Preparation for molecularly-imprinted electroluminescent paper chip for detecting multiple sample pesticide residues

A molecular imprinting and luminescence technology, applied in the preparation of paper chips, electroluminescence sensor chips to detect pesticide residues in fruits and vegetables, can solve the problems of many steps in the detection process, unfavorable rapid detection, complex pretreatment, etc. , to achieve fast detection speed, high-throughput screening, and simple operation

Inactive Publication Date: 2014-11-05
UNIV OF JINAN
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Problems solved by technology

Both are commonly used methods for the detection of pesticide residues. Although they have high sensitivity, they have disadvantages such as relatively complicated sample pretreatment, long detection cycle, complicated procedures, and a large number of reagents required.
Chromatography-mass spectrometry method has relatively high sensitivity, but the instrument is expensive and the operation is more complicated;
[0006]3. Although the cost of the enzyme reagent method is relatively low, there are many steps in the detection process and the process is cumbersome, which is not c

Method used

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  • Preparation for molecularly-imprinted electroluminescent paper chip for detecting multiple sample pesticide residues
  • Preparation for molecularly-imprinted electroluminescent paper chip for detecting multiple sample pesticide residues

Examples

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Embodiment example 1

[0046] Implementation Case 1 (Dimethoate detection)

[0047] A method for preparing a molecularly imprinted electroluminescent paper chip for detecting pesticide residues in multiple samples, comprising the following steps:

[0048] (1) Prepare folded paper chips: first print on A4 paper with a wax printer as attached figure 1 Put the wax pattern on the A4 paper with the wax pattern in a flat heater or an oven, and heat it at 60-150 ℃ for 0.5-2 min, so that the wax melts and soaks through the entire thickness of the paper to form a hydrophobic area; The method of screen-printing the electrode, printing the carbon working electrode on the non-printed hydrophilic area of ​​the treated A4 paper (attached figure 2 a), counter electrode (Fig. 2 b) and Ag / AgCl reference electrode (Fig. figure 2 c);

[0049] (2) Select the functional monomer methacrylic acid (MAA) that can synthesize MIPs with dimethoate;

[0050] (3) Dimethoate, the template molecule of residual pesticides,...

Embodiment example 2

[0059] Implementation Case 2 (Detection of parathion)

[0060] A method for preparing a molecularly imprinted electroluminescent paper chip for detecting pesticide residues in multiple samples, comprising the following steps:

[0061] (1) Prepare folded paper chips: first print on A4 paper with a wax printer as attached figure 1 Put the wax pattern on the A4 paper with the wax pattern in a flat heater or an oven, and heat it at 60-150 ℃ for 0.5-2 min, so that the wax melts and soaks through the entire thickness of the paper to form a hydrophobic area; The method of screen-printing the electrode, printing the carbon working electrode on the non-printed hydrophilic area of ​​the treated A4 paper (attached figure 2 a), counter electrode (Fig. 2 b) and Ag / AgCl reference electrode (Fig. figure 2 c);

[0062] (2) Select the functional monomer o-nitrothiophenol that can synthesize MIPs with parathion;

[0063] (3) The template molecule parathion, the functional monomer o-nit...

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Abstract

The invention discloses a preparation for a molecularly-imprinted electroluminescent paper chip for detecting multiple sample pesticide residues. The preparation method of the sensing paper chip comprises the following steps: preparing an MIPs sol of a pesticide residue molecule; preparing a tridimensional graphene gold nanometer composite material; preparing a g-C3N4 quantum dot; and utilizing an electrode modification technology to modify the electrode surface of the sensing paper chip with the tridimensional graphene gold nanometer composite material, the g-C3N4 quantum dot and the MIPs sol. A method for detecting multiple sample pesticide residues comprises the following steps: connecting the modified sensing paper chip to an electrochemical workstation, and cooperating with a chemiluminescence apparatus to detect pesticide residues in a sample extraction solution. The prepared paper chip is strong in electrode specificity and high in sensitivity, and has the sensitivity up to nanometer gram grade. A detection process only takes 3-5 min, and the cost is low. The electrode detection method for pesticide residues is simple and rapid in operation, and reactions and results are automatically finished and recorded by an instrument.

Description

technical field [0001] The present invention relates to the technical field of pesticide residue detection, and more specifically to the preparation of a paper chip for detecting pesticide residues in multiple samples by molecularly imprinted electroluminescence. The present invention also relates to the use of the electroluminescent sensor chip to detect fruits, Methods of Pesticide Residues in Vegetables. Background technique [0002] In recent years, with the improvement of the quality of life, food safety issues have received more and more attention. However, with the development of agricultural technology, more and more pesticides are used in the production of fruits and vegetables. The potential harm of pesticide residues in food to the environment and human health has gradually attracted people's attention. Case reports of pesticide residues in food causing acute or chronic poisoning to humans are also common, which has aroused widespread concern. [0003] Since peo...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N27/26G01N27/30
Inventor 于京华吴鲁丹颜梅葛慎光张彦吴开庆孙国强李丽
Owner UNIV OF JINAN
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