Carotenoid high yield strain and application thereof

A technology of carotene and high-yielding strains, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of high cost and low yield, and achieve the effect of strong radiation resistance and high carotenoid performance

Active Publication Date: 2014-10-01
NANJING UNIV OF TECH
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Problems solved by technology

However, using existing microorganisms to prepare natu

Method used

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  • Carotenoid high yield strain and application thereof
  • Carotenoid high yield strain and application thereof
  • Carotenoid high yield strain and application thereof

Examples

Experimental program
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[0021] Example 1: Strains Deinococcus wulumuqiensis sp. nov. mutagenesis

[0022] Inoculate from frozen bacteria at -80℃ Deinococcus wulumuqiensis sp. nov. (Wang W, Mao J, Zhang Z, Tang Q, Xie Y, Zhu J, Zhang L, Liu Z, Shi Y, Goodfellow M. 2010. Deinococcus wulumuqiensis sp. nov., and Deinococcus xibeiensis sp. nov., isolated from radiation-polluted soil. Int. J. Syst. Evol. Microbiol. 60:2006–2010.) to TGY solid medium, culture at 30℃ for 3-4 days, pick a single colony and inoculate to TGY Liquid culture medium, cultured in a shaker of 150r / min at 30°C for 3-4 days.

[0023] Measure the absorbance (OD value) of the bacterial liquid with a spectrophotometer, and adjust the OD value of the bacterial liquid to 1 with normal saline. Coat the diluted bacterial solution on small iron plates, each small iron plate with 10μl. The small iron flakes coated with bacteria liquid were mutagenized respectively at 0s, 60s, 90s, 120s, 150s, and 180s using an ARTP machine at 100W.

[0024] Af...

Example Embodiment

[0025] Example 2: Identification of strain R12 M

[0026] After microbiological identification, the strain R12 M belongs to the genus Deinococcus.

[0027] The morphological, physiological and biochemical determinations of R12 M strain were carried out with reference to "Berger's Bacteria Identification Manual". The main physiological and biochemical determination results are shown in Table 1. The strain was grown on the surface of TGY solid medium and cultured at 30°C for 14 days. The colony was orange-red, small, round, convex, neat, smooth and opaque; the cells were round and quadruple.

[0028] Table 1 The main physiological and biochemical characteristics of R12 M

[0029]

[0030] Note: "+" in Table 1 is a positive reaction; "-" is a negative reaction; "ND" is no data; "W" is a weak positive reaction.

[0031] The 16S rDNA sequence (SEQ ID NO: 1) of the R12 M strain was obtained by PCR. The sequence determination, BLAST homology comparison and evolutionary analysis showed that R1...

Example Embodiment

[0032] Example 3: γ-ray determination of the radiation resistance of strain R12 M

[0033] Take strain R12 M as the test strain, set Deinococcus wulumuqiensis sp. nov. is the positive control, set Escherichia coli DH 5α It is a negative control. The three strains were grown in TGY liquid medium to the logarithmic growth phase, washed with physiological saline, centrifuged at 4℃, and resuspended in physiological saline to obtain a concentration of 1×10. 7 -1×10 8 cfu / mL bacterial solution. The three bacterial liquids were divided into 2mL aliquots, and exposed to 0.167kGy / min dose in an environment of 25℃. 60 Under the Co radiation source, gamma radiation treatment is performed with a gradient of 2.0 kGy in the range of radiation dose from 0 to 20.0 kGy. Every 10 minutes, take 0.1 mL of irradiated bacteria solution and dilute with normal saline gradually. Take 100 microliters of each dilution gradient to spread on TGY plates and place them in an incubator at 30°C. Count the co...

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Abstract

The invention provides a carotenoid high yield strain and application thereof, and relates to the field of microbe fermentation and metabolite thereof. The carotenoid high yield strain is Deinococcus wulumuqiensis R12M, and the preservation registration number is CCTCC No: M2014099. The invention further provides the application of the strain to the field of preparation of carotenoid. Compared with Deinococcus wulumuqiensis sp.nov. and Deinococcus radiodurans DSM 20539T, the wulumuqiensis R12M, provided by the invention, has better radiation resistance and carotenoid production property, and can be applied to production of natural pigment and natural anti-oxidant.

Description

technical field [0001] The invention relates to the field of microbial fermentation and its metabolites, in particular to carotenoid high-yield bacterial strains and applications thereof. Background technique [0002] Free radicals refer to atoms, molecules or groups containing unpaired electrons. Because free radicals contain unpaired electrons, they have a tendency to pair and have high activity. If free radicals want to be stable, they must snatch electrons from adjacent atoms or molecules to pair their own electrons, but this also makes the atom or molecule from which the electrons are snatched become new free radicals, triggering a chain reaction. This process is "oxidation" . If free radicals are produced too much or cleared too slowly, they will cause various damages to the body at the molecular level, cellular level, and tissue and organ level by attacking life macromolecules and various cells, accelerating the aging process of the body and inducing various disease...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P23/00C12R1/01
Inventor 黄和徐娴金玮玥江凌李霜宋萍
Owner NANJING UNIV OF TECH
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