Method for preparing tribonema biological oil and tribonema biological oil prepared by method

Abstract

The invention related to a method for preparing tribonema biological oil, the tribonema biological oil prepared through the method and biodiesel, aviation kerosene, food or feed prepared from the tribonema biological oil. Particularly, one or two of isomorphous tribonema, common tribonema, small-size tribonema, pseudo-silk tribonema and cystic tribonema or a mixture of more algae is subjected to scale culture on an open culture device or a closed culture device or a culture device on which the tribonema grows in a biofilm form and an attaching manner to obtain oil-containing tribonema biomass. The tribonema biomass is extracted with an organic solvent, or squeezed or prepared by adopting an extracting and squeezing combined method to obtain biological oil containing 40-80wt% of triglyceride, 65-85wt% of hexadecenoic fatty acid in the fatty acid and 6-15wt% of eicosatetraenoic polyunsaturated fatty acid.

Description

technical field [0001] The present invention relates to a method for preparing bio-oil from Xanthophyllum, Zanthrocystis bio-oil prepared therefrom, and biodiesel, aviation fuel, food, food additives, feed or bait produced from the Zanthylacia bio-oil . The invention also relates to a method for industrially cultivating the yellow hair algae. Background technique [0002] In recent years, fossil fuels have become increasingly scarce, and the energy crisis has become an issue that has attracted the attention of the government and academic circles. In addition, the proliferation of fossil fuels has led to increased air pollution, affecting the atmospheric environment and human health. Therefore, renewable, carbon-neutral, and widely sourced bioliquid fuels have attracted much attention. Among them, two types of liquid fuels, biodiesel and bioaviation fuel, are particularly important. Biodiesel is an oxygen-containing biofuel obtained from bio-oil through methyl esterificat...

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Problems solved by technology

[0019] In the first aspect, the present invention provides an industrialized method for cultivating yellow-haired algae with high oil content in view of the shortcomings of insufficient production capacity of biodiesel raw materials, difficulty in harvesting unicellular algae in industrial production, and easy production reduction due to ingestion by insect pests , the method comprises: selecting algal strains of the genus Xanthophyllum as the algae species, and cultivating the seed solution in the whole BG11 culture solution; subsequently inoculating the Xanthophyllium seed solution and the nutrient-limited medium together into an enlarged culture device, and then The light intensity is 50~1000μmol / m 2 / s indoor artificial light, or 50~2000μmol / m 2 / s Under outdoor natural light, the culture temperature is 15°C to 35°C, and the gas containing 1% to 15% carbon dioxide is introduced into the culture for 5 days to 25 days; The concentration of sodium nitrate in the medium is below 15g / L, for example, the concentration of sodium nitrate is 0.15g / L~0.75g / L, 0.75g / L~1.5g / L, 1.5g / L~15g / L

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