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Processing method of starch raw material and method for preparing citric acid

A technology of starchy raw materials and processing methods, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high grain consumption in citric acid fermentation, and the conversion rate needs to be improved, so as to improve the conversion rate and shorten the production time. Fermentation cycle, effect of increasing protein content

Inactive Publication Date: 2014-11-26
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the above process, the grain consumption of citric acid fermentation is relatively high, and the protein content in the enzymatic hydrolysis solution and the conversion rate of citric acid fermentation using the enzymatic hydrolysis solution need to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1) Crushing 62 tons of corn to obtain a crushed product with an average particle diameter of 400 microns. Combine the crushed product with 6.2 tons of mycelium (with a water content of 60% by weight) and 254.2m 3 The water was mixed at a temperature of 60°C and a pH of 5.2 to obtain a starch slurry, wherein the solid content of the starch slurry was 20% by weight.

[0055] 2) Mix the starch slurry obtained in step 1) with 12.1 kg of amylase (equivalent to the total weight of crushed corn and mycelium dry weight of 1 g using 7.5 enzyme activity units of amylase) to obtain a mixture, and the mixture is mixed with 150℃ steam is sprayed in the ejector (weight ratio of steam to mixture is 0.15:1), and the contact time is 5 seconds, so that the temperature of the mixture after contact with steam is 105℃, and keep it at this temperature 90 minutes;

[0056] Flash vaporize the mixture after the above steps and steam contact (vacuum degree is -0.1MPa (gauge pressure), time is 10 sec...

Embodiment 2

[0063] 1) Crushing 62 tons of corn to obtain a crushed product with an average particle diameter of 400 microns. The crushed product and 18.6 tons of mycelium (water content of 70% by weight) and 69.6m 3 The water is mixed at a temperature of 70° C. and a pH of 5.8 to obtain a starch slurry, wherein the solid content of the starch slurry is 45% by weight.

[0064] 2) Mix the starch slurry obtained in step 1) with 18.9 kg of amylase (equivalent to the total weight of crushed corn and mycelium dry weight of 1 g using 11.2 enzyme activity units of amylase) to obtain a mixture, and the mixture is mixed with Steam at 170°C is spray-contacted in an ejector (weight ratio of steam to mixture is 0.15:1), and the contact time is 3 seconds, so that the temperature of the mixture after contact with steam is 105°C, and keep it at this temperature 180 minutes;

[0065] Flash vaporize the mixture after the above steps and steam contact (vacuum degree is -0.1MPa (gauge pressure), time is 6 second...

Embodiment 3

[0072] 1) Crushing 62 tons of corn to obtain a crushed product with an average particle diameter of 400 microns. Combine the crushed product with 12.4 tons of mycelium (with a water content of 65% by weight) and 129.7m 3 The water is mixed at a temperature of 65° C. and a pH of 5.5 to obtain a starch slurry, wherein the solid content of the starch slurry is 32.5% by weight.

[0073] 2) Mix the starch slurry obtained in step 1) with 15.6 kg of amylase (equivalent to the total weight of crushed corn and mycelium dry weight 1 g using 9.4 enzyme activity units of amylase) to obtain a mixture, and the mixture is mixed with The 160℃ steam is sprayed and contacted in the ejector (the weight ratio of steam to the mixture is 0.15:1), and the contact time is 6 seconds, so that the temperature of the mixture after contact with the steam is 105℃, and keep it at this temperature 135 minutes;

[0074] Flash vaporize the mixture after the above steps are in contact with steam (vacuum degree is -...

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Abstract

The invention discloses a processing method of a starch raw material, wherein the method includes the starch raw material, mycelia and water are mixed and subjected to slurry conditioning to obtain a starch slurry, and the starch slurry, an amylase and a protease are mixed and subjected to enzymolysis, wherein the mycelia are obtained after solid liquid separation of a citric acid fermentation liquid; relative to 100 parts by weight of the starch raw material, the mycelia amount is 5-40 parts by weight, and the water amount is 50-500 parts by weight. The invention also provides a method for preparing citric acid through using the enzymatic hydrolysate obtained by the processing method. The processing method of the starch raw material can reduce grain consumption, and the protein content of the obtained enzymatic hydrolysate is high. Moreover, the obtained enzymatic hydrolysate is adopted to be subjected to citric acid fermentation, and the conversion rate of citric acid fermentation can be increased.

Description

Technical field [0001] The invention relates to a method for processing starch raw materials and a method for preparing citric acid. Background technique [0002] Citric acid is an organic acid widely used in beverage, food and medicine industries. At present, citric acid is mainly prepared by fermentation. For example, it is generally necessary to pulverize the starch material first, mix the pulverized product with water to obtain a starch slurry, and mix the starch slurry with enzymes for enzymatic hydrolysis to obtain an enzymatic hydrolysis product (enzymatic hydrolysis). Liquefaction solution), and inoculate Aspergillus niger into the fermentation broth containing the enzymatic hydrolysis product to ferment to produce citric acid. [0003] In the above process, the grain consumption of citric acid fermentation is relatively high, and the protein content in the enzymolysis solution and the conversion rate of using the enzymolysis solution for citric acid fermentation need to b...

Claims

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Application Information

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IPC IPC(8): C12P7/48C12R1/685
Inventor 李黎明罗虎黄海宁杨儒文
Owner COFCO BIOTECHNOLOGY CO LTD
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