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Application of gold nanocluster as glutathione fluorescent probe

A technology of gold nanoclusters and fluorescent probes, which is applied in the application field of gold nanoclusters in the detection of glutathione, can solve the problems of sample toxicity and complex glutathione methods, and achieve wide detection range and good distinction Effect, high sensitivity effect

Active Publication Date: 2014-10-15
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the methods for detecting glutathione in the above prior art are complicated, the samples are toxic, and it is impossible to effectively distinguish the interference of cysteine ​​and homocysteine ​​on the detection of glutathione.

Method used

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  • Application of gold nanocluster as glutathione fluorescent probe
  • Application of gold nanocluster as glutathione fluorescent probe
  • Application of gold nanocluster as glutathione fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation method of the gold nanocluster comprises the following steps: 5mL of 10mM chloroauric acid and 15mL of 100mM histidine are reacted in the dark at room temperature for 2h to obtain a gold nanocluster solution with blue-green fluorescence. The TEM results are shown in figure 2 (A), the particle size distribution results are shown in figure 2 (B). Wherein the substance ratio of chloroauric acid and histidine is 1:30.

Embodiment 2

[0030] The preparation method of this gold nanocluster comprises the following steps: the chloroauric acid of 5mL 10mM and the histidine of 10mL 100mM are reacted in the dark at room temperature for 8h, obtain the gold nanocluster solution with blue-green fluorescence, its TEM result and embodiment 1 consistent. Wherein the substance ratio of chloroauric acid and histidine is 1:20.

Embodiment 3

[0032] The preparation method of this gold nanocluster comprises the following steps: the chloroauric acid of 0.25mL 10mM and the histidine of 1.5mL 100mM are reacted in the dark at room temperature for 5h, obtain the gold nanocluster solution with blue-green fluorescence, its TEM result and Embodiment 1 is consistent. Wherein the substance ratio of chloroauric acid and histidine is 1:60.

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Abstract

The invention provides application of a gold nanocluster as a glutathione fluorescent probe. Specifically, the gold nanocluster is prepared by the method of: reacting chloroauric acid with histidine away from light at room temperature for 2-8h. According to the invention, the gold nanocluster is adopted as a fluorescent probe and applied in glutathione detection and cancer cell recognition, and the influence of cysteine, homocysteine and other common biomolecules on glutathione detection can be effectively avoided. The method is simple, the detection range is wide and the sensitivity is high.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the application of gold nanoclusters in the detection of glutathione. Background technique [0002] Glutathione is an important antioxidant and the most abundant (1–15mM) small sulfhydryl molecule in the body. It protects cellular components from damage by reactive oxygen species (ROS), and glutathione is involved in many physiological processes including xenobiotic metabolism, intracellular signal transduction and gene regulation. More importantly, glutathione is involved in the regulation of cancer cell death, including apoptosis, necrosis and autophagy. Elevated levels of glutathione in cancer cells can trigger resistance to chemotherapy drugs such as doxorubicin. Because of the importance of glutathione, a highly sensitive and selective method for the detection of glutathione is needed. [0003] Fluorescence detection is widely used because it will not destroy the sample and h...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 吴富根张晓东陈战
Owner SOUTHEAST UNIV
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