Method for preparing erythrito through fermentation process
A technology of erythritol and fermentation, which is applied in the biological field, can solve the problems of scale and technology that cannot meet the market demand, low strain production performance, and difficulty in large-scale production, so as to avoid oil pollution, high conversion rate, The effect of environmental protection of raw materials
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Embodiment 1
[0017] Fermentation strain: Moniliella acetoabutans YZ0806
[0018] Slant medium: glucose 200g, yeast extract 10g, urethra 1g, agar 20g, distilled water 1000mL, pH6.0;
[0019] Seed medium: 25g sucrose, 5g beef extract, 7g yeast extract, 35g peptone, 1100mL distilled water;
[0020] Bacillus escitoides YZ0806 was inoculated on the slant medium, cultured at 30°C for 72 hours for activation, activated twice; three pieces of 0.5cm 2 The activated strains were inserted into the sterilized and cooled seed medium, and cultured at 32°C, 160rpm for 48h; The ventilation volume is 0.5vvm, the dissolved oxygen is controlled at 30% during the growth period of the bacteria, and the dissolved oxygen is adjusted to 15% after 50 hours, the pH range is 6.0, and the 180rpm is cultivated for 144 hours; the strain fermentation broth is centrifuged at 4000r / min for 20-30min, and the The clear liquid is the crude extract of erythritol, and the content of erythritol is 221g / L.
Embodiment 2
[0022] Fermentation strain: Moniliella acetoabutans YZ0806
[0023] Slant medium: glucose 200g, yeast extract 10g, urethra 1g, agar 20g, distilled water 1000mL, pH6.0;
[0024] Seed medium: 20g sucrose, 3g beef extract, 5g yeast extract, 30g peptone, 1000mL distilled water;
[0025] Bacillus escitoides YZ0806 was inoculated on the slant medium, cultured at 30°C for 72 hours for activation, activated twice; three pieces of 0.5cm 2 The activated strain was inserted into the sterilized and cooled seed medium, and cultured at 32°C, 160rpm for 48h; the seed medium obtained from the cultivation was transferred to the sterilized and cooled fermentation medium at 32°C. The ventilation rate is 0.5vvm, the dissolved oxygen is controlled at 30% during the growth period of the bacteria, and the dissolved oxygen is adjusted to 15% after 50 hours, the pH range is 5.0, and the 180rpm is cultivated for 144 hours; the strain fermentation broth is centrifuged at 4000r / min for 20-30min, and the...
Embodiment 3
[0027] Fermentation strain: Moniliella acetoabutans YZ0806
[0028] Slant medium: glucose 200g, yeast extract 10g, urethra 1g, agar 20g, distilled water 1000mL, pH6.0;
[0029] Seed medium: 22g sucrose, 4g beef extract, 6g yeast extract, 32g peptone, 1000mL distilled water;
[0030] Bacillus escitoides YZ0806 was inoculated on the slant medium, cultured at 30°C for 72 hours for activation, activated twice; three pieces of 0.5cm 2 The activated strain was inserted into the sterilized and cooled seed medium, and cultured at 32°C, 160rpm for 48h; the seed medium obtained from the cultivation was transferred to the sterilized and cooled fermentation medium at 32°C. The ventilation rate is 0.5vvm, the dissolved oxygen is controlled at 30% during the growth period of the bacteria, and the dissolved oxygen is adjusted to 15% after 50h, the pH range is 5.5, and the 180rpm is cultivated for 144h; the strain fermentation broth is centrifuged at 4000r / min for 20-30min, and the The clea...
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