Quantitative determination method of immune chromatography test strip
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A quantitative detection method and the technology of immunochromatographic test paper, which are applied in the field of dry chemical detection, can solve the problems of increasing the labor complexity of clinical testing staff, and achieve the effect of increasing the linear detection range and improving accuracy
Inactive Publication Date: 2014-07-30
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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In clinical applications, operators have to manually dilute,
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Embodiment 1
[0048] A method for quantitatively detecting C-reactive protein is provided, comprising the steps of:
[0049] 1. Structural design of immunochromatographic test strips
[0050] Four protein bands are immobilized on the reaction membrane of the test strip, which are an internal reference line immobilized with 0.2 μg rabbit IgG, the first detection line immobilized with 0.4 μg CRP monoclonal antibody a, and immobilized with 0.4 μg CRP monoclonal antibody a. A second test line for antibody a, an excess antigen test line immobilized with 0.5 μg goat anti-mouse IgG.
[0051] 2. Detection buffer design
[0052] The detection buffer contained 0.2 μg of cy5 fluorescently labeled goat anti-rabbit IgG and 1 μg of cy5-labeled mouse CRP monoclonal antibody.
[0053] 3. Quantitative detection algorithm of test strips
[0054] Take 10 serum samples of standard concentration, add them to the detection buffer, add them dropwise to the test strips and react for a period of time, and cooper...
Embodiment 2
[0062] A quantitative detection method for rapid detection of parathion-methyl is provided, comprising the steps of:
[0063] 1. Structural design of the test strip
[0064] Four bands are fixed on the reaction membrane of the test strip, which are an internal reference line immobilized with 1 μg rabbit IgG, the first detection line immobilized with 0.5 μg methyl parathion artificial antigen, and immobilized with 0.5 μg methyl parathion A second test line for phospho-artificial antigen, an excess antigen test line immobilized with 0.5 μg goat anti-mouse IgG.
[0065] 2. Detection buffer design
[0066] The detection buffer contained 1 μg of cy5 fluorescently labeled goat anti-rabbit IgG and 1 μg of cy5-labeled mouse methyl parathion monoclonal antibody.
[0067] 3. Quantitative detection algorithm of test strips
[0068] Take 7 standard concentrations of methyl parathion solution, add it to the detection buffer, drop it on the test strip and react for a period of time, and ...
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Abstract
The invention discloses a quantitative determination method of an immune chromatography test strip. The quantitative determination method comprises the following steps: drawing a standard curve; dropwise adding a liquid to be detected to the immune chromatography test strip which comprises an internal reference line, at least one detection line and an excessive antigen detection line, detecting, and reading a detection signal capable of reflecting the intensity of antigen-antibody reaction to obtain a reading result; carrying out linear regression on the reading result of the nth detection line by utilizing the nth standard curve when the detection signals of the n-1 detection lines of at least one detection line exceed a threshold value according to the reading results, and calculating to obtain the concentration of the liquid to be detected. By adopting the mode, the quantitative detection method disclosed by the invention can solve the problem that the low-concentration measurement and the high-concentration measurement are simultaneously carried out, and can be used for the quantitative detection on biological macromolecules or micromolecules, such as serum markers, microbe antigens, virus particles and illicit drugs; the linear detection range of the test strip is enlarged, the detection accuracy is improved, and the accurate quantitative detection on a target detection object is realized.
Description
technical field [0001] The invention relates to the field of dry chemical detection, in particular to a quantitative detection method of an immunochromatographic test strip. Background technique [0002] Immunochromatography uses a nitrocellulose membrane as a support for reactants, and uses the pore effect of the membrane to react. The technology can be divided into vertical diffusion and horizontal diffusion. The dot gold hybridization method of vertical diffusion was born in the early stage, but it was not popularized and used as a mainstream reagent because of its complicated operation. With the development of membrane technology, lateral flow and lateral diffusion test strips have shown good advantages and have been greatly promoted. The most widely used colloidal gold rapid diagnostic test paper in lateral flow immunochromatography is used in the detection of early pregnancy (HCG). Due to its convenience, speed, and high specificity, it is especially suitable for imm...
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IPC IPC(8): G01N33/558
CPCG01N33/54373
Inventor 尹焕才
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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