尚海源赵月兰王玉洁姚仁达
Owner 湖南汨罗循环经济产业园区科技创新服务中心