Establishment method for echinogorgia pseudossapo laser-Raman spectrum
A technology of laser Raman spectroscopy and establishment method, which is applied in the field of qualitative identification of gorgonian sparse branches, can solve the problems of undiscovered gorgonian laser Raman research reports, etc., and achieves the effects of rapid and accurate qualitative analysis and easy operation.
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[0011] The present invention will be described in further detail below in conjunction with the examples, but the present invention is not limited to the following implementation.
[0012] After cutting 0.10 g of the sample of Prunus lenticus into strips and chunks, it was put into a sample bottle for quick freezing, and the end point temperature was about -80°C. Transfer the quick-frozen sample to the drying chamber of the vacuum freeze dryer, the vacuum degree is 0.08MPa, and the drying time is 36h. The dried gorgonian sample was ground into powder with an agate mortar, and then filtered with a 100-mesh filter to obtain a powdered sample of gorgonian.
[0013] A laser beam with a wavelength of 780nm was filtered and then introduced into a Nikon inverted biological microscope (TE2000U, Nikon). After the laser beam is focused by an oil immersion objective lens (aperture 1.30, magnification 100 times), a single-beam optical potential well is formed near the focal point. The sa...
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