Recycling of Yeast Wastewater to Produce Multifunctional Biological Organic Fertilizer
A bio-organic fertilizer and yeast wastewater technology, applied in the direction of organic fertilizers, to achieve low production costs, improve farmland environmental quality, and promote absorption and utilization
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Embodiment 1
[0019] Embodiment 1: Yeast waste water produces bacillus licheniformis bio-organic fertilizer
[0020] Yeast wastewater pretreatment: adjust the pH of the wastewater to about 7.5 with plant ash rich in potassium carbonate from a biomass thermal power plant, and sterilize at 115°C for 15 minutes.
[0021] Preparation of liquid seeds: Preparation of liquid seeds: inoculate Bacillus licheniformis preserved on a slant into a triangular flask containing pretreated yeast wastewater, and vibrate at 35° C. for 24 hours at 150 rpm to obtain liquid seeds.
[0022] Liquid fermentation: Inoculate liquid seeds with a volume ratio of 5% in a fermenter equipped with pretreated yeast wastewater, and culture at 35°C with sterile air for 20 hours; the cultured fermentation broth is counted by plate bacteria, and the number of viable bacteria up to 9×10 8 CFU / ml or more, the obtained fermentation broth can be used after dilution as a liquid bacterial agent.
[0023] Preparation of bio-organic ...
Embodiment 2
[0024] Embodiment 2: Bacillus licheniformis bio-organic fertilizer is used to prevent cucumber damping-off
[0025]Cucumber damping-off is a disease caused by fungal infection. The soil in the greenhouse where cucumbers have been planted for many years is collected, and inoculated with Pythium melon fruit ( Pythium aphanidermatum ), mix them evenly, put them into flowerpots, set up 2 groups of flowerpots, 10 pots in each group, and keep higher humidity and lower light intensity in the greenhouse at 15°C to 20°C. The first group of flower pots were directly sown with cucumber seeds, 15 seeds per pot, as a control. Before sowing cucumber seeds, group 2 mixed 0.3% Bacillus licheniformis biocontrol agent into the soil by weight, and then sowed 15 seeds per pot. The total number of seedlings was counted after unearthed, and the total number of lodging seedlings was counted after the seedlings grew 1 true leaf and before 2 true leaves. The results showed that the lodging rate of...
Embodiment 3
[0026] Embodiment 3: Bacillus licheniformis bio-organic fertilizer is used to increase the output of vegetables and improve the quality of vegetables
[0027] Using the vegetable greenhouse plot experiment, set up 8 experimental plots with exactly the same conditions, each plot 60m 2 , 2 plots are a group of parallel experiments, divided into 4 groups, planting small rapeseed, in the seedling stage of small rapeseed, the 2 plots of the first group were not fertilized as a control; the 2 plots of the second group were each applied with 1×10 7 100 kg of CFU / gram of Bacillus licheniformis bio-organic fertilizer was used as the base fertilizer, and 1×10 7 The dilution of the Bacillus licheniformis fermentation liquid of CFU / ml; The 2 plots of the 3rd group apply urea once respectively after the rape germinates completely and after 3 weeks according to the fertilization rate of 15 kg / mu; The amount of fertilization per mu was applied urea once after the rape fully germinated and t...
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