Ginger extract for the protection of stem cells
A ginger extract and stem cell technology, applied in the new ginger extract field, can solve the problems of abnormal pigmentation, disturb the melanin stem cell group, etc., and achieve the effect of preventing aging
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Embodiment 1
[0316] Freeze fresh ginger root at -18°C, then shred and cut ginger root. The material was dried at 30°C for 17 hours using a continuous flow dryer. At 35°C, 250bar, 10kgCO 2 The dried material was supercritically extracted with carbon dioxide at a flow rate of / h*kg raw material. Due to the high content of essential oils, the first fraction collected during the first three hours of the extraction process was discarded. The second fraction from the 3rd hour to the 5th hour after the start of the reduced pressure treatment was collected to obtain the ginger extract according to the present invention. 32 kg of dried ginger root yielded 1 kg of ginger extract according to the invention.
[0317] The composition of the extract thus obtained is shown in Table 1. figure 1 The HPLC chromatogram of the extract is shown.
[0318] Table 1:
[0319] Composition of ginger extract according to the invention (content % b.w.)
[0320] Element
Embodiment 2
[0322] Stem Cell Protection (In Vitro)
[0323] HHFSC (human hair follicle stem cells) isolated from hair follicle bulge (Celprogen) were cultured in a dish precoated with human hair follicle stem cell extracellular matrix. Cells were incubated with test compound for 2 hours, then UVB-irradiated, and cells were incubated with test compound for 16 hours. Cells were irradiated with 25 mJ / cm2 UVB in the presence of buffer. Induction of apoptosis was assessed by Caspase 3 / 7 protein expression (Caspase-Glo3 / 7, Promega) and quantified by chemiluminescent detection.
[0324] The inhibition of apoptosis induction in the presence of the test substance is calculated according to the following equation:
[0325]
[0326] The abbreviations have the following meanings:
[0327] RLU test substance:
[0328] RLU containing test substance and wells with UVB irradiation
[0329] RLU control:
[0330] RLU of wells without test substance but with UVB irradiation
[0331] RLU control wi...
Embodiment 3
[0340] Cyclooxygenase-2 (COX-2) is mixed with the fluorescent substrate 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) and heme in the presence of the test substance. The reaction was started by adding the substrate arachidonic acid.
[0341] COX-2 converts arachidonic acid to prostaglandin endoperoxide G2 (PGG2). Reduction of PGG2 to the corresponding alcohol PGH2. In this reaction, ADHP produces the fluorescent resorufin. Resorufin was quantified under the condition of extinction wavelength of 535nm and excitation wavelength of 590nm.
[0342] The inhibition rate of COX-2 activity in the presence of the test substance was calculated according to the following equation:
[0343]
[0344] The abbreviations have the following meanings:
[0345] Resorufin test substance:
[0346] Concentration of resorufin in wells containing test substance and COX-2
[0347] Resorufin control:
[0348] Concentration of resorufin in wells without test substa...
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