Application of rice osmads27 gene in promoting lateral root growth of taproot plants

Abstract

The invention discloses application of paddy rice genes OsMADS27 to promoting tap root system plant lateral root growth. The base sequence of the genes OsMADS27 is shown in SEQ ID No.1. The application includes the steps that the paddy rice genes OsMADS27 are connected into a plant expression vector, and a recombinant expression vector is obtained through establishment; the recombinant expression vector is transformed into a receiver plant. According to the application, by cloning the paddy rice genes OsMADS27 and conducting genetic transformation on tap root system plants such as arabidopsis thaliana through the genes, external source paddy rice genes OsMADS27 are over-expressed through the plants, and therefore generation and growth of plant lateral roots can be promoted. The test result shows that an external source NO3- and the genes OsMADS27 are cooperative in promoting growth of the receiver plant. By the adoption of the application, nitrogen key controlling genes relevant to paddy rice lateral root growth can be researched, and the application has significance in screening and breeding plant varieties capable of efficiently absorbing nutrients.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to the application of rice OsMADS27 gene in promoting the growth of lateral roots of taproot plants. Background technique [0002] Rice is one of the most important food crops in the world, and my country is the largest producer and consumer of rice in the world. The successful use of heterosis has greatly increased the yield of rice and made a great contribution to solving the food crisis in the world. But hybrid rice production has stagnated since the 1980s. The input of nitrogen fertilizer in rice production is becoming one of the powerful measures to increase yield, but the input of a large amount of nitrogen fertilizer not only reduces the nitrogen use efficiency, but also brings a series of environmental problems such as waste of resources and eutrophication of water bodies. At present, people adjust in two aspects to improve nitrogen use efficiency, one i...

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Problems solved by technology

[0004] Although the above research results have accumulated very valuable raw data for the study of nitrogen on the morphogenesis of rice roots and the uptake and utilization of nitrogen by roots, most of these studies focus on the observation of phenomena and the determination of changes in substance content. However, there is no profound explanation of the internal cause of the change, so there is still a long way to go to understand the nature of the relationship between nitrogen and rice root growth.

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