Pharmaceutical composition with myocardial ischemia resisting effect and preparation method and application thereof
An anti-myocardial ischemia and drug technology, applied in drug combinations, medical preparations containing active ingredients, applications, etc., can solve problems such as no research reports on myocardial ischemia
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Embodiment 1
[0058] Example 1: Preparation process of anti-myocardial ischemia traditional Chinese medicine drug substance combination
[0059] Ginseng 300g Poria 300g
[0060] Polygala 200g Puhuang 200g
[0061] According to the above ratio, take 1 kg of raw material drug decoction pieces, extract 3 times with 6 times the amount of 50% ethanol under reflux, and extract for 1.5 hours each time, combine the two extracts (total 20L) and concentrate under reduced pressure until the relative density is 1.15-1.20g / ml Add water to disperse the clear paste, so that the concentration of the sample solution is 0.05g / mL (in terms of the amount of raw medicinal materials), and the sample solution is adsorbed by the DM130 type macroporous adsorption resin, and the ratio of sample volume to resin volume is 0.25g : 1mL, the ratio of diameter to height of the resin column is 1:8.7, and the adsorption flow rate is 1bv / h; Elute 4 times the resin volume with ethanol, the elution flow rate is 2bv / h, collec...
Embodiment 2
[0088] Ginseng 300g Poria 300g
[0089] Polygala 200g Puhuang 200g
[0090] According to the above ratio, take 1 kg of raw material drug decoction pieces, extract 3 times with 6 times the amount of 50% ethanol under reflux, and extract for 1.5 hours each time, combine the two extracts (total 20L) and concentrate under reduced pressure until the relative density is 1.15-1.20g / ml Add water to disperse the clear paste, so that the concentration of the sample solution is 0.05g / mL (in terms of the amount of raw medicinal materials), and the sample solution is adsorbed by the DM130 type macroporous adsorption resin, and the ratio of sample volume to resin volume is 0.25g : 1mL, the ratio of diameter to height of the resin column is 1:8.7, and the adsorption flow rate is 1bv / h; Elute 4 times the resin volume with ethanol, the elution flow rate is 2bv / h, collect 40% ethanol eluate, recover the solvent, elute 3 times the resin volume with 90% ethanol, the elution flow rate is 2bv / h, c...
Embodiment 3
[0117] Ginseng 300g Poria 300g
[0118] Polygala 200g Puhuang 200g
[0119] According to the above ratio, take 1 kg of raw material drug decoction pieces, extract 3 times with 6 times the amount of 50% ethanol under reflux, and extract for 1.5 hours each time, combine the two extracts (total 20L) and concentrate under reduced pressure until the relative density is 1.15-1.20g / ml Add water to disperse the clear paste, so that the concentration of the sample solution is 0.05g / mL (in terms of the amount of raw medicinal materials), and the sample solution is adsorbed by the DM130 type macroporous adsorption resin, and the ratio of sample volume to resin volume is 0.25g : 1mL, the ratio of diameter to height of the resin column is 1:8.7, and the adsorption flow rate is 1bv / h; Elute 4 times the resin volume with ethanol, the elution flow rate is 2bv / h, collect 40% ethanol eluate, recover the solvent, elute 3 times the resin volume with 90% ethanol, the elution flow rate is 2bv / h, c...
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