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Application of chelerythrine in the preparation of antifungal biofilm drugs

A technology of chelerythrine and biofilm, which is applied in the field of medicine, can solve the problems that affect the prognosis of patients, cannot obtain the expected effect, and the anti-fungal drugs do not have anti-biofilm activity, etc., and achieve the effect of inhibiting growth and proliferation

Inactive Publication Date: 2016-09-07
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in vivo catheter or biological device-associated fungal infections that grow in the form of biofilms and exhibit high drug resistance pose great difficulties to clinical treatment, forcing clinicians to remove catheters or biodevices from patients while performing antifungal therapy. biodevices, thereby affecting patient outcomes
[0006] However, in the prior art, drugs with antifungal activity often do not have antibiofilm activity, resulting in that the expected effect cannot be obtained when it is used to treat fungi that grow in the form of biofilm

Method used

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  • Application of chelerythrine in the preparation of antifungal biofilm drugs
  • Application of chelerythrine in the preparation of antifungal biofilm drugs
  • Application of chelerythrine in the preparation of antifungal biofilm drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1: The effect of chelerythrine alone on the biofilm of different Candida albicans strains

[0080] Materials and Method

[0081] 1. Test drug

[0082] Celandine: purchased from the National Institute for the Control of Pharmaceutical and Biological Products.

[0083] Fluconazole: purchased from Sigma.

[0084] Amphotericin B: purchased from Sigma.

[0085] XTT: purchased from Sigma Company.

[0086] Menadione: purchased from Sigma.

[0087] Dimethyl sulfoxide (DMSO): purchased from Sigma.

[0088] Acetone: purchased from Sigma Company.

[0089] Chelidonine was prepared with dimethyl sulfoxide into a 64mmol / L solution, amphotericin B was prepared with dimethyl sulfoxide into a 9.6mmol / L solution, and fluconazole was made into a 320mmol / L solution with dimethyl sulfoxide. Store the test drug at -20°C. Before the experiment, the drug storage solution was taken out and placed in a 35°C incubator to melt, mix well, and conduct pharmacodynamic experiments respectively.

[0090] XTT...

Embodiment 2

[0122] Example 2: The effect of chelerythrine alone on the biofilm of different Cryptococcus neoformans strains

[0123] Materials and Method

[0124] 1. Test drug

[0125] Celandine: purchased from the National Institute for the Control of Pharmaceutical and Biological Products.

[0126] Fluconazole: purchased from Sigma.

[0127] Amphotericin B: purchased from Sigma.

[0128] XTT: purchased from Sigma Company.

[0129] Menadione: purchased from Sigma.

[0130] Dimethyl sulfoxide (DMSO): purchased from Sigma.

[0131] Acetone: purchased from Sigma Company.

[0132] Chelidonine was prepared with dimethyl sulfoxide into a 64mmol / L solution, amphotericin B was prepared with dimethyl sulfoxide into a 9.6mmol / L solution, and fluconazole was made into a 320mmol / L solution with dimethyl sulfoxide. Store the test drug at -20°C. Before the experiment, the drug storage solution was taken out and placed in a 35°C incubator to melt, mix well, and conduct pharmacodynamic experiments respectively.

[01...

Embodiment 3

[0163] Example 3: The effect of chelerythrine alone on the biofilm of different Aspergillus strains

[0164] Materials and Method

[0165] 1. Test drug

[0166] Celandine: purchased from the National Institute for the Control of Pharmaceutical and Biological Products.

[0167] Fluconazole: purchased from Sigma.

[0168] Amphotericin B: purchased from Sigma.

[0169] XTT: purchased from Sigma Company.

[0170] Menadione: purchased from Sigma.

[0171] Dimethyl sulfoxide (DMSO): purchased from Sigma.

[0172] Acetone: purchased from Sigma Company.

[0173] Chelidonium was prepared with dimethyl sulfoxide to make a 32mmol / L solution, amphotericin B was made with dimethyl sulfoxide to make a 9.6mmol / L solution, and fluconazole was made with dimethyl sulfoxide to make a 320mmol / L solution. Store the test drug at -20°C. Before the experiment, the drug storage solution was taken out and placed in a 35°C incubator to melt, mix well, and conduct pharmacodynamic experiments respectively.

[0174] XTT...

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Abstract

The invention relates to the technical field of medicine. Chelidonium is an isoquinoline alkaloid present in plants such as celandine and bollia, and its structural formula is shown in (I). The invention provides the application of chelerythrine in the preparation of antifungal biofilm drugs, medical equipment or medical materials and the like. Experiments of the present invention prove that chelerythrine has good antifungal biofilm activity both in vitro and in vivo, can effectively inhibit the growth and proliferation of fungal biofilm, and can effectively inhibit mature biofilm.

Description

Technical field [0001] The invention relates to the technical field of medicine, in particular to the application of chelerythrine in the preparation of antifungal biofilm drugs. Background technique [0002] Cheerythrine (chelerythrine) is an isoquinoline alkaloid that exists in plants such as chelerythrine and chelerythrine. Its structural formula is as shown in (I) and molecular formula C 21 H 18 NO 4 + , Molecular weight: 348.37, melting point 195-205°C. [0003] [0004] Chelidonine has rich medicinal value, can inhibit a variety of pathogenic bacteria, and has anti-tumor and insecticidal activities. The Natural Medicine Research Center of Kunming General Hospital, Meng F, et al. reported on the antifungal activity of chelerythrine against human pathogenic fungi (Meng F, Zuo G, Hao X, Wang G, Xiao H, Zhang J, Xu G. Antifungal activity of the benzo[c]phenanthridine alkaloids from Chelidoniummajus Linn against resistant clinical yeast isolates.J Ethnopharmacol.2009;125(3):494-6...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01P3/00A61K31/4741A61P31/10A61L31/16A61L15/44A61L29/16
Inventor 王彦胡淦海胡丹丹毕爽阎澜金磊姜远英
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY