Method for determining sodium copper chlorophyllin in olive oil

Abstract

The invention discloses a method for determining sodium copper chlorophyllin in olive oil. A sample is extracted through an extracting solution and then is subjected to low-temperature high-speed centrifugation, and supernatant is separated through a liquid chromatography, is detected through a diode array detector, is subjected to nature determination through retention time and is quantified by an external standard method. The method comprises the following steps: step1. preparing a standard working solution, a standard using solution for standard addition and olive oil mixed with sodium copper chlorophyllin in a simulating manner; step 2. respectively carrying out liquid chromatogram determination on the three kinds of solutions of the step 1 by adopting a high performance liquid chromatography; drawing a regression curve of the standard working solution by using the concentration x of sodium copper chlorophyllin in the standard working solution as an x-coordinate and the correspondingly-measured peak area y as a y-coordinate, and calculating a slope factor a and an intercept b of the regression curve according to a linear equation (y=ax+b) obtained based on the curve; step 3. according to the standard curve equation, calculating the concentration of sodium copper chlorophyllin in olive oil. According to the method, a universal liquid chromatographic column and an ultraviolet detector are adopted to carry out high performance liquid chromatogram determination for the sodium copper chlorophyllin, and the interference of determination of other substances is eliminated. According to the method, the content of sodium copper chlorophyllin in olive oil can be accurately measured, the quantifying is accurate and reliable and the reproducibility is good.

Description

technical field [0001] The invention relates to a method for measuring sodium copper chlorophyllin in olive oil. Background technique [0002] With its unique physical and chemical indicators and health functions, olive oil has gradually become an ideal edible oil in the new century. It is rich in monounsaturated fatty acids, namely oleic acid, linoleic acid, and linolenic acid, as well as vitamin A, vitamin B, and vitamin D. , vitamin E, vitamin K and antioxidants, etc., are considered to be the most suitable oils for human nutrition among the oils found so far. [0003] According to a report released by the U.S. Department of Agriculture in September 2013, from October 2012 to September 2013, the average price of sunflower oil in the U.S. market was about 9,000 yuan per ton; the average price of soybean oil was about 9,000 yuan per ton. 6,500 yuan; while the price of authentic extra virgin olive oil is 32,000-36,000 yuan per ton. Even if the cost of sunflower oil is high...

AI Technical Summary

Problems solved by technology

[0005] At present, there is no detection method and standard for the content of sodium copper chlorophyllin, so it is urgent to formulate a standard detection method for sodium copper chlorophyllin in olive oil to fill the gap at home and abroad, so as to strengthen the inspection and supervision of olive oil in the market

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