Method for simultaneously measuring content of nitrogen and phosphorus of plant
A measurement method and plant technology, applied in the direction of analyzing materials through chemical reactions, and analyzing materials through observing the influence of chemical indicators, can solve the problems of poor stability of nitrogen test reagents, high absorption value of phosphorus test reagents, and testing issues such as baseline elevation
Inactive Publication Date: 2014-11-12
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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[0003] The purpose of the present invention is to provide a method for simultaneous determination of plant nitrogen and phosphorus to solve the problems of poor stability of nitrogen test reagents, high absorption value of phosphorus test reagents and continuous rise of test baselines in the prior art
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[0049] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. All the reagents used in the present invention are of analytical grade unless otherwise specified, and the water is deionized water.
[0050] When a kind of plant nitrogen, phosphorus measuring method of the present invention is implemented, earlier prepare sample liquid to be tested, nitrogen and phosphorus test reagent, nitrogen and phosphorus mixed standard solution, then measure with continuous flow analyzer (Germany BRAN-LUEBBE, AA3), Finally, the nitrogen and phosphorus contents of the plant samples were calculated according to the weighing amount of the samples and the dilution factor. Proceed as follows:
[0051] 1) Preparation of sample liquid to be tested: Weigh uniform dry plant samples passed through a 1mm sieve, each sample is about 0.06g ~ 0.1g (accurate to 0.0001g), put it in a 25ml graduated digestion tube, add 1.5ml sul...
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The invention relates to a method for simultaneously measuring the content of nitrogen and phosphorus of a plant based on a continuous flow analyzer. The method comprises the steps of decomposing a plant sample by sulfuric acid-hydrogen peroxide, preparing sample liquid to be measured, preparing a nitrogen and phosphorus mixed standard solution, preparing a nitrogen test reagent and a phosphorus test reagent, setting the reagent flow path and flow rate in a nitrogen test channel and the reagent flow path and flow rate in a phosphorus test channel, feeding the sample liquid to be measured for once by an automatic sampler, and simultaneously measuring the content of nitrogen and phosphorus by the continuous flow analyzer. According to the method, the service life of a nitrogen test buffer solution and the service life of alkaline developing liquid in the nitrogen test reagent are prolonged, the dosage of sodium hydroxide in the nitrogen test buffer solution can be reduced, and the dosage of DCI in the alkaline developing liquid can be also reduced; a phosphorus test base line is stable, and the correcting value of a phosphorus test result is small, so that the reliability of the sample phosphorus test result is improved.
Description
technical field [0001] The invention belongs to the technical field of plant nutrition, and in particular relates to a method for simultaneously measuring plant nitrogen and phosphorus. Background technique [0002] At present, the principle of simultaneous determination of plant nitrogen (N) and phosphorus (P) content by continuous flow analysis method (abbreviated as CFA method) is basically the same, that is, the organic nitrogen and organic phosphorus in the sample are digested by sulfuric acid and hydrogen peroxide, oxidized and decomposed, Convert them into inorganic ammonium salt and inorganic phosphate respectively, and use the CFA method to simultaneously measure the nitrogen and phosphorus content in the digestion liquid; the nitrogen is determined by using disodium hydrogen phosphate solution as the buffer solution, potassium sodium tartrate as the complexing agent, and nitroso Sodium ferricyanide is used as a catalyst. Under alkaline conditions, the ammonium in t...
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Inventor 罗雪华吴小平王文斌郭海超邹碧霞贝美容
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI