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Method for preparing erythrito through fermentation process

A technology of erythritol and fermentation, which is applied in the biological field, can solve the problems of scale and technology that cannot meet the market demand, low strain production performance, and difficulty in large-scale production, so as to avoid oil pollution, high conversion rate, The effect of environmental protection of raw materials

Inactive Publication Date: 2014-10-29
中科医药行业生产力促进中心有限公司
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic companies such as Shandong Baolingbao and Zibo Zhongshi Gerui use fermentation to produce erythritol, but due to factors such as scale and technology, they cannot meet the market demand.
In recent years, in order to obtain the production of erythritol at home and abroad, the research on the production of erythritol by using hyperosmotic yeast has been carried out, but most of them still stay in the isolation and screening of production strains, the optimization of culture conditions and the separation and purification of erythritol At the stage of technical and other laboratory research, the production performance of the strain is low, and the yield is generally between 24.8-54.6mg / mL, making it difficult to carry out large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Fermentation strain: Moniliella acetoabutans YZ0806

[0018] Slant medium: glucose 200g, yeast extract 10g, urethra 1g, agar 20g, distilled water 1000mL, pH6.0;

[0019] Seed medium: 25g sucrose, 5g beef extract, 7g yeast extract, 35g peptone, 1100mL distilled water;

[0020] Bacillus escitoides YZ0806 was inoculated on the slant medium, cultured at 30°C for 72 hours for activation, activated twice; three pieces of 0.5cm 2 The activated strains were inserted into the sterilized and cooled seed medium, and cultured at 32°C, 160rpm for 48h; The ventilation volume is 0.5vvm, the dissolved oxygen is controlled at 30% during the growth period of the bacteria, and the dissolved oxygen is adjusted to 15% after 50 hours, the pH range is 6.0, and the 180rpm is cultivated for 144 hours; the strain fermentation broth is centrifuged at 4000r / min for 20-30min, and the The clear liquid is the crude extract of erythritol, and the content of erythritol is 221g / L.

Embodiment 2

[0022] Fermentation strain: Moniliella acetoabutans YZ0806

[0023] Slant medium: glucose 200g, yeast extract 10g, urethra 1g, agar 20g, distilled water 1000mL, pH6.0;

[0024] Seed medium: 20g sucrose, 3g beef extract, 5g yeast extract, 30g peptone, 1000mL distilled water;

[0025] Bacillus escitoides YZ0806 was inoculated on the slant medium, cultured at 30°C for 72 hours for activation, activated twice; three pieces of 0.5cm 2 The activated strain was inserted into the sterilized and cooled seed medium, and cultured at 32°C, 160rpm for 48h; the seed medium obtained from the cultivation was transferred to the sterilized and cooled fermentation medium at 32°C. The ventilation rate is 0.5vvm, the dissolved oxygen is controlled at 30% during the growth period of the bacteria, and the dissolved oxygen is adjusted to 15% after 50 hours, the pH range is 5.0, and the 180rpm is cultivated for 144 hours; the strain fermentation broth is centrifuged at 4000r / min for 20-30min, and the...

Embodiment 3

[0027] Fermentation strain: Moniliella acetoabutans YZ0806

[0028] Slant medium: glucose 200g, yeast extract 10g, urethra 1g, agar 20g, distilled water 1000mL, pH6.0;

[0029] Seed medium: 22g sucrose, 4g beef extract, 6g yeast extract, 32g peptone, 1000mL distilled water;

[0030] Bacillus escitoides YZ0806 was inoculated on the slant medium, cultured at 30°C for 72 hours for activation, activated twice; three pieces of 0.5cm 2 The activated strain was inserted into the sterilized and cooled seed medium, and cultured at 32°C, 160rpm for 48h; the seed medium obtained from the cultivation was transferred to the sterilized and cooled fermentation medium at 32°C. The ventilation rate is 0.5vvm, the dissolved oxygen is controlled at 30% during the growth period of the bacteria, and the dissolved oxygen is adjusted to 15% after 50h, the pH range is 5.5, and the 180rpm is cultivated for 144h; the strain fermentation broth is centrifuged at 4000r / min for 20-30min, and the The clea...

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Abstract

The invention relates to a method for preparing erythrito through a fermentation process. Erythrito is sugar alcohol with the smallest relative molecular mass, found in the nature, has the characteristics of good crystallinity, and cool and refreshing mouthfeel, and is an internationally-popular novel functional sweetener. Erythrito has good food processing adaptability and health care functions, and is widely used in the fields of foods, medicines, cosmetics and the chemical industry now. In the invention, sucrose is adopted as a raw material, high-yielding Moniliella acetoabutans obtained through NTG mutagenesis is used as a fermentation strain, and a fermentation culture medium and a fermentation technology are optimized. The final concentration of erythrito reaches 220g / L, and the fermentation concentration is high, so the method is suitable for industrial production.

Description

1. Technical field [0001] The invention belongs to the field of biotechnology, in particular to the technology for preparing erythritol by microbial fermentation. 2. Background technology [0002] Erythritol (Erythrito) is the sugar alcohol with the smallest relative molecular mass found so far in nature. It has the characteristics of good crystallinity and cool taste. It is a new type of functional sweetener that is more popular in the world. In addition to having the same health functions as other polysaccharide alcohols, such as preventing dental caries and being suitable for diabetics, erythritol also has two important characteristics, namely low calorie (≤1.66kJ / g) and high tolerance (no side effects ). Because erythritol has good food processing adaptability and health care function, it is widely used in the fields of food, medicine, cosmetics and chemical industry. [0003] Since the discovery of erythritol, more in-depth research has been carried out on its physica...

Claims

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Application Information

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IPC IPC(8): C12P7/18C12R1/645
Inventor 王福清王福华殷红
Owner 中科医药行业生产力促进中心有限公司
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