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Process for extracting fisetin from cotinus coggygria

A technology of fisetin and cotinus, applied in organic chemistry and other directions, can solve the problems of complex process and high cost, achieve the effect of short cycle, avoid sample loss, and solve the effect of long separation cycle

Inactive Publication Date: 2014-10-01
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing method of extracting fisetin generally adopts the method of extracting the existing flavonoids. Due to different properties of flavonoids, the extraction and purification methods are also different. The same flavonoids have different extraction methods in different plants. Therefore, the extraction method of flavonoids is too complicated. ,the cost is too high

Method used

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  • Process for extracting fisetin from cotinus coggygria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 5kg of dried branches and leaves of Cotinus cotinus, crush them, pass through a 40-mesh sieve, put them into the equipment cavity, add 8 times the amount of 60% ethanol for extraction, ultrasonic for 20 minutes, the temperature is 30°C, the ultrasonic power is 800w, filter, and the filtrate is concentrated under reduced pressure until it has no alcohol smell. The concentrated solution is passed through an AB-8 macroporous resin column, washed with water until there is no alcohol smell, and then eluted with 6BV50% ethanol, and the alcohol eluent is concentrated and dried to obtain a dry product;

[0022] Mix ethyl acetate-n-butanol-water at a volume ratio of 3:1:4, mix evenly and let stand to separate layers, the lower phase is the mobile phase, the upper phase is the stationary phase, the dried matter is dissolved in the lower phase, and the stationary phase Pump the chromatographic column fully, then adjust the speed of the main engine to 1000rmp, pump in the mobil...

Embodiment 2

[0024] Take 10kg of dried branches and leaves of cotinus sumac, crush them, pass through a 40-mesh sieve, put them into the equipment cavity, add 10 times the amount of 80% ethanol for extraction, ultrasonic for 40 minutes, the temperature is 30°C, the ultrasonic power is 600w, filter, and the filtrate is concentrated under reduced pressure until it has no alcohol smell. The concentrated solution is passed through a XAD-4 macroporous resin column, washed with water until there is no alcohol smell, and then eluted with 8BV60% ethanol, and the alcohol eluent is concentrated and dried to obtain a dry product;

[0025] Mix ethyl acetate-n-butanol-water at a volume ratio of 6:5:7, mix evenly and then let stand to separate. The lower phase is the mobile phase, the upper phase is the stationary phase, and the dried matter is dissolved in the lower phase. Pump the chromatographic column fully, then adjust the speed of the main engine to 900rmp, pump in the mobile phase, the flow rate i...

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Abstract

The invention relates to a process for extracting fisetin from cotinus coggygria. The process comprises the following steps: (1) crushing anacardiaceae plant, namely, cotinus coggygria raw material, and then adding ethanol to preprocess; (2) ultrasonically extracting, filtering, and performing pressure-reduction concentration for the filtrate; (3) absorbing concentration liquid through macroporous resin, washing with water to remove impurity, eluting with ethanol, and collecting the eluant; (4) concentrating and drying the eluant, and separating and purifying through a high-speed counter current chromatograph; and (5) crystallizing. The process has the advantages that the process is simple, the operation is safe and convenient, and the process is suitable for the industrial production.

Description

technical field [0001] The invention belongs to the field of natural medicine extraction, in particular to a process for extracting fisetin from sumac cotinus. Background technique [0002] Fisetin is also known as fisetin, fisetin, fusetin, fisetin, and the molecular formula of fisetin is C 15 h 10 o 6 , the molecular weight is 286.23, and the structural formula is: [0003] [0004] Fisetin is slightly soluble in acetone, methanol, and ethanol, and almost insoluble in ethyl acetate, acetic acid, chloroform, benzene, and acidic aqueous solutions. In alkaline aqueous solutions, the solubility increases with the rise of pH. [0005] The existing method of extracting fisetin generally adopts the method of extracting the existing flavonoids. Due to different properties of flavonoids, the extraction and purification methods are also different. The same flavonoids have different extraction methods in different plants. Therefore, the extraction method of flavonoids is too co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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