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Carotenoid high yield strain and application thereof

A technology of carotene and high-yielding strains, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of high cost and low yield, and achieve the effect of strong radiation resistance and high carotenoid performance

Active Publication Date: 2014-10-01
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using existing microorganisms to prepare natural carotenoids, the yield is low and the cost is high

Method used

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  • Carotenoid high yield strain and application thereof
  • Carotenoid high yield strain and application thereof
  • Carotenoid high yield strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment one: bacterial strain Deinococcus wulumuqiensis mutagenesis of sp. nov.

[0022] Inoculate from frozen bacteria stored at -80°C Deinococcus wulumuqiensis sp. nov. (Wang W, Mao J, Zhang Z, Tang Q, Xie Y, Zhu J, Zhang L, Liu Z, Shi Y, Goodfellow M. 2010. Deinococcus wulumuqiensis sp. nov., and Deinococcus xibeiensis sp. nov., isolated from radiation-polluted soil. Int. J. Syst. Evol. Microbiol. 60:2006–2010.) to TGY solid medium, cultured at 30°C for 3-4 days, picked a single colony and inoculated to TGY Liquid medium, cultivated in a 150r / min shaker at 30°C for 3-4 days.

[0023] Measure the absorbance (OD value) of the bacterial solution with a spectrophotometer, and adjust the OD value of the bacterial solution to 1 with physiological saline. Spread the diluted bacterial solution on small iron sheets, 10 μl on each small iron sheet. The small iron sheets coated with the bacterial solution were mutagenized for 0s, 60s, 90s, 120s, 150s, and 180s unde...

Embodiment 2

[0025] Embodiment two: the identification of bacterial strain R12 M

[0026] According to microbiological identification, the strain R12 M belongs to the genus Deinococcus.

[0027] Refer to the "Bergey's Bacteria Identification Manual" and other morphological, physiological and biochemical assays for the R12 M strain. The main physiological and biochemical assay results are shown in Table 1. The strain grows on the surface of TGY solid medium. After 14 days of culture at 30°C, the colony is orange-red, small, round, raised, with neat edges, smooth surface, and opaque; the cells are round and quadruple.

[0028] Table 1 The main physiological and biochemical characteristics of R12 M

[0029]

[0030] Note: In Table 1, "+" means positive reaction; "-" means negative reaction; "ND" means no data; "W" means weak positive reaction.

[0031] The 16S rDNA sequence (SEQ ID NO: 1) of the R12 M strain was obtained by PCR, and sequence determination, BLAST homology comparison and evo...

Embodiment 3

[0032] Embodiment 3: Determination of the radiation resistance of bacterial strain R12 M by gamma rays

[0033] Take the strain R12 M as the test strain, set Deinococcus wulumuqiensis sp. nov. is the positive control, set Escherichia coli DH 5α as a negative control. The three strains were cultured in TGY liquid medium and grown to the logarithmic growth phase, washed with normal saline, centrifuged at 4°C and resuspended with normal saline to obtain a concentration of 1×10 7 -1×10 8 cfu / mL bacterial solution. The three bacterial solutions were divided into 2mL small portions, and exposed to a dose of 0.167kGy / min in an environment of 25°C 60 Under the radioactive source of Co, γ-irradiation treatment was carried out with a gradient of 2.0 kGy in the range of radiation dose from 0 to 20.0 kGy. Every 10 minutes, take 0.1 mL of irradiated bacteria solution, and carry out gradient dilution with physiological saline, and take 100 microliters of each dilution gradient to c...

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Abstract

The invention provides a carotenoid high yield strain and application thereof, and relates to the field of microbe fermentation and metabolite thereof. The carotenoid high yield strain is Deinococcus wulumuqiensis R12M, and the preservation registration number is CCTCC No: M2014099. The invention further provides the application of the strain to the field of preparation of carotenoid. Compared with Deinococcus wulumuqiensis sp.nov. and Deinococcus radiodurans DSM 20539T, the wulumuqiensis R12M, provided by the invention, has better radiation resistance and carotenoid production property, and can be applied to production of natural pigment and natural anti-oxidant.

Description

technical field [0001] The invention relates to the field of microbial fermentation and its metabolites, in particular to carotenoid high-yield bacterial strains and applications thereof. Background technique [0002] Free radicals refer to atoms, molecules or groups containing unpaired electrons. Because free radicals contain unpaired electrons, they have a tendency to pair and have high activity. If free radicals want to be stable, they must snatch electrons from adjacent atoms or molecules to pair their own electrons, but this also makes the atom or molecule from which the electrons are snatched become new free radicals, triggering a chain reaction. This process is "oxidation" . If free radicals are produced too much or cleared too slowly, they will cause various damages to the body at the molecular level, cellular level, and tissue and organ level by attacking life macromolecules and various cells, accelerating the aging process of the body and inducing various disease...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P23/00C12R1/01
Inventor 黄和徐娴金玮玥江凌李霜宋萍
Owner NANJING UNIV OF TECH
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