In-vitro cytology experiment device with multi-angle ultrasonic loading and method

Abstract

The invention discloses an in-vitro cytology experiment device with multi-angle ultrasonic loading, and a method. The device comprises an ultrasonic generator and at least one ultrasonic loading device which is connected with the ultrasonic generator, wherein each ultrasonic loading device comprises a shell and a plurality of concave rotating screw rods; each concave rotating screw rod comprises a rotating head, a main long rod, a first connecting rod and a second connecting rod; a plurality of ultrasonic transmitting probes are arranged on the main long rod; the plurality of ultrasonic transmitting probes on each concave rotating screw rod are formed into ultrasonic transmitting probes arranged in an array mode; and when a cell culture porous plate is fixed at the upper part of the shell, each ultrasonic transmitting probe can be aligned to each hole center of the cell culture porous plate in any angle. By adopting the device and the method, the influence of ultrasonic action in different incidence angles to experiments of single cell culture or co-culture of biologic materials and cells can be studied, a plurality of groups of experiments can be made simultaneously, and the efficiency of the experiment process is improved.

Description

technical field [0001] The invention relates to a cell biology experiment device, in particular to a multi-angle ultrasonic loading in vitro cytology experiment device and method, belonging to the field of medical devices. Background technique [0002] Ultrasound is widely used in medicine. Ultrasound can be used to remove stones, remove diseased tissues (such as cataracts), and remove bone cement in joint replacement revisions; in clinical applications, it can generate heat in tissues to relieve pain, relieve muscle spasms, and improve joints. and muscle activity; low-intensity ultrasound devices are used for medical imaging, imaging vital organs, fetuses, eyeballs, and bone tissue in the body; studies have also shown that low-intensity ultrasound can be used to promote fracture healing. [0003] Ultrasound is a kind of sound energy beyond the range of human hearing. Its propagation in the body can cause changes in the function and metabolism of tissues, cells or biomolecu...

AI Technical Summary

Problems solved by technology

In summary, as an exogenous physical stimulus, the loading of ultrasound has a close influence on the metabolism of cells, enzymes, and biomolecules in biological tissues. However, in vitro cytology experiments using ultrasound to extensively study various biological materials are still insufficient. The underlying mechanism of ultrasound action is still unclear

[0004] At present, the experimental devices used to study the effects of ultrasonic loading on cell proliferation, adhesion, and differentiation during cell culture are mainly a single device that conducts ultrasonic waves through a liquid in a petri dish or a closed chamber. There are defects such as cumbersome operation, not conducive to repeated experiments in batches, and the experimental environment is difficult to meet the requirements of cell culture.

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