Rice blast resisting protein of rice, coding gene and application thereof

Abstract

The invention discloses a rice blast resisting protein of rice, a coding gene and an application thereof. The protein is shown as in (a) or (b), wherein (a) is the protein formed by an amino acid sequence of a sequence 2 in a sequence table; and (b) is the protein which is formed by substituting and / or deleting and / or adding one or several amino acid residues of the amino acid sequence of the sequence 2, is related to the plant rice blast resistance and is derived from (a). PID3-Kn protein and the coding gene thereof can be used for cultivating rice blast resisting new varieties of plants, which can particularly resist the rice blast caused by at least one of the following rice blast bacteria microspecies: 03-10-66-1, 07-31-1-2, 03-10-76-3, 04-8-2-1, 10-25-1-1, 10-32-2-1, 10-128-2-1, 10-62-3-1, 03-11-37-1, 07-55-1-1, 10-120-21-2, 99-20-2, CH43, 99-26-2, Zhong-10-8-14, Chuang ZB15, 97-27-2, Y34, B04, B16 and B23. The rice blast resisting protein is beneficial to improvement of rice varieties, and has significance to increase of crop planting range and crop yield.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and relates to a rice blast resistance protein, its coding gene and its application. Background technique [0002] Plants will be attacked by various pathogenic microorganisms during the growth and development process. Although plants do not have the ability to actively "seek advantages and avoid disadvantages", during the long-term co-evolution process with various pathogenic microorganisms, plants have formed a precise response to pathogenic microorganisms. Signal perception and defense system. Plant innate immunity mainly includes three aspects: basic resistance, resistance mediated by disease resistance genes, and systemic acquired resistance. Among them, resistance mediated by disease resistance genes is an important part of plant disease resistance due to its fast response time and strong disease resistance response, often accompanied by hypersensitivity reactions at the site of pa...

AI Technical Summary

Problems solved by technology

[0005] At present, the cloning of the rice blast resistance gene mainly relies on map-based cloning. Although both the indica rice 9311 and the japonica rice Nipponbare have complete genome sequences, which brings great convenience to the map-based cloning of rice genes, the cloning of the rice blast resistance gene is still difficult. said, there are still many difficulties

For example, the identification of rice blast resistance of each individual plant in a fine-mapped population not only requires a huge workload, but also the incidence of rice blast in the field is easily affected by the environment, resulting in statistical errors in traits and accurate positioning; in addition, from the sequenced According to the 9311 and Nipponbare genome sequences, most of the NBS-LRR genes exist in clusters. Even if the target gene can be located in a certain segment, it is difficult to judge which of the clustered genes is the real disease resistance gene

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