Application of Brachypine in the Preparation of Preparations for Preventing Cerebral Ischemic Injury
A new application of breciferin and cerebral ischemia technology in food or health care products. In the field of preparation of drugs to prevent cerebral ischemic injury, brevicine can solve the problems of less research on pharmacological efficacy and activity, and achieve a large society The effect of benefits and economic benefits
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Embodiment 1
[0029] Example 1: Effects of brevicine on the viability of N2a cells damaged by OGD
[0030] N2a cells were cultured in DMEM medium containing 10% fetal bovine serum, and passaged once to three times to ensure the continuous growth of cells. N2a cells in logarithmic growth phase were taken, and the cell density was adjusted to 1×10 after digestion. 5 cells / mL, added to 96-well culture plate, 100 μL cell suspension per well, at 37°C, 5% CO 2 Incubate in the incubator for 24 hours to make the cell growth reach the best state. The N2a cells were randomly divided into normal group, model group, brachypine group and ligustrazine (drug composition for occlusive vascular disease, cerebral thrombosis, vasculitis, coronary heart disease, angina pectoris, etc.) group.
[0031] Each group was treated as follows: Ligustrazine group and Brachypine group were incubated with 7.5 μM, 15 μM, and 30 μM working solution for 24 hours, and then OGD was carried out together with the model group (w...
Embodiment 2
[0036] Example 2 Antioxidative effect of brecypine on OGD damage N2a
[0037] Take N2a cells in the logarithmic phase, with 5×10 4 cells / mL were inoculated in 24-well plates and used for experiments after pre-incubation for 12 hours.
[0038] Each group was treated as follows: Ligustrazine group and Brachypine group were incubated with 7.5 μM, 15 μM, and 30 μM working solution for 24 hours, and then OGD was carried out together with the model group (without adding samples, incubated with DMEM high-glucose medium for 24 hours) to induce N2a Cell ischemia injury: replace the original medium with sugar-free Earle solution, and pass through 95% N 2 , 5%CO 2 mixed gas, cultured in hypoxia for 4 hours, then washed three times with PBS, replaced sugar-free Earles solution with DMEM high-glucose medium, reoxygenated (5% CO 2 , 95%O 2 ), and at 5% CO 2 Incubate in the incubator for 24h. The normal group was cultured routinely, without hypoxia and glucose deficiency and reoxygenat...
Embodiment 3
[0045] Example 3 Effects of Brachypine on the Concentration of Calcium Ion [Ca2+]i in Damaged Nerve Cells
[0046] Take N2a cells in the logarithmic phase, with 5×10 4 cells / mL were inoculated in cell culture dishes and used for experiments after pre-incubation for 12 hours.
[0047] Each group was treated as follows: Ligustrazine group and Brachypine group were incubated with 7.5 μM, 15 μM, and 30 μM working solution for 24 hours, and then OGD was carried out together with the model group (without adding samples, incubated with DMEM high-glucose medium for 24 hours) to induce N2a Cell ischemia injury: replace the original medium with sugar-free Earle solution, and pass through 95% N 2 , 5%CO 2 mixed gas, cultured in hypoxia for 4 hours, then washed three times with PBS, replaced sugar-free Earles solution with DMEM high-glucose medium, reoxygenated (5% CO 2 , 95%O 2 ), and at 5% CO 2 Incubate in the incubator for 24h. The normal group was cultured routinely, without hyp...
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