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Metolcarb piezoelectric immunosensor and preparation method thereof

An immune sensor and piezoelectric technology, which is applied in the field of sensing and immune analysis, can solve the problems of sensor signal instability and poor repeatability, and achieve the effects of high selective recognition ability, low cost and high detection sensitivity

Inactive Publication Date: 2014-12-10
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Multi-walled carbon nanotubes have large specific surface area and good biocompatibility, and are often used as modified substrates for bioactive molecules in biosensors. poor repeatability

Method used

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  • Metolcarb piezoelectric immunosensor and preparation method thereof
  • Metolcarb piezoelectric immunosensor and preparation method thereof
  • Metolcarb piezoelectric immunosensor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Pretreatment of quartz crystal gold electrodes

[0024] After the quartz crystal gold electrode was cleaned with absolute ethanol, it was immersed in 30% H 2 o 2 with 98% H 2 SO 4 Put in the mixed solution (1:3, v / v) for 5 minutes, take it out, wash it with plenty of water and ethanol, and dry it for later use.

[0025] 2. Preparation of Memetcarb Piezoelectric Immunosensor

[0026] (1) 0.5 g of multi-walled carbon nanotubes was heated to reflux in 20 ml of concentrated nitric acid / concentrated sulfuric acid mixture (1 / 3, v / v) for 2 hours, rinsed with secondary water until neutral, and dried for later use.

[0027] (2) Take 5.0 mg of carboxylated multi-walled carbon nanotubes and ultrasonically disperse them in water, add 100 mg each of EDC and NHS, add 1.0 ml of methanol solution containing 25 mg of polyamide-amine dendrimers (4.0 generation), and stir at room temperature 2 hours. The mixture was transferred to a dialysis bag (molecular weight range: 8000-1400...

Embodiment 2

[0031] 1. Pretreatment of quartz crystal gold electrodes

[0032] After the quartz crystal gold electrode was cleaned with absolute ethanol, it was immersed in 30% H 2 o 2 with 98% H 2 SO 4 Put in the mixed solution (1:3, v / v) for 5 minutes, take it out, wash it with plenty of water and ethanol, and dry it for later use.

[0033] 2. Preparation of Memetcarb Piezoelectric Immunosensor

[0034] (1) 0.5 g of multi-walled carbon nanotubes was heated to reflux in 20 ml of concentrated nitric acid / concentrated sulfuric acid mixture (1 / 3, v / v) for 3 hours, rinsed with secondary water until neutral, and dried for later use.

[0035] (2) Take 5.0 mg of carboxylated multi-walled carbon nanotubes and ultrasonically disperse them in water, add 100 mg each of EDC and NHS, add 1.0 ml of methanol solution containing 25 mg of polyamide-amine dendrimers (4.0 generation), and stir at room temperature 2 hours. The mixture was transferred to a dialysis bag (molecular weight range: 8000-1400...

Embodiment 3

[0039] 1. Pretreatment of quartz crystal gold electrodes

[0040] After the quartz crystal gold electrode was cleaned with absolute ethanol, it was immersed in 30% H 2 o 2 with 98% H 2 SO 4 Put in the mixed solution (1:3, v / v) for 5 minutes, take it out, wash it with plenty of water and ethanol, and dry it for later use.

[0041] 2. Preparation of Memetcarb Piezoelectric Immunosensor

[0042](1) 0.5 g of multi-walled carbon nanotubes was heated to reflux in 20 ml of concentrated nitric acid / concentrated sulfuric acid mixture (1 / 3, v / v) for 4 hours, rinsed with secondary water until neutral, and dried for later use.

[0043] (2) Take 5.0 mg of carboxylated multi-walled carbon nanotubes and ultrasonically disperse them in water, add 100 mg each of EDC and NHS, add 1.0 ml of methanol solution containing 25 mg of polyamide-amine dendrimers (4.0 generation), and stir at room temperature 2 hours. The mixture was transferred to a dialysis bag (molecular weight range: 8000-14000...

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Abstract

The invention provides a metolcarb piezoelectric immunosensor and a preparation method thereof. According to the metolcarb piezoelectric immunosensor, a multi-walled carbon nanotube and polyamide-amine dendrimer compound is dispensed on the surfaces of gold electrodes of piezoelectric quartz crystals, and a metolcarb polyclonal antibody is self-assembled and fixed by virtue of a large quantity of active amino groups on the surfaces of dendrimers, so that metolcarb in carbamate pesticides is directly and quantitatively detected. By virtue of the metolcarb piezoelectric immunosensor, the characteristics of high specific surface area of multi-walled carbon nanotubes and high-sensitivity detection of the active groups on the surfaces of the polyamide-amine dendrimers and a quartz crystal microbalance are effectively combined; the metolcarb piezoelectric immunosensor is simple to prepare; the conditions are liable to control; the prepared piezoelectric sensor has the advantages of high sensitivity and high reproducibility, and can be used for analyzing and detecting metolcarb in samples such as apple juice and orange juice.

Description

technical field [0001] The invention relates to the technical field of sensing and immunoanalysis, in particular to a metcarb piezoelectric immunosensor and a preparation method thereof. Background technique [0002] Metolcarb (Metolcarb) is a quick-acting carbamate insecticide. The pure product is a white crystalline powder. It is insoluble in water and easily soluble in organic solvents such as methanol and acetone. It is very stable under alkaline and high temperature conditions. easy to break down. At present, it is widely used in the pest control of rice fields, vegetables, fruits and other crops. It has contact killing, fumigating and systemic effects, and is a high-efficiency and low-toxicity insecticide. The semi-lethal dose to mammals (in terms of mice) is 268mg·kg -1 . After eating food contaminated with Methiocarb, it will easily accumulate in the organism, posing a potential threat. So far, the detection methods of Methiocarb commonly used at home and abroad ...

Claims

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Application Information

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IPC IPC(8): G01N33/544
CPCG01N33/544
Inventor 王硕王俊平潘明飞
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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