A high-yield oil lotus garden green coccus and its application

A technology of green algae and biodiesel, which is applied in the field of high-yield oil-producing green algae, can solve the problems of slow growth of algae species, high equipment cost, and small cell volume, so as to reduce the demand for nutrients and reduce the The effect of culture cost and large cell volume

Active Publication Date: 2016-10-26
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1) The growth rate of algae species is slow and the biomass is low, especially in the period of rapid oil accumulation, the biomass of most algae species is less than 4.0g L -1 , unable to meet the demand for raw materials for downstream biodiesel refining
[0004] 2) Many microalgae can only accumulate a relatively high content of oil under adverse conditions, but the growth of microalgae is severely inhibited under adverse conditions, resulting in a decrease in biomass accumulation, while in the normal culture stage, its oil content is less than 30% , unable to meet the needs of large-scale production
[0006] 4) Some oil-producing microalgae, such as Chlorella, have small cell volume and are difficult to harvest. Currently, the only harvesting method for this type of microalgae is high-speed centrifugation, which has high equipment costs and high energy consumption, resulting in microalgae biogenesis. Diesel costs increase
[0007] 5) Insufficient research and development of coupled culture methods for oleaginous microalgae
[0019] Through the analysis of the oil-producing algae strains that have applied for patent protection, it is found that although some oil-producing microalgae have high oil content, their biomass concentration is low, and their industrialization potential is not obvious; there are also some oil-producing microalgae that exist Problems such as high harvesting cost and weak anti-biological pollution ability

Method used

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  • A high-yield oil lotus garden green coccus and its application
  • A high-yield oil lotus garden green coccus and its application
  • A high-yield oil lotus garden green coccus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The Chlorococcum lianyuanensis of the present invention is isolated from an artificial lake with shallow water depth. The Chlorococcum lianyuanensis is single-celled, oval, with thinner or irregularly thickened cell walls, and pigment bodies in newborn cells. Periphyte, cup-shaped, with 1 pyrenoid, the cells gradually mature and then disperse and fill the whole cell; 1 nucleus, many starch granules; asexual reproduction forms many zoospores, occasionally also forms iminetospores ( figure 1 ). The cell size of Chlorococcus lienyuanensis is 8-12μm, and the cells are relatively large.

[0041] 1. Environmental conditions:

[0042] The suitable growth temperature for Chlorococcum lianyuanensis is 15-30℃, and the light intensity is 10-1000μmol photons m -2 the s -1 , pH 6-10, available in CO 2 The concentration is 0-10% under the condition of aeration.

[0043] 2. Nutritional conditions:

[0044] a).NaNO 3 100-1500mg / L; b).K 2 HPO 4 ·3H 2 O10-100mg / L; c).MgSO 4 ·7...

Embodiment 2

[0052] Example 2: Lianyuan Chlorococcus treatment of domestic wastewater

[0053] 1. Wastewater source

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Abstract

The invention discloses a high-yielding chlorococcus oleifera and its application. Chlorococcum lianyuanensis of the present invention was preserved on December 6, 2013 in the General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee, address: No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, China Institute of Microbiology, Academy of Sciences, deposit number: CGMCC No.8605. Chlorococcus lotusensis can be used in the production of biodiesel, treatment of domestic wastewater, production of pigments, fixation of carbon dioxide or as open bait in fish farming. CGMCC No. 8605 20131206

Description

Technical field: [0001] The invention belongs to the technical field of microbes and bioenergy, and in particular relates to a high-yield chlorococcus algae and its application. Background technique: [0002] Microalgae is an ideal raw material for biodiesel production, but the high production cost restricts its industrialization process. The production of biodiesel from microalgae needs to obtain oil-producing microalgae strains with excellent properties, but the reported oil-producing microalgae There are still many shortcomings: [0003] 1) The growth rate of algae species is slow and the biomass is low, especially in the period of rapid oil accumulation, the biomass of most algae species is less than 4.0g L -1 , unable to meet the demand for raw materials for downstream biodiesel refining. [0004] 2) Many microalgae can only accumulate a relatively high content of oil under adverse conditions, but the growth of microalgae is severely inhibited under adverse conditions...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C02F3/32C12R1/89
Inventor 袁振宏许瑾王忠铭孔晓英朱顺妮
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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