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Bacillus thuringiensis liquid culture medium and preparation method thereof

The technology of Bacillus thuringiensis and liquid culture medium is applied in the field of Bacillus thuringiensis liquid culture medium and its preparation, which can solve the problems of reducing the production cost of biological pesticides, polluting the environment with the distiller's grains, etc., so as to prevent pollution and the loss of useful substances, reduce the Cost, simple effect of extraction process

Inactive Publication Date: 2014-09-17
LUZHOU PINCHUANG TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention not only solves the problem of liquor distiller's grains polluting the environment, but also realizes the full utilization of waste resources in the brewing industry, reduces the production cost of biological pesticides, and turns waste into treasure

Method used

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  • Bacillus thuringiensis liquid culture medium and preparation method thereof
  • Bacillus thuringiensis liquid culture medium and preparation method thereof
  • Bacillus thuringiensis liquid culture medium and preparation method thereof

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preparation example Construction

[0037] The preparation method of the above-mentioned bacillus thuringiensis liquid medium comprises the following steps: adding soybean cake powder, yeast, calcium carbonate, potassium dihydrogen phosphate, manganese sulfate and zinc sulfate to the distiller's grain extract of liquor, mixing and diluting by 3 to 6 times with water , after sterilization is the liquid culture medium of Bacillus thuringiensis.

[0038] Specifically, the centrifugation speed described in the above preparation method is not lower than 10,000 rpm, and the time is not lower than 3 minutes.

[0039] Preferably, the sterilization conditions described in the above preparation method are sterilization at a pressure of 102.9 kPa and a temperature of 121° C. for 20 minutes.

[0040] Use of the above bacillus thuringiensis liquid culture medium for cultivating bacillus thuringiensis strains.

[0041] A Bacillus thuringiensis biopesticide raw powder is obtained by culturing the Bacillus thuringiensis strain i...

Embodiment 1

[0058] The 100kg liquor distiller's grains extract obtained by processing 13 was used, and 2kg of soybean cake powder, ZnSO 4 0.02kg, CaCO 3 0.25kg, MnSO 4 0.02kg, KH 2 PO 4 0.2kg, yeast 0.2kg, mix well, add water to make up to 500L, stir to sterilize. Move the seed liquid to the fermenter according to the volume ratio of the seed liquid to the Bacillus thuringiensis liquid culture medium as 10%, the fermentation conditions are: temperature 30°C, pressure 0.03MPa, stirring speed 230r / min, when the parasporal crystals fall off 20% Stop fermentation. The number of live bacteria is 121×10 8 / ml, the number of spores is 296×10 7 / ml.

Embodiment 2

[0060] Using the 100kg distiller's grains extract obtained from treatment 15, and adding 1kg of soybean cake powder, ZnSO 4 0.01kg, CaCO 3 0.2kg, MnSO 4 0.01kg, KH 2 PO 4 0.1kg, yeast 0.1kg, mix well, add water to 500L, stir to sterilize. Move the seed liquid to the fermenter according to the volume ratio of the seed liquid to the Bacillus thuringiensis liquid culture medium as 10%, the fermentation conditions are: temperature 30°C, pressure 0.03MPa, stirring speed 230r / min, when the parasporal crystals fall off 20% Stop fermentation. The number of live bacteria is 118×10 8 / ml, the number of spores is 168×10 7 / ml.

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Abstract

The invention belongs to the technical field of microbiological culture mediums and in particular relates to a bacillus thuringiensis liquid culture medium and a preparation method thereof. The bacillus thuringiensis liquid culture medium is prepared by the following steps: by taking white spirit distiller grains leaching solution, yellow soybean cake solution, yeasts, calcium carbonate, monopotassium phosphate, manganese sulfate and zinc sulfate as raw materials, uniformly mixing and sterilizing. The bacillus thuringiensis biological pesticide raw powder prepared by using the bacillus thuringiensis liquid culture medium has extremely high insecticidal activity. According to the culture medium, the cost of the bacillus thuringiensis insecticide can be greatly reduced, the problem that the environment is polluted by the white spirit distiller grains is solved, and waste resources in wine-making industry are fully utilized.

Description

technical field [0001] The invention belongs to the technical field of microbial culture medium, and in particular relates to a bacillus thuringiensis liquid culture medium and a preparation method thereof. Background technique [0002] Pests are an important factor that limits the stable and high yield of crops, and the food eaten by pests accounts for about 1 / 4 of the total output every year. For a long time, people have invested a lot of energy to develop chemical pesticides, but they have not been able to solve the problem. At the same time, they are still facing some unsolvable problems, such as human and animal poisoning, environmental pollution and so on. Therefore, with the continuous enhancement of people's awareness of environmental protection, biopesticides have attracted more and more attention. [0003] Bacillus thuringiensis (Bt) is a Gram-positive bacterium with endogenous spores. In the early stage of spore formation, it will form insecticidal crystal protei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07
Inventor 郑琦周军姚祖杰林白容章秋萍马蓉袁瀚沈小娟关雄黄小红
Owner LUZHOU PINCHUANG TECH CO LTD
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