A high-yield oil lotus garden green coccus and its application

Abstract

The invention discloses a high-yielding chlorococcus oleifera and its application. Chlorococcum lianyuanensis of the present invention was preserved on December 6, 2013 in the General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee, address: No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, China Institute of Microbiology, Academy of Sciences, deposit number: CGMCC No.8605. Chlorococcus lotusensis can be used in the production of biodiesel, treatment of domestic wastewater, production of pigments, fixation of carbon dioxide or as open bait in fish farming. CGMCC No. 8605 20131206

Description

Technical field:

[0001] The invention belongs to the technical field of microbes and bioenergy, and in particular relates to a high-yield chlorococcus algae and its application. Background technique:

[0002] Microalgae is an ideal raw material for biodiesel production, but the high production cost restricts its industrialization process. The production of biodiesel from microalgae needs to obtain oil-producing microalgae strains with excellent properties, but the reported oil-producing microalgae There are still many shortcomings:

[0003] 1) The growth rate of algae species is slow and the biomass is low, especially in the period of rapid oil accumulation, the biomass of most algae species is less than 4.0g L -1 , unable to meet the demand for raw materials for downstream biodiesel refining.

[0004] 2) Many microalgae can only accumulate a relatively high content of oil under adverse conditions, but the growth of microalgae is severely inhibited under adverse conditions...

Examples

Embodiment 1

[0040] The Chlorococcum lianyuanensis of the present invention is isolated from an artificial lake with shallow water depth. The Chlorococcum lianyuanensis is single-celled, oval, with thinner or irregularly thickened cell walls, and pigment bodies in newborn cells. Periphyte, cup-shaped, with 1 pyrenoid, the cells gradually mature and then disperse and fill the whole cell; 1 nucleus, many starch granules; asexual reproduction forms many zoospores, occasionally also forms iminetospores ( figure 1 ). The cell size of Chlorococcus lienyuanensis is 8-12μm, and the cells are relatively large.

[0041] 1. Environmental conditions:

[0042] The suitable growth temperature for Chlorococcum lianyuanensis is 15-30℃, and the light intensity is 10-1000μmol photons m -2 the s -1 , pH 6-10, available in CO 2 The concentration is 0-10% under the condition of aeration.

[0043] 2. Nutritional conditions:

[0044] a).NaNO 3 100-1500mg / L; b).K 2 HPO 4 ·3H 2 O10-100mg / L; c).MgSO 4 ·7...

Embodiment 2

[0052] Example 2: Lianyuan Chlorococcus treatment of domestic wastewater

[0053] 1. Wastewater source

Technical field:

[0001] The invention belongs to the technical field of microbes and bioenergy, and in particular relates to a high-yield chlorococcus algae and its application. Background technique:

[0002] Microalgae is an ideal raw material for biodiesel production, but the high production cost restricts its industrialization process. The production of biodiesel from microalgae needs to obtain oil-producing microalgae strains with excellent properties, but the reported oil-producing microalgae There are still many shortcomings:

[0003] 1) The growth rate of algae species is slow and the biomass is low, especially in the period of rapid oil accumulation, the biomass of most algae species is less than 4.0g L -1 , unable to meet the demand for raw materials for downstream biodiesel refining.

[0004] 2) Many microalgae can only accumulate a relatively high content of oil under adverse conditions, but the growth of microalgae is severely inhibited under adverse conditions...

Examples

Embodiment 1

[0040] The Chlorococcum lianyuanensis of the present invention is isolated from an artificial lake with shallow water depth. The Chlorococcum lianyuanensis is single-celled, oval, with thinner or irregularly thickened cell walls, and pigment bodies in newborn cells. Periphyte, cup-shaped, with 1 pyrenoid, the cells gradually mature and then disperse and fill the whole cell; 1 nucleus, many starch granules; asexual reproduction forms many zoospores, occasionally also forms iminetospores ( figure 1 ). The cell size of Chlorococcus lienyuanensis is 8-12μm, and the cells are relatively large.

[0041] 1. Environmental conditions:

[0042] The suitable growth temperature for Chlorococcum lianyuanensis is 15-30℃, and the light intensity is 10-1000μmol photons m -2 the s -1 , pH 6-10, available in CO 2 The concentration is 0-10% under the condition of aeration.

[0043] 2. Nutritional conditions:

[0044] a).NaNO 3 100-1500mg / L; b).K 2 HPO 4 ·3H 2 O10-100mg / L; c).MgSO 4 ·7...

Embodiment 2

[0052] Example 2: Lianyuan Chlorococcus treatment of domestic wastewater

[0053] 1. Wastewater source

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