Process of preparing D-galactose by extracting cottonseed meal
An extraction process, cottonseed meal technology, applied in the field of extraction and preparation of D-galactose from cottonseed meal, can solve the problems of high cost, many components, and only focus on the purification method of raffinose, etc.
Inactive Publication Date: 2014-11-12
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology
The disadvantage of using the percolation method to extract raffinose from cottonseed meal is that it needs to continuously add fresh solvent to the extract at room temperature, which consumes a large amount of solvent, and the subsequent concentration takes too long, resulting in excessive cost
One is extraction with water as a solvent (CN201110270426.5), because the polarity of the solvent is too large, so that the extracted components are more, and the later purification is more complicated
One is to use alcohol to directly extract raffinose from cottonseed meal (CN201210353993.1; CN200710014172.4; CN200810089636.2). The disadvantage of this method is that it only pays attention to the purification method of raffinose and fails to guarantee the extraction of raffinose from cottonseed meal. Maximally extract more raffinose, what the present invention pays attention to is to be able to obtain the maximum raffinose production value in the minimum energy consumption, obtain the maximum economic benefit
However, there have never been related patent reports on the preparation of D-galactose from raffinose and the preparation of D-galactose from cottonseed meal
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[0009] specific implementation plan
[0010] The present invention utilizes the condensation reflux extraction process to extract the cottonseed meal. The present invention adopts the following scheme: Weigh 15g of cottonseed meal and place it in a round-bottomed flask, add the extractant, condense and reflux extraction under a water bath, collect and filter after cooling, and use ultraviolet Absorbance values were measured with a spectrophotometer.
[0011] The ethanol concentration is 0%-90%, the solid-liquid ratio is 1:6-1:14, the extraction temperature is 50°C-100°C, and the extraction time is 0.5h-3.5h.
[0012] Table 1 The experimental factor level table of extracting galactose from cottonseed cypress
[0013]
[0014] Table 2 Uniform design table and results
[0015]
[0016] As a preferred solution, the process parameters of the present invention are: the ethanol concentration is 70%, the solid-liquid ratio is 1:7, the extraction temperature is 75° C., and th...
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The invention relates to a process of preparing D-galactose by extracting cottonseed meal. Based on present extraction methods, the process allows the content of raffinose obtained from a cottonseed meal extraction liquid to be the highest and allows the content of protein to be the lowest. The process parameters of the process are that: the ethanol concentration is 0-90%, the material liquid ratio is 1:6-1:14, the extraction temperature is 50-100 DEG C and the extraction time is 0.5-3.5 h. The raffinose is hydrolyzed by utilization of an immobilized alpha-galactosidase specific hydrolysis method to obtain the D-galactose. Results are subjected to quantitative analysis by utilization of a gas chromatography analysis method to obtain optimum hydrolysis conditions. The yield of the D-galactose is 8.0-10.0 mg / g. A side product that is saccharose is removed by a method of microbial fermentation by utilization of native strains. The process is feasible in the industry and can allow the result of the process of preparing the galactose from the cottonseed meal to be the maximum.
Description
1. Technical field [0001] The invention relates to a process for extracting and preparing D-galactose from cottonseed meal. 2. Background technology [0002] Cottonseed meal is a by-product obtained from cottonseed after delinting, shelling, kernel shell separation, and oil extraction through pre-press extraction or direct solvent extraction. At present, the development and utilization of cottonseed meal is limited to the production of feed in China, such as the production of feed protein, low-phenol cotton protein, and the production of functional sugar-raffinose. [0003] D-galactose (CH2OH(CHOH)4CHO) is a monosaccharide composed of six carbons and an aldehyde group, classified as aldose and hexose, and is a component of lactose in mammalian milk. D-galactose In the plant kingdom, it often exists in a variety of plant gums in the form of polysaccharides. For example, K-carrageenan in red algae is a polysaccharide composed of D-galactose and 3,6-endether-D-galactose. Free...
Claims
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Login to View More IPC IPC(8): C12P19/14C12P19/02
Inventor 于瑞莲浦军平许金国杨云菲
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE