Method for preparing erythrito through fermentation process

A technology of erythritol and fermentation, which is applied in the biological field, can solve the problems of scale and technology that cannot meet the market demand, low strain production performance, and difficulty in large-scale production, so as to avoid oil pollution, high conversion rate, The effect of environmental protection of raw materials

Inactive Publication Date: 2014-10-29

中科医药行业生产力促进中心有限公司

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AI-Extracted Technical Summary

Problems solved by technology

At present, domestic companies such as Shandong Baolingbao and Zibo Zhongshi Gerui use fermentation to produce erythritol, but due to factors such as scale and technology, they cannot meet the market demand.

In recent years, in order to obtain the production of erythritol at home and abroad, the research on the production of erythritol by using hyperosmotic yeast has been carried out...

Abstract

The invention relates to a method for preparing erythrito through a fermentation process. Erythrito is sugar alcohol with the smallest relative molecular mass, found in the nature, has the characteristics of good crystallinity, and cool and refreshing mouthfeel, and is an internationally-popular novel functional sweetener. Erythrito has good food processing adaptability and health care functions, and is widely used in the fields of foods, medicines, cosmetics and the chemical industry now. In the invention, sucrose is adopted as a raw material, high-yielding Moniliella acetoabutans obtained through NTG mutagenesis is used as a fermentation strain, and a fermentation culture medium and a fermentation technology are optimized. The final concentration of erythrito reaches 220g/L, and the fermentation concentration is high, so the method is suitable for industrial production.

Application Domain

Microorganism based processesFermentation

Technology Topic

Chemical industryMutagenic Process +13

Examples

Experimental program(4)

Example Embodiment

[0016] Example 1

[0017] Fermentation strain: Moniliella acetoabutans YZ0806

[0018] Slant medium: 200g glucose, 10g yeast extract, 1g urinary cord, 20g agar, 1000mL distilled water, pH 6.0;

[0019] Seed medium: sucrose 25g, beef extract 5g, yeast extract 7g, peptone 35g, distilled water 1100mL;

[0020] Acetomonas esculentum YZ0806 was inoculated on a slant medium, cultured at 30°C for 72 hours for activation, and activated twice; 3 pieces of 0.5 cm 2 The activated strains were inserted into the sterilized and cooled seed culture medium and cultured at 32°C and 160 rpm for 48 hours; the seed culture obtained was transferred to the sterilized and cooled fermentation medium at 32°C at an inoculum of 10%. The ventilation rate is 0.5vvm, the dissolved oxygen is controlled to 30% during the growth phase of the bacteria, and the dissolved oxygen is adjusted to 15% after 50h, the pH range is 6.0, 180rpm culture for 144h; the fermentation broth of the strain is centrifuged at 4000r/min for 20-30min, up The clear liquid is the crude extract of erythritol, and the erythritol content is 221g/L.

Example Embodiment

[0021] Example 2

[0022] Fermentation strain: Moniliella acetoabutans YZ0806

[0023] Slant medium: 200g glucose, 10g yeast extract, 1g urinary cord, 20g agar, 1000mL distilled water, pH 6.0;

[0024] Seed medium: sucrose 20g, beef extract 3g, yeast extract 5g, peptone 30g, distilled water 1000mL;

[0025] Acetomonas esculentum YZ0806 was inoculated on a slant medium, cultured at 30°C for 72 hours for activation, and activated twice; 3 pieces of 0.5 cm 2 The activated strains were inserted into the sterilized and cooled seed culture medium and cultured at 32°C at 160 rpm for 48 hours; the seed culture obtained was transferred to the sterilized and cooled fermentation medium at 32°C at an inoculum of 10%. The ventilation rate is 0.5vvm, the dissolved oxygen is controlled to 30% during the growth period of the bacteria, and the dissolved oxygen is adjusted to 15% after 50h, the pH range is 5.0, 180rpm culture for 144h; the fermentation broth of the strain is centrifuged at 4000r/min for 20-30min, up The clear liquid is the crude extract of erythritol, and the erythritol content is 225g/L.

Example Embodiment

[0026] Example 3

[0027] Fermentation strain: Moniliella acetoabutans YZ0806

[0028] Slant medium: 200g glucose, 10g yeast extract, 1g urinary cord, 20g agar, 1000mL distilled water, pH 6.0;

[0029] Seed culture medium: 22g sucrose, 4g beef extract, 6g yeast extract, 32g peptone, 1000mL distilled water;

[0030] Acetomonas esculentum YZ0806 was inoculated on a slant medium, cultured at 30°C for 72 hours for activation, and activated twice; 3 pieces of 0.5 cm 2 The activated strains were inserted into the sterilized and cooled seed culture medium and cultured at 32°C at 160 rpm for 48 hours; the seed culture obtained was transferred to the sterilized and cooled fermentation medium at 32°C at an inoculum of 10%. The ventilation rate is 0.5vvm, the dissolved oxygen is controlled at 30% during the growth period of the bacteria, and the dissolved oxygen is adjusted to 15% after 50h, the pH range is 5.5, 180rpm culture for 144h; the fermentation broth of the strain is centrifuged at 4000r/min for 20-30min, up The clear liquid is the crude extract of erythritol, and the erythritol content is 223g/L.

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Classification and recommendation of technical efficacy words

avoid oil pollution
Raw material environmental protection

Method for manufacturing fried food packaging film

ActiveCN104558647AGood oil resistanceavoid oil pollution

Owner:NINGBO HOLLY PRINTING

Method for preparing erythrito through fermentation process

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Examples

Example Embodiment

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

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