Unlock instant, AI-driven research and patent intelligence for your innovation.

Counting method for gold nanomaterial on transparent levelled sheet-shaped substrate surface

A technology of substrate surface and counting method, which is applied in the field of image processing, can solve the problems of increased experimental steps and complexity, low sensitivity of optical signal response of gold nanomaterials, and inability to realize quantification of single nanomaterials, etc.

Inactive Publication Date: 2014-08-27
PEKING UNIV
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Affected by the weak scattered light intensity of a single gold nanoparticle and the stray light on the substrate surface, the existing methods are not sensitive to the optical signal of gold nanomaterials, and cannot quantify a single nanomaterial, and subsequent treatments such as silver staining are often required to amplify The signal of gold nanomaterials, so that the detection limit of the corresponding analytical method is limited, and the experimental steps and complexity increase

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Counting method for gold nanomaterial on transparent levelled sheet-shaped substrate surface
  • Counting method for gold nanomaterial on transparent levelled sheet-shaped substrate surface
  • Counting method for gold nanomaterial on transparent levelled sheet-shaped substrate surface

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Example 1: The imaging time of the background scattering image, the characteristic scattering image of gold nanomaterials and the scattering image of the sample to be tested is 620 ms, and the actual size corresponding to each pixel in the image is 0.15 μm×0.15 μm, and the size of a single image is Both are 4140×3096 pixels. Using the counting method of the present invention to measure the amount of gold nanoparticles adsorbed on the surface of the aminated glass slide, it specifically includes the following steps:

[0110] 1) Add 5 μL of deionized water dropwise to the surface of a microscope slide, cover with an aminated microscope cover glass, observe under a dark-field microscope, and collect background scattering images.

[0111]2) The spherical gold nanoparticle sol with a particle size of 50 nm was dropped onto the surface of the aminated microscope cover glass, and after standing for 5 minutes, the microscope cover glass was rinsed with deionized water and dried...

Embodiment 2

[0143] Example 2: The imaging time of the background scattering image, the characteristic scattering image of gold nanomaterials and the scattering image of the sample to be tested is 620ms, and the actual size corresponding to each pixel in the image is 0.15 μm×0.15 μm, and the size of a single image is Both are 4140×3096 pixels. Using the counting method of the present invention to determine the amount of gold nanoparticles attached to the surface of the glass slide modified by the DNA aptamer in the detection system of the DNA aptamer-thrombin sandwich method, it specifically includes the following steps:

[0144] 1) Add 5 μL of deionized water dropwise to the surface of a microscope slide, cover with a microscope cover glass with a surface-modified thrombin aptamer, observe under a dark-field microscope, and collect a background scattering image.

[0145] 2) Add 50 μL of the solution containing the thrombin sample dropwise to the surface of the microscope coverslip modifie...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a counting method for a gold nanomaterial on a transparent levelled sheet-shaped substrate surface. The counting method comprises the following steps: adding a solvent used in sample determination to the transparent levelled sheet-shaped substrate surface, then placing under a dark-field microscope, observing, and acquiring to obtain a background scattering image; adding the gold nanomaterial to the transparent levelled sheet-shaped substrate surface, then placing under the dark-field microscope, observing, and acquiring to obtain a characteristic scattering image of the gold nanomaterial; respectively adding to-be-detected samples containing different concentrations of the gold nanomaterial to the transparent levelled sheet-shaped substrate surface, then placing under the dark-field microscope, observing, and acquiring to obtain a plurality of scattering images of the to-be-detected samples; and successively carrying out image analysis on the background scattering image, the characteristic scattering image of the gold nanomaterial and the scattering images of the to-be-detected samples, and thus obtaining the number of the gold nanomaterial in the scattering images of the to-be-detected samples. The counting method can be widely applied in counting of the gold nanomaterial on various transparent levelled substrates and having various structures and sizes.

Description

technical field [0001] The invention relates to the field of image processing, in particular to a method for counting gold nanomaterials on the surface of a transparent flat sheet substrate. Background technique [0002] At present, gold nanomaterials such as spherical nanoparticles and nanorods have been widely used in chemical and biological analysis and detection. Due to the surface plasmon resonance properties of gold nanomaterials, its scattering spectrum has strong and adjustable characteristic scattering peaks, so gold nanomaterials can be used for light emission on the surface of transparent flat sheet substrates (such as glass, plexiglass, plastic, etc.) Scattering biochemical assay detection. The existing biochemical analysis and detection method adopts the principle of direct binding or sandwich method, in which gold nanomaterials modified with recognition molecules are adsorbed or bonded to the surface of the substrate, and then the analyte is qualitatively and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/47G06T7/00
Inventor 许潇李娜李甜徐重行刘锋
Owner PEKING UNIV