A strain of Arthrobacter tobacco and its application in aquaculture

An Arthrobacter nicotianae, aquaculture technology, applied in bacteria, biological water/sewage treatment, microorganism-based methods, etc., can solve problems such as economic loss, increased eutrophication, frequent fish and shrimp diseases, etc. Strong degradation ability, remarkable effect of water purification

Active Publication Date: 2016-07-06
青岛蔚蓝龙初生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, my country's aquaculture industry has developed rapidly, and the scale of intensive and high-density farming has been expanding day by day. Concentrated bait feeding has resulted in the accumulation of a large amount of residual bait, feces, and dead animals and plants in the aquaculture water, resulting in a serious excess of nitrogen content in the water body and eutrophication. The aggravation of globalization has caused the destruction of the aquaculture ecological environment, frequent occurrence of fish and shrimp diseases, and ultimately caused huge economic losses

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Isolation, screening and identification of embodiment 1 bacterial strain

[0012] 1. Sample

[0013] Water samples taken from the wastewater treatment tower of salmon recirculating aquaculture in Yantai Oriental Ocean Group;

[0014] 2. Formula of heterotrophic nitrosation medium:

[0015] Substance name

Dosage

(NH 4 ) 2 SO 4

0.5g

sodium succinate

5.62g

Vickers salt solution

50.0mL

h 2 o

Dilute to 1000.0mL

[0016] 3. Screening method

[0017] First, gradiently dilute the above water sample: take 0.1mL of the diluted water sample and evenly spread it on the surface of the above heterotrophic nitrosation medium, put it in a constant temperature incubator, and culture it at 30°C for 2-3 days, then pick out the medium with an inoculation loop The single colonies above were separated and purified, which were the primary screening strains.

[0018] A total of 10 single colonies were picked in this exper...

Embodiment 2

[0029] Example 2 Arthrobacter tobacco YY-9 treatment of aquaculture water experiments

[0030] 1. Preparation of bacteria powder

[0031] Firstly, Arthrobacter tobacco YY-9 is activated, expanded and cultivated, then fermented in a liquid state and spray-dried to make bacterial powder. The content of Arthrobacter tobacco is about 10 billion / g.

[0032] 2. Experimental location: small shed shrimp breeding pond in Rudong, Jiangsu

[0033]Three aquaculture ponds with similar water quality indicators were selected as three parallels, and the water bodies in the aquaculture ponds were separated by plastic sheets, half of the water bodies were used as the experimental group, and the other half of the water bodies were used as the control group. NH in each group of water was detected separately 4 + - the initial concentration of N; then add 10ppm of the above-mentioned Arthrobacter tobacco YY-9 bacterial powder to the water body of the experimental group, and the control group doe...

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Abstract

The present invention screens Arthrobacter tobacco YY-9 from the water sample in the salmon circulating aquaculture wastewater treatment tower, which has been preserved in the China Typical Culture Collection Center on June 24, 2014, and the number is CCTCC NO: M2014278. The ammoniacal nitrogen in the aquaculture wastewater has a strong degradation ability, and the removal rate of the ammoniacal nitrogen after 48 hours of treatment is as high as 92.70%, and the water purification effect is remarkable; the present invention also utilizes the above-mentioned Arthrobacter tobacco YY-9 to directly treat small shed shrimp culture ponds In the water body, after 48 hours of treatment, NH 4 + The removal rate of -N is as high as 90.28%. Therefore, the Arthrobacter tobacco YY-9 can effectively solve the problem of excessive ammoniacal nitrogen in aquaculture and reduce the pollution of high-density aquaculture to water bodies.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to an Arthrobacter tobacco strain with heterotrophic nitrosation function and application thereof. Background technique [0002] In recent years, my country's aquaculture industry has developed rapidly, and the scale of intensive and high-density farming has been expanding day by day. Concentrated feeding has resulted in the accumulation of a large amount of residual bait, feces, and animal and plant corpses in the aquaculture water body, resulting in a serious excess of nitrogen content in the water body and eutrophication. The aggravation of globalization has caused the destruction of the aquaculture ecological environment, frequent occurrence of fish and shrimp diseases, and ultimately caused huge economic losses. Nitrosation is the conversion of ammoniacal nitrogen into nitrite nitrogen, which is an important part of the nitrogen cycle in nature. With ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/06
Inventor 武玉强苟万里朱彩彩
Owner 青岛蔚蓝龙初生物科技有限公司
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