Sesquiterpene compounds and application thereof
A technology for sesquiterpenes and compounds, which is applied in the field of sesquiterpenoids, can solve problems such as no known drugs, and achieve the effects of novel structure, strong antitumor activity and simple preparation method
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[0023] Example 1
[0024] Prepare solid PDA medium (15 L per liter of water is divided into 750 90 mm glass petri dishes, each with about 20 mL of medium, sterilized and connected to activated wild abalone mushroom ( Pleurotus cystidiosus ) ZYB2013 (preserved in the China Type Culture Collection on June 15, 2014, the preservation number is CCTCC M 2014256) mycelial block, cultured in a 28 ℃ constant temperature incubator for 43 days. After cultivation, the wild abalone mushroom mycelium and the culture medium were chopped up, placed in a tap bottle, and an organic solvent (ethyl acetate: methanol: acetic acid volume ratio = 80:15:5) was added for extraction 6 times, and the extract was collected , Concentrated under reduced pressure at 40 ℃ to a paste, the paste was extracted with pure water and ethyl acetate 1:1 6 times, the ethyl acetate phase was dehydrated with anhydrous sodium sulfate, concentrated under reduced pressure at 40 ℃ to a paste, A crude ethyl acetate extract (4....
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[0038] Example 2
[0039] The inhibitory effects of the compounds on prostate cancer cells DU-145, C42B and LNCaP were measured by MTT method. Make the cultured prostate cancer cells DU-145, C42B and LNCaP into a single cell suspension, count and dilute to a cell concentration of 6×10 with a cell plate 4 Pieces / mL. Seed cells in a 96-well plate, 80μL per well. There are also 2 wells with no cells and only 80 μL culture medium [Dulbecco’s modified Eagle’s media (DMEM, Gibco, USA) + 10% calf serum] blank control wells for instrument zero adjustment. Set at 37℃, 5% CO 2 Incubate in the incubator for 24 h, and then add 20 μL of the sample diluted with the culture fluid. At the same time, add 20 μL of cisplatin to the positive control wells, and add 20 μL of culture medium to the negative control wells and blank control wells. Continue to incubate for 72 hours and add 10 μL of 5 mg / mL MTT to each well. React at 37°C for 3 hours, add 100 μL 10% SDS-0.01mol / L HCl to each well to dis...
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