Culture method of submerged photosynthetic bacteria

Abstract

The invention provides a culture method of submerged photosynthetic bacteria. The culture method comprises the steps of screening long rod shaped purple non-sulfur photosynthetic bacteria; culturing the purple non-sulfur photosynthetic bacteria by utilizing a culture medium and adopting a two-stage culture method: at the first stage, carrying out inoculation according to 20% inoculum size, carrying out irradiation with sunlight, culturing for three days until the pH value is 8.7, and using the culture medium which is not inoculated with the bacteria as reference bacteria liquid OD660>=2.5, wherein the culture temperature is 20-40 DEG C; at the second stage, supplementing 0.5% of fresh culture medium and continuing culturing for two days, thus obtaining a viscous submerged photosynthetic bacterium culture solution, wherein the culture medium comprises 2.0-5.0g / L of CH3COONa, 1.0-2.0g / L of (NH4)2SO4, 1.0-2.0g / L of KH2PO4, 0.2-0.8g / L of MgSO47H2O, 1.0-6.0g / L of NaCl, 1.0-3.0g / L of NaHCO3, 0.01-0.02g / L of CaCl2, 0.001-0.002g / L of FeSO4.7H2O and 0.2-1.0g / L of yeast extract. The photosynthetic bacteria can directly act on the bottoms of culture ponds so as to effectively improve the quality of water in the culture ponds and the bottom mud environments and solve the bottleneck that the photosynthetic bacteria are difficult to act on the bottom layers of water bodies due to poor settleability of the photosynthetic bacteria.

Description

[0001] technical field [0002] The invention belongs to the field of biotechnology, and in particular provides a method for cultivating submerged photosynthetic bacteria. Background technique [0003] Photosynthetic bacteria can carry out non-oxygen photosynthesis or chemoheterotrophy in different metabolic ways under different conditions such as dark aerobic and light anaerobic, and have strong transformation and removal of ammonia nitrogen, nitrite nitrogen, sulfur At the same time, photosynthetic bacteria cells are rich in protein with comprehensive amino acid types, coenzyme Q and B vitamins and other physiologically active substances, so photosynthetic bacteria are widely used as water purifiers or feed additives in aquatic products. Aquaculture. [0004] Photosynthetic bacteria are used as water purifiers in aquaculture ponds. Due to their poor settling properties, photosynthetic bacteria cannot naturally settle to the bottom of the water body, so they cannot directl...

AI Technical Summary

Problems solved by technology

Such as the above methods such as spraying with external equipment or immobilizing photosynthetic bacteria, although the settlement problem of photosynthetic bacteria has been improved to a certain extent, they all have the disadvantage of energy consumption and time-consuming. The former requires specific equipment and high investment costs. The latter is difficult to ensure the activity of the bacteria, which is not conducive to the promotion and use of large areas.

If a kind of photosynthetic bacteria agent that can settle naturally can be developed, it can effectively solve the problems of high cost, difficult operation and low bacterial activity of the current settled photosynthetic bacteria, effectively improve the water quality of aquaculture water, and improve the efficiency of aquaculture

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