Orderly porous polymer thin film, preparation method and application in capture and controllable glucose-responsive release of insulin

A porous film and polymer technology, applied in the capture and release of insulin in response to controllable sugar, in the field of polymer ordered porous film materials, can solve the problems of less attention to the film system, and achieve simple and convenient equipment and high release efficiency , the effect of mild conditions

Inactive Publication Date: 2014-12-03
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, glucose-sensitive smart hydrogels are the most widely used, but less attention has been paid to thin-film systems.

Method used

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  • Orderly porous polymer thin film, preparation method and application in capture and controllable glucose-responsive release of insulin
  • Orderly porous polymer thin film, preparation method and application in capture and controllable glucose-responsive release of insulin
  • Orderly porous polymer thin film, preparation method and application in capture and controllable glucose-responsive release of insulin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Dissolve 30mg PS (purchased from Sigma-Aldrich, molecular weight: 350,000) and 2.5mg DDA in 5mL dichloromethane solution to prepare polymer organic solution, take 50μL deionized water into 1mL volumetric flask, and then add polymer Take the organic solution to the marked line, shake it for about 1 min to obtain a microemulsion, and pour the microemulsion on a clean glass substrate under the conditions of humidity of 35% and temperature of 27°C. After the organic solvent and water were completely volatilized, an ordered porous film with a pore diameter of about 2 μm, a pore depth of 1 μm, and a film thickness of 35 μm was obtained.

[0032] The obtained ordered porous film was immersed in a 3wt% PAA solution for 30 minutes, the film was taken out, washed three times in deionized aqueous solution, and then placed in the air to dry naturally.

[0033] Soak the PAA-loaded porous film in a mixed solution of 7.5mM APBA and 12.5mM EDC for 3h, remove the film, wash it three tim...

Embodiment 2

[0040] As shown in Example 1, with other conditions unchanged, the mass of DDA was changed to 1.5 mg, and an ordered porous film with a pore diameter of about 2 μm, a pore depth of 1 μm, and a film thickness of 20 μm was prepared.

[0041] Then according to the steps of Example 1, the modification of PAA, APBA and Alg in the hole, the positioning assembly of insulin aggregates, and the in situ release study in the hole were respectively realized. Under this condition, the insulin release efficiency was 95%.

Embodiment 3

[0043] As shown in Example 1, with other conditions unchanged, the surfactant DDA was replaced by DDAB, and an ordered porous film with a pore diameter of about 4 μm, a pore depth of 2 μm, and a film thickness of 50 μm was prepared.

[0044] Then according to the steps of Example 1, the modification of PAA, APBA and Alg in the hole, the positioning assembly of insulin aggregates, and the in situ release study in the hole were respectively realized. In this case, the insulin release efficiency was 95%.

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Abstract

The invention relates to an orderly porous polymer thin film, a preparation method and application in capture and controllable glucose-responsive release of insulin and belongs to the technical field of controllable release of drugs. The preparation method comprises the following steps of preparing an orderly cellular porous thin film of which the inner wall is loaded with a positively charged surfactant, selectively assembling a glucose-responsive material on the inner walls of pores of the orderly porous thin film, carrying out positioning capture on an insulin aggregate in the pores, carrying out controllable release on the insulin aggregate under the stimulated action of glucose and the like. According to the invention, the device is simple and convenient, the condition is mild, the insulin loading capacity is large, the release efficiency is high, the positioning capture of the insulin aggregate in the pores of the orderly cellular porous thin film can be achieved, and the insulin is efficiently released by virtue of the stimulation of glucose; furthermore, by virtue of changing the concentration of glucose, the regulation on the controllable release behavior of insulin is achieved and a glucose-responsive self-regulating insulin delivery system is established.

Description

technical field [0001] The invention belongs to the technical field of drug controllable release, and in particular relates to a polymer ordered porous film material, a preparation method and its application in trapping and controllable sugar-responsive release of insulin. Background technique [0002] Diabetes mellitus is a series of clinical syndromes caused by relative or absolute insufficiency of insulin in the body, which is manifested by high blood glucose concentration (Mark A.A., George S.E., Lancet 2001, 358, 221–229). At present, the treatment of diabetes mainly relies on insulin injections to maintain a normal life, which causes unavoidable pain for patients. Therefore, it is very important to develop a self-regulating drug delivery system that has characteristics similar to artificial pancreas, that is, can release insulin according to blood sugar levels, for the treatment of such diseases. At present, there are mainly three types of typical glucose-responsive m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08J9/28C08J7/00C08L25/06C08L33/12C08J3/02A61K47/32A61K47/36A61K38/28A61K9/70A61P3/10
Inventor 梁静马英一吴立新
Owner JILIN UNIV
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