Culture method of submerged photosynthetic bacteria
A technology of photosynthetic bacteria and culturing method, applied in the direction of bacteria, etc., can solve the problems of energy consumption, time-consuming, low bacterial activity, high cost, etc., and achieve the effects of good natural sedimentation performance, easy operation and low energy consumption
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[0016] Screen long rod-shaped purple non-sulfur photosynthetic bacteria: take photosynthetic bacteria activated bacteria liquid and press 10 -1 , 10 -2 , 10 -3 , 10 -4 After the gradient dilution, the four-zone streaking method was used to draw a line on the solid agar medium plate, and it was inverted on another petri dish containing a mixture of 0.5g sodium carbonate and 0.5g pyrogallic acid. The edge of the plate was sealed with paraffin wax. After 4 days of light cultivation at 30°C, red colonies will grow. Check the bacterial morphology of each colony under microscope. Pick rod-shaped colonies and separate them by streaking. Repeat this many times until they are all rod-shaped and the size is 0.6~8.0μm×1.2 under the microscope. ~2.5μm.
[0017] Use the culture medium to cultivate by two-stage culture method. In the first stage, inoculate at 20% inoculum. The culture temperature is 20-40℃, and the culture is exposed to sunlight for 3 days until pH=8.7. Use th...
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