Establishment method for echinogorgia pseudossapo laser-Raman spectrum

A technology of laser Raman spectroscopy and establishment method, which is applied in the field of qualitative identification of gorgonian sparse branches, can solve the problems of undiscovered gorgonian laser Raman research reports, etc., and achieves the effects of rapid and accurate qualitative analysis and easy operation.

Inactive Publication Date: 2014-09-03
GUANGXI ACAD OF SCI
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Problems solved by technology

At present, no laser Raman research reports related to gorgonians have been found

Method used

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  • Establishment method for echinogorgia pseudossapo laser-Raman spectrum

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Embodiment Construction

[0011] The present invention will be described in further detail below in conjunction with the examples, but the present invention is not limited to the following implementation.

[0012] After cutting 0.10 g of the sample of Prunus lenticus into strips and chunks, it was put into a sample bottle for quick freezing, and the end point temperature was about -80°C. Transfer the quick-frozen sample to the drying chamber of the vacuum freeze dryer, the vacuum degree is 0.08MPa, and the drying time is 36h. The dried gorgonian sample was ground into powder with an agate mortar, and then filtered with a 100-mesh filter to obtain a powdered sample of gorgonian.

[0013] A laser beam with a wavelength of 780nm was filtered and then introduced into a Nikon inverted biological microscope (TE2000U, Nikon). After the laser beam is focused by an oil immersion objective lens (aperture 1.30, magnification 100 times), a single-beam optical potential well is formed near the focal point. The sa...

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Abstract

The invention relates to an establishment method for an echinogorgia pseudossapo laser-Raman spectrum. The establishment method for the echinogorgia pseudossapo laser-Raman spectrum comprises the following steps: (1) preparing a test sample: taking an echinogorgia pseudossapo sample and cutting into strip-shaped grains, filling the grains into a sample bottle to be quickly frozen, drying the quickly-frozen sample by using a vacuum freeze drying machine and grinding the sample into powder, and filtering the powder by using a screening net to obtain a powdery echinogorgia pseudossapo sample; (b) putting a buffering solution containing the particle-shaped echinogorgia pseudossapo into a focal point by using a focusing laser beam, and exciting Raman scattering of the caught echinogorgia pseudossapo to obtain the laser-Raman spectrum of the test sample. Compared with an existing echinogorgia pseudossapo analysis and identification technology, the identification method is a pure optical method, needs few sample quantity, has short testing time and has an accurate testing result; the sample is not damaged in a testing process and chemical pollutants are not generated; the established laser-Raman spectrum can be used for rapidly and effectively identifying the echinogorgia pseudossapo sample and provides an effective qualitative identification standard to deep researches.

Description

technical field [0001] The invention belongs to the field of identification of gorgonians, and specifically designs a method for qualitative identification of gorgonians by using laser Raman spectrum. Background technique [0002] Raman spectroscopy is a method that irradiates the target with excitation light, collects its internal molecules and analyzes the scattering spectrum of the excitation light, obtains molecular vibration and rotation information, and applies it to determine the molecular structure and content of the target. . Optical tweezers use highly concentrated laser beams to imprison the target, so as to achieve the effect of fixing it in the solution. Laser tweezers Raman spectroscopy combined with optical tweezers technology is called laser tweezers Raman spectroscopy (LTRS), which makes the target closer to its natural physiological condition, reduces the uncertainty generated when the target moves, and reduces the interference of stray light , which impr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/42
Inventor 高程海何碧娟陈波张荣灿柯珂雷富王一兵
Owner GUANGXI ACAD OF SCI
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