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Enclosed formaldehyde virus-inactivating pipeline system and applications thereof

A pipeline system and formaldehyde inactivation technology, which is applied in the field of closed formaldehyde inactivation virus pipeline system, can solve the problems of increasing the operation steps of technicians, increasing the degree of pollution risk, and the need for repeated assembly, so as to avoid external pollution and save energy. Production cost, the effect of ensuring sealing

Active Publication Date: 2014-08-20
SINOVAC BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the inactivation operation environment, part of the operation is open production. The pipelines used are relatively simple and scattered, requiring repeated assembly, increasing the operation steps of technicians, and greatly increasing the risk of pollution.
In the process of pipe cleaning, repeated disassembly and assembly are required, and pipes for different purposes are easy to confuse

Method used

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  • Enclosed formaldehyde virus-inactivating pipeline system and applications thereof
  • Enclosed formaldehyde virus-inactivating pipeline system and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Closed formaldehyde inactivation virus pipeline system

[0033] The closed formaldehyde inactivating virus pipeline system provided in this embodiment has a schematic structural diagram such as figure 1 with 2 Shown. The pipeline system includes virus liquid tank 1, pipeline L116, formaldehyde solution tank 2, pipeline L217, T tee 14, pipeline L318, peristaltic pump 4, pipeline L419, sterilizing filter 5, pipeline L520, inactivation tank 3, Balance 6 and magnetic stirrer 7;

[0034] The pipe L116 extends into the barrel through the lid 11 of the virus liquid barrel to ensure that one side of the pipe L1 is in contact with the barrel bottom. The lid 11 is connected with an air filter 8, and the other end of the pipe L116 passes through the pipe L217 and the pipe L318. Tee 14-phase connection;

[0035] The pipe L217 extends into the barrel through the lid 12 of the formaldehyde solution barrel to ensure that one side port of the pipe L2 is in contact with the bottom ...

Embodiment 2

[0047] Example 2 Using closed formaldehyde inactivation virus pipeline system to inactivate hepatitis A virus liquid

[0048] The hepatitis A virus liquid was inactivated by using the formaldehyde inactivation virus pipeline system in Example 1. The details of the intermediate control and aseptic production operation methods during the inactivation operation are as follows:

[0049] 1. After culturing human diploid cells in a cell factory, inoculating hepatitis A virus, add cell lysate to harvest hepatitis A virus solution.

[0050] 2. The hepatitis A virus harvest liquid is purified and refined to obtain a relatively pure hepatitis A virus liquid.

[0051] 3. Inactivate hepatitis A virus liquid to produce inactivated hepatitis A virus liquid.

[0052] (1) After cleaning and disinfecting the inactivation room, connect the combined pipeline.

[0053] (2) Hepatitis A virus liquid filtration ( figure 1 ): Connect virus liquid tank 1 figure 1 Middle L1 pipeline, and 15 hemostatic forceps fi...

Embodiment 3

[0056] Example 3 Use of closed formaldehyde inactivation virus pipeline system to inactivate polio virus liquid

[0057] The polio virus liquid was inactivated using the formaldehyde inactivation virus pipeline system in Example 1. The details of the intermediate control and aseptic production operation methods during the inactivation operation are as follows:

[0058] 1. After resuscitation and expansion of human diploid cells, the polio virus is harvested after inoculation with polio virus seeds.

[0059] 2. The polio virus harvest liquid is purified and refined to obtain a relatively pure polio virus liquid.

[0060] 3. Inactivate polio virus liquid to produce inactivated polio virus liquid.

[0061] (1) After cleaning and disinfecting the inactivation room, connect the combined pipeline.

[0062] (2) Polio virus fluid filtration ( figure 1 ): Connect virus liquid tank 1 figure 1 Middle L1 pipeline, and 15 hemostatic forceps figure 1 The middle point ② is clamped at the pipe, and the...

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Abstract

The invention provides an enclosed formaldehyde virus-inactivating pipeline system. The system comprises a virus liquid barrel (1), a pipeline L1 (16), a formaldehyde solution barrel (2), a pipeline L2 (17), a T-joint (14), a pipeline L3 (18), a peristaltic pump (4), a pipeline L4 (19), a sterilization filter (5), a pipeline L5 (20), an inactivating barrel (3), an electronic balance (6), and a magnetic stirrer (7). The system solves the problems of low formaldehyde virus inactivation efficiency and pollution hazards existing during the aseptic production process due to the diffused pipelines and repeated operation, improves the inactivation efficiency during the virus inactivation process and the control level of aseptic operation, and increases the qualified rate of vaccines.

Description

Technical field [0001] The invention relates to a virus inactivation process, in particular to a closed formaldehyde inactivation virus pipeline system and its application. Background technique [0002] In the inactivation process of vaccine production, after the purified virus liquid is sterilized and filtered, a certain concentration of formaldehyde solution needs to be injected to inactivate it to obtain an antigen with stable protein structure and high immunogenicity for preparing the final vaccine. product. In the virus inactivation process, using a certain concentration of formaldehyde solution to inactivate the virus is the most common method. During the implementation of this method, the main factors that have a greater influence on the immobilization of antigen surface proteins by formaldehyde are: the addition of the formaldehyde solution, the flow rate and the stirring of the virus liquid. Adding too little or too much formaldehyde solution to the virus solution can ...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/04C12M1/02C12N7/06
CPCC12M27/02C12M37/00
Inventor 张健董兴华张颖黄金凤李静尹卫东
Owner SINOVAC BIOTECH
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