Preparation for molecularly-imprinted electroluminescent paper chip for detecting multiple sample pesticide residues

Abstract

The invention discloses a preparation for a molecularly-imprinted electroluminescent paper chip for detecting multiple sample pesticide residues. The preparation method of the sensing paper chip comprises the following steps: preparing an MIPs sol of a pesticide residue molecule; preparing a tridimensional graphene gold nanometer composite material; preparing a g-C3N4 quantum dot; and utilizing an electrode modification technology to modify the electrode surface of the sensing paper chip with the tridimensional graphene gold nanometer composite material, the g-C3N4 quantum dot and the MIPs sol. A method for detecting multiple sample pesticide residues comprises the following steps: connecting the modified sensing paper chip to an electrochemical workstation, and cooperating with a chemiluminescence apparatus to detect pesticide residues in a sample extraction solution. The prepared paper chip is strong in electrode specificity and high in sensitivity, and has the sensitivity up to nanometer gram grade. A detection process only takes 3-5 min, and the cost is low. The electrode detection method for pesticide residues is simple and rapid in operation, and reactions and results are automatically finished and recorded by an instrument.

Description

technical field [0001] The present invention relates to the technical field of pesticide residue detection, and more specifically to the preparation of a paper chip for detecting pesticide residues in multiple samples by molecularly imprinted electroluminescence. The present invention also relates to the use of the electroluminescent sensor chip to detect fruits, Methods of Pesticide Residues in Vegetables. Background technique [0002] In recent years, with the improvement of the quality of life, food safety issues have received more and more attention. However, with the development of agricultural technology, more and more pesticides are used in the production of fruits and vegetables. The potential harm of pesticide residues in food to the environment and human health has gradually attracted people's attention. Case reports of pesticide residues in food causing acute or chronic poisoning to humans are also common, which has aroused widespread concern. [0003] Since peo...

AI Technical Summary

Problems solved by technology

Both are commonly used methods for the detection of pesticide residues. Although they have high sensitivity, they have disadvantages such as relatively complicated sample pretreatment, long detection cycle, complicated procedures, and a large number of reagents required.

Chromatography-mass spectrometry method has relatively high sensitivity, but the instrument is expensive and the operation is more complicated;

[0006]3. Although the cost of the enzyme reagent method is relatively low, there are many steps in the detection process and the process is cumbersome, which is not conducive to rapid detection;

[0007]4. Although the immunoassay has strong specificity and high sensitivity, it also has disadvantages such as relatively complicated sample pretreatment, long detection cycle, complicated procedures, and various reagents required;

[0008]5. The microbiological method has the advantages of low cost and time saving, but it is generally only used for screening experiments;

[0009] There are some problems and shortcomings in the above methods for the detection and analysis of pesticide residues, which limit their application in actual sample detection

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